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鳖甲煎改良方下调
CTGF /Itgα 5对肝星状细胞T6周期变化、细胞凋亡及Col 1a 1表达的影响Effect of novel turtle shell decoction on cycle, apoptosis,
CTGF /Itgα 5 pathway and expression ofCol 1a 1 in rat hepatic stellate cells- 第三军医大学学报 2021年43卷第24期 页码：2664-2670
- 作者机构：
1.400038 重庆，陆军军医大学(第三军医大学)第一附属医院中医与风湿免疫科
2.841000 新疆维吾尔自治区 库尔勒，解放军951医院心肾呼吸科
- 作者简介：
[ 张荣华，E-mail：zhrhlggg@163.com ZHANG Ronghua, E-mail: zhrhlggg@163.com
- 基金信息：基础研究军民融合项目(SWH2017JCZD-5)
DOI：10.16016/j.1000-5404.202106056
中图分类号： R285.5;R289.5;R575.5收稿：2021-06-07，
修回：2021-07-04，
纸质出版：2021-12-30
- 稿件说明：
移动端阅览
蔡秀华, 董岸莺, 张荣华, 等. 鳖甲煎改良方下调
CTGF /Itgα 5对肝星状细胞T6周期变化、细胞凋亡及Col 1a 1表达的影响[J]. 第三军医大学学报, 2021,43(24):2664-2670.Xiuhua CAI, Anying DONG, Ronghua ZHANG, et al. Effect of novel turtle shell decoction on cycle, apoptosis,
CTGF /Itgα 5 pathway and expression ofCol 1a 1 in rat hepatic stellate cells[J]. Journal of Third Military Medical University, 2021, 43(24): 2664-2670.蔡秀华, 董岸莺, 张荣华, 等. 鳖甲煎改良方下调
CTGF /Itgα 5对肝星状细胞T6周期变化、细胞凋亡及Col 1a 1表达的影响[J]. 第三军医大学学报, 2021,43(24):2664-2670. DOI： 10.16016/j.1000-5404.202106056.Xiuhua CAI, Anying DONG, Ronghua ZHANG, et al. Effect of novel turtle shell decoction on cycle, apoptosis,
CTGF /Itgα 5 pathway and expression ofCol 1a 1 in rat hepatic stellate cells[J]. Journal of Third Military Medical University, 2021, 43(24): 2664-2670. DOI： 10.16016/j.1000-5404.202106056.
摘要
目的
2
探讨鳖甲煎改良方(novel turtle shell decoction，NTSD)对肝星状细胞T6(hepatic stellate cell T6，HSCT6)的周期、凋亡、结缔组织生长因子(
connective tissue growth factor
，
CTGF
)/整合素
α
5(
integrin alpha
5，
Itgα
5)通路及1型胶原(
Collagen type
1，
Col
1
a
1)表达的影响。
方法
2
以NTSD(含生药量为0.71 g/mL)处理的HSCT6为实验(NTSD)组，鳖甲煎丸(turtle shell pills，TSP，与NTSD相同剂量)处理的HSCT6作为阳性对照(TSP)组，未处理的HSCT6为正常对照组。采用流式仪(FCM)检测各组HSCT6细胞的周期、凋亡变化。定量PCR、Western blot及免疫荧光细胞化学技术分别检测各组HSCT6细胞的
CTGF
、
Itgα
5及
Col
1
a
1的mRNA及其蛋白的表达。
结果
2
FCM检测结果显示，NTSD可将HSCT6阻滞于S细胞周期，并诱导其凋亡。定量PCR、Western blotting及免疫荧光细胞化学技术检测结果显示，NTSD可在mRNA与蛋白水平显著下调HSCT6的
CTGF
、
Itgα
5及
Col
1
a
1的表达，差异均有统计学意义(
P
<
0.01)。
结论
2
NTSD可通过下调
CTGF
/
Itgα
5将HSCT6阻滞于S细胞周期，诱导其凋亡，并影响其
Col
1
a
1的表达，以抑制其生长。
Abstract
Objective
2
To investigate the effects of novel turtle shell decoction (NTSD) on the cycle
apoptosis
connective tissue growth factor
/
integrin alpha
5 (
CTGF
/
Itgα
5) pathway and the expression of
Collagen type I
(
Col
1
a
1) in rat hepatic stellate cell line T6 (HSCT6).
Methods
2
HSCT6 cells was treated with NTSD containing crude drug 0.71 g/mL as experimental group
treated with turtle shell pills (TSP) at the same
dose as positive control
and cultured routinely as normal control. Cell cycle and apoptosis of HSCT6 cells were tested by flow cytometry (FCM). Real time-qPCR
Western blotting and immunofluorescence assay were employed to detect the expression changes of
CTGF
Itgα
5 and
Col
1
a
1 genes and their coding proteins in each group of HSCT6
respectively.
Results
2
FCM showed that NTSD treatment blocked the cells at S stage
and induced cell apoptosis. Real time-qPCR
Western blotting and immunofluorescence assay revealed that NTSD can significantly down-regulate the expression of
CTGF
Itgα
5 and
Col
1
a
1 in mRNA and protein levels in HSC-T6 cells (
P
<
0.01).
Conclusion
2
NTSD treatment results in cell arrest at S phage through down-regulating
CTGF
/
Itgα
5
induction of cell apoptosis
decrease of
Col
1
a
1 expression
and then inhibition of cell growth in HSCT6 cells.
关键词
Keywords
references
CHEN W, LI Y, HSU C T, et al . Connective tissue growth fac tor in hepatocytes is elevated by carbon tetrachloride via STAT3 activation[J ] . Mol Med Rep, 2020, 21(3): 1390-1398. DOI: 10.3892/mmr.2020.10916.
SEO H Y, LEE S H, LEE J H, et al . Src inhibition attenuates liver fibrosis by preventing hepatic stellate cell activation and decreasing connective tissue growth factor[J ] . Cell, 2020, 9(3): 558. DOI: 10.3390/cells9030558.
SEO H Y, JUNG Y A, LEE S H, et al . Kahweol decreases hepatic fibrosis by inhibiting the expression of connective tissue growth factor via the transforming growth factor-beta signal- ing pathway[J ] . Oncotarget, 2017, 8(50): 87086-87094. DOI: 10.18632/oncotarget.19756.
VERBEKE L, MANNAERTS I, SCHIERWAGEN R, et al . FXR agonist obeticholic acid reduces hepatic inflammation and fibrosis in a rat model of toxic cirrhosis[J ] . Sci Rep, 2016, 6: 33453. DOI: 10.1038/srep33453.
PI L, ROBINSON P M, JORGENSEN M, et al . Connective tissue growth factor and integrin αvβ6: a new pair of regulators critical for ductular reaction and biliary fibrosis in mice[J ] . Hepatology, 2015, 61(2): 678-691. DOI: 10.1002/hep.27425.
KIM J, KANG W, KANG S H, et al . Proline-rich tyrosine kinase 2 mediates transforming growth factor-beta-inducedhepatic stellate cell activation and liver fibrosis[J ] . Sci Rep, 2020, 10(1): 21018. DOI: 10.1038/s41598-020-78056-0.
MU M, ZUO S, WU R M, et al . Ferulic acid attenuates liver fibrosis and hepatic stellate cell activation via inhibition of TGF-β/Smad signaling pathway[J ] . Drug Des Devel Ther, 2018, 12: 4107-4115. DOI: 10.2147/dddt.s186726.
CHENG Q, LI C, YANG C F, et al . Methyl ferulic acid attenuates liver fibrosis and hepatic stellate cell activation through the TGF-β1/Smad and NOX4/ROS pathways[J ] . Chem Biol Interact, 2019, 299: 131-139. DOI: 10.1016/j.cbi.2018.12.006.
彭涛. 鳖甲煎丸在肝癌治疗中的临床应用[J]. 中西医结合肝病杂志, 2020, 30(6): 481-483.
PENG T. Clinical application of turtle shell pills in the treatment of liver cancer[J]. Chin J Integr Tradit West Med Liver Dis, 2020, 30(6): 481-483.
BAI G P, YAN G H, WANG G J, et al . Anti-hepatic fibrosis effects of a novel turtle shell decoction by inhibiting hepatic stellate cell proliferation and blocking TGF-β1/Smad signaling pathway in rats[J ] . Oncol Rep, 2016, 36(5): 2902-2910. DOI: 10.3892/or.2016.5078.
艾志波, 张荣华, 闫国和, 等. 鳖甲煎改良方对大鼠肝纤维化的作用及其机制研究[J]. 第三军医大学学报, 2011, 33(3): 274-277. DOI: 10.16016/j.1000-5404.2011.03.004.
AI Z B, ZHANG R H, YAN G H, et al . Effect of modified turtle shell decoction on hepatic fibrosis in rats and its mechanism[J ] . J Third Mil Med Univ, 2011, 33(3): 274-277. DOI: 10.16016/j.1000-5404.2011.03.004.
艾志波, 张荣华, 闫国和. 鳖甲煎改良方对大鼠肝纤维化的防治作用[J]. 世界华人消化杂志, 2011, 19(1): 13-18.
AI Z B, ZHANG R H, YAN G H, et al . A modified recipe of turtle shell decoction reduces experimental hepatic fibrosis in rats[J ] . World Chin J Dig, 2011, 19(1): 13-18.
柏干苹, 张荣华, 闫国和, 等. 鳖甲煎改良方对肝星状细胞生长及TGFβ1、Smad3与Smad7表达的影响[J]. 免疫学杂志, 2017, 33(9): 777-782. DOI: 10.13431/j.cnki.immunol.j.20170137.
BAI G P, ZHANG R H, YAN G H, et al . The effect of modified turtle shell decoction on the expression of TGFβ1, Smad3 and Smad7 in rat hepatic stellate cell[J ] . Immunol J, 2017, 33(9): 777-782. DOI: 10.13431/j.cnki.immunol.j.20170137.
TSUCHIDA T, LEE Y A, FUJIWARA N, et al . A simple diet- and chemical-induced murine NASH model with rapid progression of steatohepatitis, fibrosis and liver can cer[J ] . J Hepatol, 2018, 69(2): 385-395. DOI: 10.1016/j.jhep.2018.03.011.
AFFO S, YU L X, SCHWABE R F. The role of cancer-associated fibroblasts and fibrosis in liver cancer[J]. Annu Rev Pathol, 2017, 12: 153-186. DOI: 10.1146/annurev-pathol-052016-100322.
MALDONADO H, HAGOOD J S. Cooperative signaling between integrins and growth factor receptors in fibrosis[J]. J Mol Med (Berl), 2021, 99(2): 213-224. DOI: 10.1007/s00109-020-02026-2.
SMITH-CORTINEZ N, FAGUNDES R R, GOMEZ V, et al . Collagen release by human hepatic stellate cells requires vitamin C and is efficiently blocked by hydroxylase inhibition[J ] . FASEB J, 2021, 35(2): e21219. DOI: 10.1096/fj.202001564RR.
YU B, JIN G N, MA M, et al . Taurocholate induces connective tissue growth factor expression in hepatocytes through ERK-YAP signaling[J ] . Cell Physiol Biochem, 2018, 50(5): 1711-1725. DOI: 10.1159/000494790.
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