长链非编码RNA DUXAP8通过调控miR-4677-3p对卵巢癌细胞增殖、周期和凋亡的影响

陈岑; 苏丹; 李宁蔚; 江琴; 王亚洲

doi:10.16016/j.1000-5404.202106001

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长链非编码RNA DUXAP8通过调控miR-4677-3p对卵巢癌细胞增殖、周期和凋亡的影响

基础医学 | 更新时间：2026-01-09

- 长链非编码RNA DUXAP8通过调控miR-4677-3p对卵巢癌细胞增殖、周期和凋亡的影响
- Effect of long non-coding RNA DUXAP8 on proliferation, cycle and apoptosis of ovarian cancer cells by regulating miR-4677-3p
- 第三军医大学学报 2021年43卷第24期页码：2656-2663
- 作者机构：
  
  1.610072 成都，四川省人民医院妇产科
  2.611130 成都，成都市第五人民医院外科
- 作者简介：
  
  [ 王亚洲，E-mail：1115325527@qq.com WANG Yazhou, E-mail: 1115325527@qq.com
- 基金信息：
  
  成都市科技局-技术创新研发项目(2019-YF05-00244-SN)
- DOI：10.16016/j.1000-5404.202106001
  中图分类号： R394.3;R730.23;R737.31
- 收稿：2021-06-01，
  
  修回：2021-09-26，
  
  纸质出版：2021-12-30
- 稿件说明：
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陈岑, 苏丹, 李宁蔚, 等. 长链非编码RNA DUXAP8通过调控miR-4677-3p对卵巢癌细胞增殖、周期和凋亡的影响[J]. 第三军医大学学报, 2021,43(24):2656-2663.

Cen CHEN, Dan SU, Ningwei LI, et al. Effect of long non-coding RNA DUXAP8 on proliferation, cycle and apoptosis of ovarian cancer cells by regulating miR-4677-3p[J]. Journal of Third Military Medical University, 2021, 43(24): 2656-2663.
陈岑, 苏丹, 李宁蔚, 等. 长链非编码RNA DUXAP8通过调控miR-4677-3p对卵巢癌细胞增殖、周期和凋亡的影响[J]. 第三军医大学学报, 2021,43(24):2656-2663. DOI： 10.16016/j.1000-5404.202106001.

Cen CHEN, Dan SU, Ningwei LI, et al. Effect of long non-coding RNA DUXAP8 on proliferation, cycle and apoptosis of ovarian cancer cells by regulating miR-4677-3p[J]. Journal of Third Military Medical University, 2021, 43(24): 2656-2663. DOI： 10.16016/j.1000-5404.202106001.

摘要

目的

探讨长链非编码RNA DUXAP8通过调控miR-4677-3p对卵巢癌细胞的增殖、周期和凋亡的影响。

方法

通过RT-qPCR检测卵巢癌组织和细胞中的DUXAP8和miR-4677-3p的表达。然后将SKOV3细胞分为：si-NC组、si-DUXAP8组、miR-NC组、miR-4677-3p组、si-DUXAP8+anti-miR-NC组和si-DUXAP8+anti-miR-4677-3p组；CCK8、克隆形成实验检测各组细胞活力；流式细胞术检测各组细胞周期和凋亡情况；Western blot检测Cyclin D1、Bcl-2和Bax蛋白表达；双荧光素酶报告实验检测DUXAP8和miR-4677-3p的关系。

结果

与癌旁组织和正常卵巢上皮细胞比较，DUXAP8在卵巢癌组织(0.98±0.25)

(4.14±0.72)中表达明显增加，SKOV3、OV90、CaOV3表达明显增加(1.03±0.08

3.26±0.33、2.75±0.29、2.54±0.26)，差异具有统计学意义(

0.05)。干扰DUXAP8或过表达miR-4677-3p可以抑制卵巢癌细胞的增殖、降低凋亡率、阻滞细胞周期，差异具有统计学意义(

0.05)。DUXAP8调控miR-4677-3p的表达，抑制miR-4677-3p可以部分回复干扰DUXAP8对卵巢癌细胞增殖、周期和凋亡的作用，差异具有统计学意义(

0.05)。

结论

干扰DUXAP8能够抑制卵巢癌细胞增殖、诱导细胞凋亡、阻滞细胞周期，其作用机制可能与上调miR-4677-3p有关。

Abstract

Objective

To investigate the effects of long non-coding RNA DUXAP8 on the proliferation

cell cycle and apoptosis of ovarian cancer cells by regulating miR-4677-3p.

Methods

The expression of DUXAP8 and miR-4677-3p in ovarian cancer tissues and cell lines (SKOV3

OV90 and CaOV3) was detected by RT-qPCR. Then SKOV3 cells were divided into 6 groups

namely

si-NC group

si-DUXAP8 group

miR-NC group

miR-4677-3p group

si-DUXAP8+anti-miR-NC group and si-DUXAP8+anti-miR-4677-3p group. CCK-8 assay and clone formation assay were adopted to detect cell viability

flow cytometry to observe cell apoptosis and cycle

Western blotting to measure the expression levels of Cyclin D1

Bcl-2 and Bax

and dual luciferase reporter assay system to analyze the relationship of DUXAP8 and miR-4677-3p.

Results

When compared with adjacent tissues and normal ovarian epithelial cells

the expression level of DUXAP8 was significantly lower in ovarian cancer tissues (0.98±0.25

4.14±0.72) and that of SKOV3

OV90

CaOV3 was obviously higher (1.03±0.08

3.26±0.33、2.75±0.29、2.54±0.26

all

0.05). Interference with DUXAP8 or overexpression of miR-4677-3p inhibited the proliferation

reduced the rate of apoptosis

and arrested the cell cycle of ovarian cancer cells (

0.05). DUXAP8 regulated the expression of miR-4677-3p

and inhibiting miR-4677-3p partially restore and interfere with the effects of DUXAP8 on the proliferation

cycle and apoptosis of ovarian cancer cells (

0.05).

Conclusion

Interference with DUXAP8 can inhibit the proliferation of ovarian cancer cells

induce cell apoptosis

and block cell cycle

the mechanism may be related to the up-regulation of miR-4677-3p.

关键词

Keywords

references

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