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1例复发类鼻疽的病原学鉴定及生物学特性研究
Etiological identification and biological characteristics of recurrent melioidosis: report of 1 case
- 第三军医大学学报 2021年43卷第22期 页码：2441-2448
- 作者机构：
1.400038 重庆，陆军军医大学（第三军医大学）药学与检验医学系临床微生物与免疫学教研室
2.430070 湖北，华中农业大学生命科学技术学院
3.572000 海南，三亚市人民医院检验科
- 作者简介：
[ 毛旭虎，E-mail：mxh2012@hotmail.com MAO Xuhu, E-mail:mxh2012@hotmail.com
- 基金信息：国家自然科学基金面上项目(81971907)
DOI：10.16016/j.1000-5404.202105139
中图分类号： R372;R378.99;R515.9收稿：2021-05-26，
修回：2021-08-31，
纸质出版：2021-11-30
- 稿件说明：
移动端阅览
杨文波, 张纳安, 饶承龙, 等. 1例复发类鼻疽的病原学鉴定及生物学特性研究[J]. 第三军医大学学报, 2021,43(22):2441-2448.
Wenbo YANG, Na'an ZHANG, Chenglong RAO, et al. Etiological identification and biological characteristics of recurrent melioidosis: report of 1 case[J]. Journal of Third Military Medical University, 2021, 43(22): 2441-2448.
杨文波, 张纳安, 饶承龙, 等. 1例复发类鼻疽的病原学鉴定及生物学特性研究[J]. 第三军医大学学报, 2021,43(22):2441-2448. DOI： 10.16016/j.1000-5404.202105139.
Wenbo YANG, Na'an ZHANG, Chenglong RAO, et al. Etiological identification and biological characteristics of recurrent melioidosis: report of 1 case[J]. Journal of Third Military Medical University, 2021, 43(22): 2441-2448. DOI： 10.16016/j.1000-5404.202105139.
摘要
目的
2
通过对1例复发类鼻疽进行系统病原学鉴定及生物学特性研究，分析和探讨类鼻疽菌长期滞留机体可能的适应宿主机制。
方法
2
对临床收集的来源于同一个患者时隔5年分离到的两株类鼻疽菌，进行微生物学鉴定、全基因组测序，并使用相关软件对测序数据进行多位点序列分型（multilocus sequence typing，MLST）、同源比对进化树、单核苷酸多态性（single nucleotide polymorphism，SNP）以及插入缺失（insertion-deletion，Indel）分析；进一步在感染肺上皮A549细胞模型中比较感染不同时间细胞内细菌载量变化；双向电泳-质谱技术分析两株细菌在蛋白水平的表达变化。
结果
2
分离到的两株类鼻疽菌，全基因组均约7.12 Mb，COG功能注释编码蛋白大致约5 000个；MLST分型均为ST58型；同源比对进化树分析显示它们位于同一分支且与其他分离自中国的类鼻疽菌亲缘关系较近；SNP/Indel分析显示复发株有114个SNP位点、55个Indel位点；与原发株相比，双向电泳-质谱结果表明复发株共有68个蛋白的表达水平发生了显著的变化；同时复发株感染A549后细胞内细菌载量明显增加（
P
<
0.05）。
结论
2
以上复发菌株的基因组学、蛋白组学以及胞内感染增殖能力的系统研究提示类鼻疽菌可能在机体长期的选择压力下微进化，通过对细菌基因转录表达、分泌系统、耐药以及能量代谢等方面的调控更加适应其在宿主体内的生存而实现长期滞留。
Abstract
Objective
2
To analyze and explore the possible adaptive mechanisms that Burkholderia
pseudomallei
(B.
pseudomallei
) could long-term reside in the host cells based on the systematic etiological identification and biological characteristics of a patient with recurrent melioidosis.
Methods
2
The 2 B.
pseudomallei
strains isolated from the same patient at an interval of 5 years were performed for microbiological identification and genome sequencing
and the sequencing data were further analyzed by multilocus sequence typing (MLST)
phylogenetic tree analysis of homologous alignment
single nucleotide polymorphism (SNP) analysis and insertion-deletion (Indel) analysis
respectively. Furthermore
the intracellular survival of the 2 clinical isolates of B.
pseudomallei
were examined at different times by counting the viable bacteria in the A549 cells. Two-dimensional electrophoresis (2-DE) and mass spectrometry was employed to analyze the protein expression of 2 strains of bacteria.
Results
2
The 2 clinical isolates had a whole genome of about 7.12 Mb
and up about 5 000 Clusters of Orthologous Groups (COG) annotations
and was identified as sequence type (ST) 58 by MLST analysis. Phylogenetic analysis of homologous alignment showed that they were located in the same branch and were closely related to other B.
pseudomallei
isolates from China. SNP/Indel analysis indicated that there were 114 SNP sites and 55 Indel sites in the recurrent strain. The results of 2-DE displayed that the expression levels of 68 proteins in the recurrent strain were significantly changed compared with the primary strain. The intracellular bact
erial load of recurrent strain was significantly increased compared to the primary strain in A549 cells(
P
<
0.05).
Conclusion
2
Based on a series of studies with genomics
proteomics and ability of intracellular survival of the 2 clinical isolates of
B
.
pseudomallei
the bacterium may evolve slightly under the long-term selective pressure of the organism
and adapt to the host environment for its own survival through the regulation of bacterial transcription
secretion system
drug resistance and energy metabolism.
关键词
Keywords
references
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