全胃肠外营养对幼鼠免疫功能和炎症反应的影响

沈蕾蕾; 孙晓东; 郑瑞雪; 赵庆宁; 青旭容; 陈盛

doi:10.16016/j.1000-5404.202105039

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全胃肠外营养对幼鼠免疫功能和炎症反应的影响

基础医学 | 更新时间：2026-01-09

- 全胃肠外营养对幼鼠免疫功能和炎症反应的影响
- Effects of total parenteral nutrition on immune function and inflammatory response in young rats
- 第三军医大学学报 2021年43卷第21期页码：2358-2365
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)第一附属医院: 儿科
  2.400038 重庆，陆军军医大学(第三军医大学)第一附属医院：病理科，全军临床病理学研究所
- 作者简介：
  
  [陈盛，E-mail：562437300@qq.com CHEN Sheng, E-mail 562437300@qq.com
- 基金信息：
  
  陆军军医大学科技创新能力提升专项(2019XQY09)
- DOI：10.16016/j.1000-5404.202105039
  中图分类号： R-332;R392.1;R459.3
- 收稿：2021-05-11，
  
  修回：2021-07-14，
  
  纸质出版：2021-11-15
- 稿件说明：
移动端阅览
沈蕾蕾, 孙晓东, 郑瑞雪, 等. 全胃肠外营养对幼鼠免疫功能和炎症反应的影响[J]. 第三军医大学学报, 2021,43(21):2358-2365.

Leilei SHEN, Xiaodong SUN, Ruixue ZHENG, et al. Effects of total parenteral nutrition on immune function and inflammatory response in young rats[J]. Journal of Third Military Medical University, 2021, 43(21): 2358-2365.
沈蕾蕾, 孙晓东, 郑瑞雪, 等. 全胃肠外营养对幼鼠免疫功能和炎症反应的影响[J]. 第三军医大学学报, 2021,43(21):2358-2365. DOI： 10.16016/j.1000-5404.202105039.

Leilei SHEN, Xiaodong SUN, Ruixue ZHENG, et al. Effects of total parenteral nutrition on immune function and inflammatory response in young rats[J]. Journal of Third Military Medical University, 2021, 43(21): 2358-2365. DOI： 10.16016/j.1000-5404.202105039.

摘要

目的

探讨全胃肠外营养(total parenteral nutrition，TPN)对幼鼠免疫功能及全身炎症的影响。

方法

将18只6~8周龄的SD雄性幼鼠分为3组：正常组、对照组和TPN组，每组6只。正常组不予置管，对照组幼鼠行右颈静脉置管后予以生理盐水治疗，对照组和正常组幼鼠均喂食标准鼠粮和水，TPN组幼鼠行右颈静脉置管后予以TPN治疗，并禁食、禁水，14 d后处死幼鼠。采用全自动流式血细胞计数仪检测外周血的血常规，流式细胞术检测脾脏细胞中HLA-DR表达、外周血Treg细胞比例、内毒素诱导单核细胞产生TNF-α的能力、淋巴细胞(CD4

、CD8

T)凋亡以及用植物血凝素(phytohemagglutinin，PH

A)刺激后产生IFN-γ的能力，ELISA法检测外周血血浆中IL-1β、TNF-α、IL-10、IL-4水平。

结果

与正常组及对照组比较，TPN组幼鼠静脉血淋巴细胞数量减少(

0.05)，脾脏细胞HLA-DR表达降低(

0.05)，TPN组幼鼠外周血Treg细胞的比例(8.54±1.20)明显高于正常组(3.02±1.49) 及对照组(3.00±0.75)幼鼠，差异有统计学意义(

0.05)。TPN组幼鼠经内毒素诱导产生TNF-α的细胞所占比例(8.73±3.11)明显低于正常组(39.07±16.17)及对照组(43.23±18.40)，而CD4

T淋巴细胞经PHA刺激后所产生IFN-γ的百分比TPN组(0.65±0.46)明显少于正常组(2.94±1.62)与对照组(2.97±1.39)，差异均有统计学意义(

0.05)，TPN组幼鼠淋巴细胞(CD4

、CD8

T)晚期凋亡增多(

0.05)，IL-1β、TNF-α水平增加(

0.05)而IL-10、IL-4水平降低(

0.05)。

结论

持续2周的TPN可导致幼鼠先天性及获得性免疫功能受损，促炎因子增加，尽早开始肠内营养对于减少免疫损伤及全身炎症具有重要意义。

Abstract

Objectives

To investigate the effects of total parenteral nutrition (TPN) on immune function and systemic inflammation in young rats.

Methods

Eighteen young male SD rats aged 6 to 8 weeks were randomly divided into 3 groups: normal group

control group and TPN group

with 6 rats in each group. The rats in the normal group were not catheterized

and those in the control group were treated with normal saline after right jugular vein catheterization. The rats in the control group and normal group were fed standard food and water

and those in the TPN group were treated with TPN after right jugular vein catheterization

fasting and water abdication. All the young rats were sacrificed in 14 d later. The blood routine of peripheral blood was detected by automatic flow cytometry. The expression of HLA-DR in spleen cells

the proportion of Treg cells in peripheral blood

the ability of endotoxin-induced monocytes to produce TNF-α

apoptosis of lymphocytes (CD4

CD8

T cells) and the ability of IFN-γ production after stimulation by phytohemagglutinin (PHA) were detected by flow cytometry. The levels of

IL-1β

TNF-α

IL-10 and IL-4 in peripheral blood plasma were determined by ELISA.

Results

Compared with normal group and control group

the number of venous blood lymphocytes was decreased (

0.05)

the HLA-DR expression level in spleen cells was reduced (

0.05)

the proportion of Treg cells in peripheral blood was significantly higher in the TPN group (8.54±1.20) than that in the normal group (3.02±1.49) and the control group (3.00±0.75) (

0.05). The proportion of TNF-α induced by endotoxin was significantly lower in the TPN group (8.73±3.11) than the normal group (39.07±16.17) and control group (43.23±18.40). The percentage of IFN-γ produced by CD4

T lymphocytes stimulated by PHA was significantly lower in the TPN group (0.65±0.46) than the normal group (2.94±1.62) and the control group (2.97±1.39)

and the difference was statistically significant (

0.05). The TPN group had increased apoptosis of lymphocytes (CD4

CD8

T cells) at late stage (

0.05)

increased IL-1β and TNF-α levels (

0.05) while decreased IL-10 and IL-4 levels (

0.05).

Conclusion

TPN sustained for 2 weeks can lead to congenital and acquired immune impairment and increase of pro-inflammatory factors in young mice. Early initiation of enteral nutrition is of great significance to reduce immune injury and systemic inflammation.

关键词

Keywords

references

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