单细胞测序在造血系统研究中的应用与展望

王军平; 陈乃成

doi:10.16016/j.1000-5404.202105013

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单细胞测序在造血系统研究中的应用与展望

专家述评 | 更新时间：2026-01-09

- 单细胞测序在造血系统研究中的应用与展望
- Single cell sequencing in the study of hematopoietic system: application and perspects
- 第三军医大学学报 2021年43卷第17期页码：1603-1610
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家杰出青年科学基金(81725019);国家自然科学基金重点项目(81930090);军队后勤科研重大专项(ALJ19J002)
- DOI：10.16016/j.1000-5404.202105013
  中图分类号：
- 收稿：2021-05-07，
  
  纸质出版：2021-09-15
- 稿件说明：
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王军平, 陈乃成. 单细胞测序在造血系统研究中的应用与展望[J]. 第三军医大学学报, 2021,43(17):1603-1610.

Junping WANG, Naicheng CHEN. Single cell sequencing in the study of hematopoietic system: application and perspects[J]. Journal of Third Military Medical University, 2021, 43(17): 1603-1610.
王军平, 陈乃成. 单细胞测序在造血系统研究中的应用与展望[J]. 第三军医大学学报, 2021,43(17):1603-1610. DOI： 10.16016/j.1000-5404.202105013.

Junping WANG, Naicheng CHEN. Single cell sequencing in the study of hematopoietic system: application and perspects[J]. Journal of Third Military Medical University, 2021, 43(17): 1603-1610. DOI： 10.16016/j.1000-5404.202105013.

摘要

目的

单细胞测序(single cell sequencing，SCS)是一项在细胞个体层面进行精准化分析的技术。它提供了全新的角度去识别单个细胞的种类、功能、状态，以及与其他细胞的相互关联，极大深化了人们对细胞异质性、基因与分子功能多样性等方面的理解。随着各个技术环节的不断优化，SCS技术已广泛应用于多个研究领域，其中造血系统研究便是该技术运用的范例。SCS不仅在基础研究中，协助完成了生理和病理生理条件下的相关造血细胞图谱的构建，探索了血细胞发育分化的可能路径，而且在临床诊治过程中，有效推动疾病发生、进展和转归等机制的破解，促进个体疗效差异显著、易于复发等现象的潜在因素的挖掘。现就SCS的技术概况、发展趋势及其在造血系统的应用进行评述，以期为后续SCS技术的积极与合理应用提供参考。

Abstract

Single cell sequencing (SCS) is a technique for precise analysis of cells at an individual level

which provides a new perspective on the identification of individual cell types

functions

states and interactions with other cells

and greatly deepens the understanding of cellular heterogeneity and genetic and molecular functional diversity. With the continuous optimization of various technical aspects

SCS technology has been widely used in many research areas

and the study of the hematopoietic system is an example of its application. SCS has not only assisted in the construction of relevant hematopoietic cell profiles under physiological and pathophysiological conditions in basic research

but also explored possible pathways of hematopoietic cell development and differentiation in clinical practice. Moreover

in the process of clinical diagnosis and treatment

it can effectively promote the deciphering of mechanisms of disease occurrence

progression and prognosis

and facilitate the exploration of potential factors of phenomena such as significant differences in individual outcomes and ease of relapse. Therefore

this paper reviews the technical overview and development trend of SCS and its application in the hematopoietic system

with a view to providing references for subsequent positive and reasonable application of SCS.

关键词

Keywords

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