烟草烟雾提取物激活NLRP3炎性小体促进肺腺癌PC-9细胞侵袭

李久康; 陈虹

doi:10.16016/j.1000-5404.202104196

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烟草烟雾提取物激活NLRP3炎性小体促进肺腺癌PC-9细胞侵袭

基础医学 | 更新时间：2026-01-09

- 烟草烟雾提取物激活NLRP3炎性小体促进肺腺癌PC-9细胞侵袭
- Cigarette smoke extract activates NLRP3 inflammasome to promote invasion of lung adenocarcinoma PC-9 cells
- 第三军医大学学报 2021年43卷第20期页码：2214-2219
- 作者机构：
  
  400016 重庆，重庆医科大学附属第一医院呼吸与危重症医学科
- 作者简介：
  
  [ 陈虹, E-mail: hopehong2003@126.com CHEN Hong, E-mail: hopehong2003@126.com
- 基金信息：
- DOI：10.16016/j.1000-5404.202104196
  中图分类号： R363.13;R730.23;R734.2
- 收稿：2021-04-29，
  
  修回：2021-07-05，
  
  纸质出版：2021-10-30
- 稿件说明：
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李久康, 陈虹. 烟草烟雾提取物激活NLRP3炎性小体促进肺腺癌PC-9细胞侵袭[J]. 第三军医大学学报, 2021,43(20):2214-2219.

Jiukang LI, Hong CHEN. Cigarette smoke extract activates NLRP3 inflammasome to promote invasion of lung adenocarcinoma PC-9 cells[J]. Journal of Third Military Medical University, 2021, 43(20): 2214-2219.
李久康, 陈虹. 烟草烟雾提取物激活NLRP3炎性小体促进肺腺癌PC-9细胞侵袭[J]. 第三军医大学学报, 2021,43(20):2214-2219. DOI： 10.16016/j.1000-5404.202104196.

Jiukang LI, Hong CHEN. Cigarette smoke extract activates NLRP3 inflammasome to promote invasion of lung adenocarcinoma PC-9 cells[J]. Journal of Third Military Medical University, 2021, 43(20): 2214-2219. DOI： 10.16016/j.1000-5404.202104196.

摘要

目的

探讨烟草烟雾(cigarette smoke extract

CSE)提取物对肺腺癌PC-9细胞侵袭能力的影响及机制。

方法

体外培养PC-9细胞，与0%、1%、2%、4%烟草烟雾提取物共培养96 h。Transwell实验检测细胞侵袭能力，Western blot检测NLRP3、Caspase-1、IL-1β及MMP-2蛋白表达。予以4%烟草烟雾提取物与MCC950 (NLRP3抑制剂)共同处理96 h后，细胞侵袭实验检测细胞侵袭能力，Western blot检测NLRP3、Caspase-1、IL-1β及MMP-2蛋白表达。

结果

与对照组相比，烟草提取物处理组中发生侵袭的PC-9细胞数量呈浓度(1%~4% CSE)依耐性增多(

0.05，

0.01)。NLRP3、Caspase-1、IL-1β及MMP-2的蛋白水平在烟草提取物处理组中呈浓度依耐性上调(

0.01)。MCC950干预后，可显著抑制烟草烟雾提取物诱导的PC-9侵袭细胞的增多(

0.05)以及NLRP3、Caspase-1、IL-1β、MMP-2表达的上调(

0.05)。

结论

烟草烟雾提取物可增强肺腺癌细胞侵袭能力，可能与激活NLRP3炎性小体有关。

Abstract

Objective

To investigate the effect and underlying mechanism of cigarette smoke extract (CSE) on invasion ability of lung adenocarcinoma PC-9 cells.

Methods

PC-9 cells were cultured in the medium containing 0%

2% and 4% tobacco smoke extract for 96 h

respectively. The invasive ability of the cells was detected by Transwell assay. The protein levels of NLRP3

Caspase-1

IL-1β and MMP-2 were measured with Western blotting. After co-treatment with 4% CSE and NLRP3 inhibitor

MCC950

for 96 h

Transwell assay was employed again for the invasive ability of the cells

and Western blotting for the expression of NLRP3

Caspase-1

IL-1β and MMP-2.

Results

CSE significantly increased the invasive ability of PC-9 cells (

0.05

0.01) and promoted the protein expression of NLRP3

Caspase-1

IL-1β and MMP-2(

0.01) in dose-dependent manners. The intervention of MCC950 obviously inhibited the enhanced invasive ability of PC-9 cells and the up-regulation of NLRP3

Caspase-1

IL-1β and MMP-2 induced by CSE.

Conclusion

CSE enhances the invasive ability of lung adenocarcinoma cells

which may be associated with the activation of NLRP3 inflammasome.

关键词

Keywords

references

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