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hsa_circ_0001708与IKZF1的比值在儿童急性B淋巴细胞白血病诊断及分型中的价值
Clinical significance of diagnosis and classification of ratio of hsa a_circ_0001708 to IKZF1 for pediatric B-ALI
- 第三军医大学学报 2021年43卷第20期 页码：2173-2179
- 作者机构：
1.400014 重庆，重庆医科大学附属儿童医院临床分子医学中心
2.625000 四川 雅安，雅安市人民医院检验科
3.610000 成都，成都市第三人民医院实验医学科临床检验部
4.200062 上海，上海交通大学附属儿童医院临床研究中心
- 作者简介：
[ 邹琳, E-mail: zoulin74@126.com ZOU Lin, E-mail: zoulin74@126.com
- 基金信息：国家自然科学基金面上项目(81570142)
DOI：10.16016/j.1000-5404.202104159
中图分类号： R394.2;R730.43;R733.7收稿：2021-04-24，
修回：2021-07-04，
纸质出版：2021-10-30
- 稿件说明：
移动端阅览
付立芳, 何霞, 李佳, 等. hsa_circ_0001708与IKZF1的比值在儿童急性B淋巴细胞白血病诊断及分型中的价值[J]. 第三军医大学学报, 2021,43(20):2173-2179.
Lifang FU, Xia HE, Jia LI, et al. Clinical significance of diagnosis and classification of ratio of hsa a_circ_0001708 to IKZF1 for pediatric B-ALI[J]. Journal of Third Military Medical University, 2021, 43(20): 2173-2179.
付立芳, 何霞, 李佳, 等. hsa_circ_0001708与IKZF1的比值在儿童急性B淋巴细胞白血病诊断及分型中的价值[J]. 第三军医大学学报, 2021,43(20):2173-2179. DOI： 10.16016/j.1000-5404.202104159.
Lifang FU, Xia HE, Jia LI, et al. Clinical significance of diagnosis and classification of ratio of hsa a_circ_0001708 to IKZF1 for pediatric B-ALI[J]. Journal of Third Military Medical University, 2021, 43(20): 2173-2179. DOI： 10.16016/j.1000-5404.202104159.
摘要
目的
2
探讨hsa_circ_0001708与IKZF1在儿童急性B淋巴细胞白血病（B-cell acute lymphoblastic leukemia
B-ALL）骨髓单个核细胞（bone marrow mononuclear cells
BMNC）中的拷贝数比值及其在儿童B-ALL诊断及分型中的临床意义。
方法
2
利用T-A克隆构建包含hsa_circ_0001708反向剪接位点的重组质粒，梯度稀释重组质粒作为标准品建立检测hsa_circ_0001708的绝对RT-qPCR方法。收集2016年1月至2019年1月重庆医科大学附属儿童医院血液科初诊的159例B-ALL患儿骨髓标本为研究对象。对照组为同期就诊的17例非恶性血液病患儿的骨髓标本。采用绝对RT-qPCR方法检测B-ALL患儿和对照患儿BMNC中hsa_circ_0001708和IKZF1的绝对拷贝数，按患者年龄、性别、外周血白细胞数、分子生物学、遗传学、免疫分型、随访期是否复发及随访期末是否生存等临床特点进行分组。回顾性分析hsa_circ_0001708和IKZF1的比值在各组中的表达情况及与以上各因素的相关性。
结果
2
建立精确定量hsa_circ_0001708拷贝数的绝对RT-qPCR方法。与非血液肿瘤对照患儿相比，hsa_circ_0001708与IKZF1的比值在儿童B-ALL中升高（
P
<
0.01），ROC曲线下面积为0.793 6。相关性分析结果提示，hsa_circ_0001708与IKZF1的比值在外周血高白细胞数组、外周血高原始细胞数组、免疫分型pre-B组和细胞遗传学预后较差组中升高（
P
<
0.05）。
结论
2
hsa_circ_0001708与IKZF1的比值对儿童B-ALL有潜在诊断价值，且与B-ALL患儿的MIC分型密切相关。
Abstract
Objective
2
To investigate the clinical significance of the ratio of hsa_circ_0001708 to IKZF1 in bone marrow mononuclear cells (BMNC) in pediatric B-cell acute lymphoblastic leukemia (B-ALL).
Methods
2
T-A clone was used to construct the recombinant plasmid containing the back-spliced junction of hsa_circ_0001708
regarding the gradient diluted recombinant plasmid as standard substance to establish the absolute fluorescence quantitative PCR (qPCR) for the detection of hsa_circ_0001708. Bone marrow samples from 159 newly diagnosed pediatric B-ALL patients and 17 patients with non-malignant hemotologic diseases in their matched control group admitted in the Children's Hospital of Chongqing Medical University from January 2016 to January 2019. The copy number of hsa_circ_0001708 and IKZF1 in BMNC of B-ALL children were determined by absolute fluorescence qPCR. The patients were divided into different groups according to their age
gender
peripheral blood leukocyte count
molecular biology
genetics
immune classification
and whether relapse and survive during the follow-up period. The ratio of hsa_circ_0001708 to IKZF1 in different groups and the correlation with these clinical characteristics were analyzed retrospectively.
Results
2
The absolute RT-qPCR was established that can accurately quantify the copy number of hsa_circ_0001708. The ratio of hsa_circ_0001708 to IKZF1 was significantly increased in pediatric B-ALL (
P
<
0.01) when compared with the controls
and the area under the ROC curve (AUC) was 0.793 6. The correlation analysis indicated that the ratio was significant increased in these groups of high leukocyte count
high blast cell count of peripheral blood
pre-B and poor prognosis in cytogenetics (
P
<
0.05).
Conclusion
2
The ratio of hsa_circ_0001708 to IKZF1 has potential diagnostic value and is related to the MIC classification for pediatric B-ALL.
关键词
Keywords
references
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