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鲍曼不动杆菌快速表型药敏检测方法的建立和初步应用
Establishment and preliminary application of a rapid pathogen-specific phenotypic antibiotic susceptibility test for
Acinetobacter baumannii - 第三军医大学学报 2021年43卷第18期 页码：1775-1782
- 作者机构：
400038 重庆，陆军军医大学(第三军医大学)第一附属医院药剂科
- 作者简介：
[枉前，E-mail: xqwq411@126.com WANG Qian, E-mail: xqwq411@126.com
- 基金信息：国家自然科学基金青年科学基金(81802117);陆军军医大学优秀青年人才库(XZ-2019-505-012)
DOI：10.16016/j.1000-5404.202104143
中图分类号： R378.7;R446.5;R915收稿：2021-04-22，
修回：2021-06-30，
纸质出版：2021-09-30
- 稿件说明：
移动端阅览
罗丹, 李玉良, 翁邦碧, 等. 鲍曼不动杆菌快速表型药敏检测方法的建立和初步应用[J]. 第三军医大学学报, 2021,43(18):1775-1782.
Dan LUO, Yuliang LI, Bangbi WENG, et al. Establishment and preliminary application of a rapid pathogen-specific phenotypic antibiotic susceptibility test for
Acinetobacter baumannii [J]. Journal of Third Military Medical University, 2021, 43(18): 1775-1782.罗丹, 李玉良, 翁邦碧, 等. 鲍曼不动杆菌快速表型药敏检测方法的建立和初步应用[J]. 第三军医大学学报, 2021,43(18):1775-1782. DOI： 10.16016/j.1000-5404.202104143.
Dan LUO, Yuliang LI, Bangbi WENG, et al. Establishment and preliminary application of a rapid pathogen-specific phenotypic antibiotic susceptibility test for
Acinetobacter baumannii [J]. Journal of Third Military Medical University, 2021, 43(18): 1775-1782. DOI： 10.16016/j.1000-5404.202104143.
摘要
目的
2
利用表面活性剂-叠氮溴化丙锭(propidium monoazide
PMAxx)-qPCR反应体系对鲍曼不动杆菌进行快速表型药敏预测。
方法
2
依次对加入反应体系中表面活性剂的种类和最适浓度、叠氮溴化丙锭曝光时间和浓度进行优化；采用qPCR检测鲍曼不动杆菌特异性鉴别基因(blaOXA-51)拷贝数；采用抗菌药物-细菌共反应体系测定鲍曼不动杆菌对抗菌药物的敏感性。
结果
2
当表面活性剂月桂酰基甘氨酸钠浓度为0.2%，叠氮溴化丙锭最佳曝光时间和浓度为5 min和20 μmol/L时，能有效抑制死细胞DNA扩增，且不影响活细胞。基于此建立的表型药敏预测模型能快速(2 h内)和准确地预测鲍曼不动杆菌对多西环素及左氧氟沙星的敏感性(
P
<
0.01)，对头孢哌酮/舒巴坦的敏感性更可靠的预测需超过2 h。
结论
2
基于月桂酰基甘氨酸钠-PMAxx-qPCR体系的快速表型药敏预测新方法可在短时间内为鲍曼不动杆菌感染的治疗提供药敏依据，以指导其临床精准用药。
Abstract
Objective
2
To establish a rapid phenotypic antibiotic susceptibility testing of
Acinetobacter baumannii
based on surfactant-propidium monoazide (PMAxx)-qPCR reaction system.
Methods
2
The type and optimal concentration of surfactant
exposure time and concentration of PMAxx were optimized successively. The copies of genomic DNA of
Acinetobacter baumannii
specific discriminant gene (blaOXA-51) was detected by qPCR. The susceptibility of
Acinetobacter baumannii
to antibiotics was determined by the antibiotic-bacterial co-reaction system.
Results
2
The Results indicated that 0.2% sodium lauroylsarcosinate and 20 μmol/L PMAxx af
ter 5 min exposure effectively inhibited the DNA amplification of dead cells but had no effect on that of live cells. The phenotypic antibiotic susceptibility testing model based on this study could quickly (within 2 h) and accurately predict the susceptibility of
Acinetobacter baumannii
to doxycycline and levofloxacin (P
<
0.01)
and the reliable susceptibility of cefoperazone/sulbactam was predicted more than 2 h.
Conclusion
2
Our phenotypic antibiotic susceptibility test based on the sodium lauroylsarcosinate-PMAxx-qPCR system provides a basis for the treatment of
Acinetobacter baumannii
within a short time
and guides the clinical precision medication.
关键词
Keywords
references
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