槲皮素通过MAPK/ERK1/2信号途径对H9C2细胞抗缺氧损伤的作用

何依津; 张鹏志; 田海月; 李静; 王育花

doi:10.16016/j.1000-5404.202104138

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槲皮素通过MAPK/ERK1/2信号途径对H9C2细胞抗缺氧损伤的作用

基础医学 | 更新时间：2026-01-09

- 槲皮素通过MAPK/ERK1/2信号途径对H9C2细胞抗缺氧损伤的作用
- Quercetin protects H9C2 cells against hypoxia injury through MAPK/ERK1/2 signal pathway
- 第三军医大学学报 2021年43卷第20期页码：2220-2225
- 作者机构：
  
  730050 兰州，中国人民解放军联勤保障部队第九四〇医院耳鼻咽喉头颈外科
- 作者简介：
  
  [ 王育花，E-mail：945519871@qq.com WANG Yuhua, E-mail: 945519871@qq.com
- 基金信息：
  
  甘肃省青年科技基金计划(20JR10RA011)
- DOI：10.16016/j.1000-5404.202104138
  中图分类号： R282.71;R322.11;R594.3
- 收稿：2021-04-21，
  
  修回：2021-05-29，
  
  纸质出版：2021-10-30
- 稿件说明：
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何依津, 张鹏志, 田海月, 等. 槲皮素通过MAPK/ERK1/2信号途径对H9C2细胞抗缺氧损伤的作用[J]. 第三军医大学学报, 2021,43(20):2220-2225.

Yijin HE, Pengzhi ZHANG, Haiyue TIAN, et al. Quercetin protects H9C2 cells against hypoxia injury through MAPK/ERK1/2 signal pathway[J]. Journal of Third Military Medical University, 2021, 43(20): 2220-2225.
何依津, 张鹏志, 田海月, 等. 槲皮素通过MAPK/ERK1/2信号途径对H9C2细胞抗缺氧损伤的作用[J]. 第三军医大学学报, 2021,43(20):2220-2225. DOI： 10.16016/j.1000-5404.202104138.

Yijin HE, Pengzhi ZHANG, Haiyue TIAN, et al. Quercetin protects H9C2 cells against hypoxia injury through MAPK/ERK1/2 signal pathway[J]. Journal of Third Military Medical University, 2021, 43(20): 2220-2225. DOI： 10.16016/j.1000-5404.202104138.

摘要

目的

研究槲皮素对H9C2心肌细胞抗缺氧损伤的作用及机制。

方法

建立H9C2细胞缺氧模型，依次测定乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)和丙二醛(MDA)的含量，使用MTT法获得槲皮素作用H9C2细胞最适宜药物浓度。根据H9C2细胞不同处理分为对照组(C)、缺氧模型组(H)、SCH772984组(S)、槲皮素组(Q)、槲皮素+缺氧模型组(Q+H)和槲皮素+SCH772984组(Q+S)。各组处理完成后再依次检测LDH、SOD和MDA的含量，并观察MAPK、ERK1/2蛋白及其磷酸化后的表达水平。

结果

H9C2细胞对缺氧培养条件具有一定时间的依赖性，缺氧培养24 h后出现显著的缺氧损伤，LDH和MDA水平显著增加而SOD水平显著降低(

0.05)；MTT检测结果显示10

-6

mol/L浓度的槲皮素显著促进H9C2细胞增殖(

0.05)。10

-6

mol/L槲皮素显著降低Q组和S组H9C2细胞培养中LDH和MDA的含量(

0.05)，并显著提高SOD的含量(

0.05)。10

-6

mol/L浓度的槲皮素也提高了H9C2细胞p-MAPK和p-ERK1/2磷酸化水平。

结论

槲皮素能有效抵抗缺氧引起的H9C2细胞损伤，提高细胞抗氧化能力，清除缺氧过程中产生的有害物质，激活MAPK/ERK1/2信号途径发挥抗缺氧化对H9C2细胞损伤的作用。

Abstract

Objective

To investigate the effect and mechanism of quercetin on H9C2 cells against hypoxia injury.

Methods

The contents of lactate dehydrogenase (LDH)

superoxide dismutase (SOD) and malondialdehyde (MDA) were determined in a hypoxia model of H9C2 cells. MTT assay was used to measure the optimal concentration of quercetin to H9C2 cells. Then the H9C2 cells were divided into control group (C)

hypoxia group (H)

SCH772984 group (S)

quercetin group (Q)

quercetin+hypoxia group (Q+H)

and quercetin+SCH772984 group (Q+S). After the treatment of each group was completed

the contents of LDH

SOD and MDA were successively detected

and the expression levels of MAPK

ERK1/2 proteins and their phosphorylation were observed.

Results

H9C2 cells were dependent on hypoxia culture conditions for a certain period of time. After 24 h of hypoxia culture

significant hypoxic damaged were observed. LDH and MDA contents were increased significantly while that of SOD was decreased significantly (

0.05). The results of MTT assay showed quercetin at a concentration of 10

-6

mol/L significantly promoted the proliferation of H9C2 cells (

0.05)

reduced the contents of LDH and MDA in the H9C2 cell of the Q and S groups (

0.05)

and significantly increased the content of SOD (

0.05). In addition

the concentration also increased the phosphorylation levels of p-MAPK and p-ERK1/2 in H9C2 cells.

Conclusion

Quercetin can effectively resist the damage of H9C2 cells caused by hypoxia

improve antioxidant capacity

remove harmful substances produced during hypoxia

and activate the MAPK/ERK1/2 signal pathway to play an anti-hypoxic effect.

关键词

Keywords

references

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