冠蛋白-1通过调控CD14的表达影响巨噬细胞极化

范晓霞; 陈全; 孙雪花; 田若源

doi:10.16016/j.1000-5404.202104087

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冠蛋白-1通过调控CD14的表达影响巨噬细胞极化

基础医学 | 更新时间：2026-01-09

- 冠蛋白-1通过调控CD14的表达影响巨噬细胞极化
- Effect of Coronin-1 on macrophage polarization by regulating the expression of CD14
- 第三军医大学学报 2021年43卷第19期页码：1884-1893
- 作者机构：
  
  400016 重庆，重庆医科大学基础医学院免疫学教研室
- 作者简介：
  
  [陈全，E-mail: chenquan@cqmu.edu.cn CHEN Quan, E-mail: chenquan@cqmu.edu.cn
- 基金信息：
- DOI：10.16016/j.1000-5404.202104087
  中图分类号： R341;R392.12;R392.2
- 收稿：2021-04-14，
  
  修回：2021-06-07，
  
  纸质出版：2021-10-15
- 稿件说明：
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范晓霞, 陈全, 孙雪花, 等. 冠蛋白-1通过调控CD14的表达影响巨噬细胞极化[J]. 第三军医大学学报, 2021,43(19):1884-1893.

Xiaoxia FAN, Quan CHEN, Xuehua SUN, et al. Effect of Coronin-1 on macrophage polarization by regulating the expression of CD14[J]. Journal of Third Military Medical University, 2021, 43(19): 1884-1893.
范晓霞, 陈全, 孙雪花, 等. 冠蛋白-1通过调控CD14的表达影响巨噬细胞极化[J]. 第三军医大学学报, 2021,43(19):1884-1893. DOI： 10.16016/j.1000-5404.202104087.

Xiaoxia FAN, Quan CHEN, Xuehua SUN, et al. Effect of Coronin-1 on macrophage polarization by regulating the expression of CD14[J]. Journal of Third Military Medical University, 2021, 43(19): 1884-1893. DOI： 10.16016/j.1000-5404.202104087.

摘要

目的

探讨冠蛋白-1(Coronin-1)与巨噬细胞极化的相关性及可能机制。

方法

建立M1、M2型RAW264.7鼠巨噬细胞模型，通过Real-time PCR和Western blot检测Coronin-1的表达；构建过表达(Coronin-1 Plus)、敲除(Coronin-1 KO)细胞系，通过Real-time PCR和Western blot检测过表达、敲除和正常表达Coronin-1细胞组的一氧化氮合酶(inducible nitric oxide synthase

iNOS)水平；利用蛋白质互作网络(PPI)和GO分析Coronin-1和CD14相关蛋白的互作与富集，再通过Real-time PCR和Western blot检测过表达、敲除和正常表达Coronin-1细胞组的CD14水平以及经脂多糖(lipopolysaccharide

LPS，100 ng/mL)处理后的iNOS水平；构建CD14敲减的细胞系(Control-CD14 KD)、CD14敲减的Coronin-1 Plus细胞系(Coronin-1 Plus-CD14 KD)，经LPS (100 ng/mL)处理后，通过Real-time PCR和Western blot检测iNOS的表达。

结果

成功构建M1、M2型巨噬细胞模型，M1型Coronin-1表达显著高于对照组(

0.05)；Coronin-1 Plus组iNOS mRNA的表达显著低于对照组(

0.01)，Coronin-1 Plus组CD14的表达显著高于对照组(

0.05)；经LPS处理后，Coronin-1 Plus组iNOS的表达显著高于对照组(

0.01)；经LPS处理的Control-CD14 KD、Coronin-1 Plus-CD14 KD组细胞的iNOS水平与对照组比较，差异无统计学意义。

结论

巨噬细胞向M1型极化导致了Coronin-1的高表达，而Coronin-1过表达并不能直接导致细胞向M1型极化，但Coronin-1过表达可导致CD14表达上调，Coronin-1可通过CD14途径促进LPS诱导巨噬细胞向M1型极化。

Abstract

Objective

To investigate the correlation between Coronin-1 and macrophage polarization and its possible mechanism.

Methods

Firstly

M1 and M2 RAW246.7 macrophage cells were established

and the expression of Coronin-1 at mRNA and protein level was detected by real-time PCR and Western blotting. Then

over-expressed (Coronin-1 Plus) and knockout (Coronin-1 KO) cell lines were constructed in RAW246.7 cells

and the expression levels of inducible nitric oxide synthase (iNOS) in over-expressed

knockout and normal-expressed Coronin-1 cells were detected by real-time PCR and Western blotting. The interaction and enrichment of Coronin-1 and CD14 related proteins were analyzed by protein-protein interaction (PPI) network and Gene Ontology (GO) enrichment analysis. Real-time PCR and Western blotting were used to detect the expression level of CD14 in over-expressed

knockout and normal Coronin-1 cells

and the expression level of iNOS after treatment with 100 ng/mL LPS. Then

CD14 knockdown cell lines (Control-CD14 KD) and CD14 knockdown Coronin-1 Plus cell lines (Coronin-1 Plus-CD14 KD) were constructed. After treatment with LPS (100 ng/mL)

the expression of iNOS was detected by real-time PCR and Western blotting.

Results

M1 and M2 macrophage models were successfully constructed

and the expression of Coronin-1 in M1 type macrophages was significantly higher than that in the Control group (

0.05). The mRNA expression of iNOS was significantly lower in Coronin-1 Plus group than control group (

0.01)

and the expression of CD14 was obviously higher in Coronin-1 Plus group than Control group (

0.05). After LPS treatment

the expression of iNOS in Coronin-1 Plus group was significantly higher than that in Control group (

0.01). There was no significant difference in iNOS level of the LPS treated Control-CD14 KD and Coronin-1 Plus-CD14 KD groups with the Control group (

0.05).

Conclusion

The M1 polarization of macrophages leads to higher expression of Coronin-1

but the over-expression of Coronin-1 does not directly lead to the M1 polarization of macrophages. Its over-expression can lead to up-regulation of CD14 expression

and Coronin-1 can promote LPS-induced macrophages to M1 type polarization through the CD14 pathway.

关键词

Keywords

references

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