MiR-138通过靶向SIRT1调控结肠癌细胞的凋亡

杨洋; 彭文丽

doi:10.16016/j.1000-5404.202104063

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MiR-138通过靶向SIRT1调控结肠癌细胞的凋亡

基础医学 | 更新时间：2026-01-09

- MiR-138通过靶向SIRT1调控结肠癌细胞的凋亡
- MiR-138 promotes apoptosis of colon cancer cells by targeting SIRT1
- 第三军医大学学报 2021年43卷第19期页码：1877-1883
- 作者机构：
  
  400030 重庆，重庆大学附属肿瘤医院，肿瘤转移与个体化诊治转化研究重庆市重点实验室
- 作者简介：
  
  [彭文丽，E-mail: 155059627@qq.com PENG Wenli, E-mail: 155059627@qq.com
- 基金信息：
- DOI：10.16016/j.1000-5404.202104063
  中图分类号： R394.3;R73-362;R735.35
- 收稿：2021-04-10，
  
  修回：2021-07-08，
  
  纸质出版：2021-10-15
- 稿件说明：
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杨洋, 彭文丽. MiR-138通过靶向SIRT1调控结肠癌细胞的凋亡[J]. 第三军医大学学报, 2021,43(19):1877-1883.

Yang YANG, Wenli PENG. MiR-138 promotes apoptosis of colon cancer cells by targeting SIRT1[J]. Journal of Third Military Medical University, 2021, 43(19): 1877-1883.
杨洋, 彭文丽. MiR-138通过靶向SIRT1调控结肠癌细胞的凋亡[J]. 第三军医大学学报, 2021,43(19):1877-1883. DOI： 10.16016/j.1000-5404.202104063.

Yang YANG, Wenli PENG. MiR-138 promotes apoptosis of colon cancer cells by targeting SIRT1[J]. Journal of Third Military Medical University, 2021, 43(19): 1877-1883. DOI： 10.16016/j.1000-5404.202104063.

摘要

目的

探讨miR-138在结肠癌细胞中通过调控沉默信息调节因子2相关酶1(silent mating type information regulation 2 homolog-1，SIRT1)参与细胞凋亡的机制。

方法

采用RT-qPCR检测miR-138在结肠癌细胞株HCT116、SW480、Caco-2、HT29、SW1116中的表达。构建荧光素酶报告质粒，双荧光素酶实验验证靶标分子。将miR-138 mimics(类似物)、miR-138 inhibitors(抑制物)和miR-138 NC(阴性对照)分别转染至HT29、Caco-2细胞，RT-qPCR和Western bolt检测处理前后HCT116细胞中miR-138、SIRT1、Caspase-3及Bcl-2的表达；流式细胞仪检测HT29、Caco-2细胞凋亡情况。

结果

miR-138在结肠癌细胞Caco-2中表达水平最低，在HT29细胞中表达水平最高。双荧光素酶实验验证结果显示: 在HT29细胞中，SIRT1为miR-138的靶标分子，miR-138可在mRNA及蛋白水平抑制SIRT1的表达。miR-138 mimics转染至Caco-2细胞后，其SIRT1、Bcl-2表达显著降低(

0.05)，Caspase-3表达显著升高(

0.05)，细胞凋亡增加(

0.05)；而miR-138 inhibitors转染至HT29细胞后，其SIRT1、Bcl-2表达显著升高(

0.05)，Caspase-3表达显著降低(

0.05)，细胞凋亡减少(

0.05)。

结论

miR-138可通过影响其靶标分子SIRT1的表达进而参与结肠癌细胞凋亡水平的调控。

Abstract

Objective

To investigate the mechanism of miR-138 involving in the apoptosis of colon cancer cells by regulating SIRT1.

Methods

RT-qPCR was used to detect the expression of miR-138 in colon cancer cell lines HCT 116

SW480

Caco-2

HT29 and SW1116. The luciferase reporter plasmid was constructed and the target molecule was verified by double luciferase assay. MiR-138 mimics

miR-138 inhibitor and miR-138 NC (negative control) were transfected into HT29 and Caco-2 cells

respectively. The expression levels of miR-138

SIRT1

Caspase-3 and Bcl-2 in the cells of each group were detected by RT-qPCR and Western blotting

and the apoptosis of Caco-2 and HT29 cells was detected by flow cytometry.

Results

MiR-138 showed the lowest expression level in Caco-2 cells and the highest expression level in HT29 cells. SIRT1 was identified as a direct target of miR-138 in HT29 cells

and miR-138 inhibited SIRT1 expression at both mRNA and protein levels. After transfection of miR-138 mimics into Caco-2 cells

the expression of SIRT1 and Bcl-2 was significantly dec

reased (

0.05)

Caspase-3 was enhanced (

0.05)

meanwhile the apoptosis was increased (

0.05). Whereas after miR-138 inhibitor transfected into HT29 cells

the expression of SIRT1 and Bcl-2 was obviously increased (

0.05)

Caspase-3 was decreased (

0.05)

and the apoptosis reduced (

0.05).

Conclusion

MiR-138 promotes apoptosis of colon cancer cells by regulating the expression of its target SIRT1.

关键词

Keywords

references

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