当归多糖拮抗D-半乳糖致大鼠脑衰老的作用

汪兰; 张梦思; 王顺和; 汪子铃; 侯吉颖; 肖含先之; 黄彩虹; 曾娣; 张进; 牛雪芳; 牛依琳; 王璐; 王亚平

doi:10.16016/j.1000-5404.202103187

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当归多糖拮抗D-半乳糖致大鼠脑衰老的作用

神经科学 | 更新时间：2026-01-09

- 当归多糖拮抗D-半乳糖致大鼠脑衰老的作用
- Angelica sinensis polysaccharide antagonizes D-galactose induced brain aging in rats
- 第三军医大学学报 2021年43卷第19期页码：1839-1845
- 作者机构：
  
  1.400016 重庆，重庆医科大学基础医学院: 组织学与胚胎学教研室，干细胞与组织工程研究室
  2.400016 重庆，重庆医科大学基础医学院: 病理学教研室
- 作者简介：
  
  [王亚平，E-mail: ypwangcq@aliyun.com WANG Yaping, E-mail: ypwangcq@aliyun.com
- 基金信息：
  
  国家自然科学基金面上项目(81673748);国家自然科学基金面上项目(81873103)
- DOI：10.16016/j.1000-5404.202103187
  中图分类号： R285.5;R338.64;R339.38
- 收稿：2021-03-22，
  
  修回：2021-05-10，
  
  纸质出版：2021-10-15
- 稿件说明：
移动端阅览
汪兰, 张梦思, 王顺和, 等. 当归多糖拮抗D-半乳糖致大鼠脑衰老的作用[J]. 第三军医大学学报, 2021,43(19):1839-1845.

Lan WANG, Mengsi ZHANG, Shunhe WANG, et al. Angelica sinensis polysaccharide antagonizes D-galactose induced brain aging in rats[J]. Journal of Third Military Medical University, 2021, 43(19): 1839-1845.
汪兰, 张梦思, 王顺和, 等. 当归多糖拮抗D-半乳糖致大鼠脑衰老的作用[J]. 第三军医大学学报, 2021,43(19):1839-1845. DOI： 10.16016/j.1000-5404.202103187.

Lan WANG, Mengsi ZHANG, Shunhe WANG, et al. Angelica sinensis polysaccharide antagonizes D-galactose induced brain aging in rats[J]. Journal of Third Military Medical University, 2021, 43(19): 1839-1845. DOI： 10.16016/j.1000-5404.202103187.

摘要

目的

探讨当归多糖(

Angelica sinensis

polysaccharide，ASP)拮抗氧化致衰剂D-半乳糖(D-galactose，D-gal)致大鼠脑衰老的作用。

方法

将SD大鼠分为对照组、ASP组、衰老模型组、ASP衰老模型组。采用水迷宫检测各组大鼠空间学习记忆能力；衰老相关β半乳糖苷酶(SA-β-Gal)染色检测脑组织细胞衰老；色谱法测量海马匀浆SOD、MDA和GSH的含量；ELISA法检测海马组织匀浆中IL-1β，IL-6的含量；Southern blot检测海马细胞端粒长度，TRAP-PCR检测端粒酶活性。从SD胎鼠脑组织中分离、培养、鉴定神经干细胞(neural stem cells，NSCs)。将培养第3代NSCs分为对照组、ASP组、衰老模型组、ASP衰老模型组。色谱法测定细胞内MDA含量；流式细胞仪测定细胞内ROS水平；RT-PCR检测衰老相关基因的p19、p21、p53 mRNA的表达。

结果

与衰老模型组相比，ASP衰老模型组大鼠空间学习记忆能力明显改善；脑组织中SA-β-Gal染色阳性的细胞数减少(

0.05)；海马区SOD、GSH含量增高，MDA含量降低(

0.05)；IL-1β，IL-6的分泌水平降低(

0.05)；端粒长度和端粒酶活性增加。成功分离并鉴定出NSCs；与NSCs衰老组相比，ASP衰老组NSCs中MDA含量和ROS水平显著降低(

0.05)；衰老相关基因p19、p21、p53 mRNA表达降低(

0.05)。

结论

ASP可能通过调控细胞端粒长度和端粒酶活性，下调衰老相关基因表达，降低炎症因子表达，提高细胞抗氧化能力，进而拮抗D-gal对NSCs和海马细胞的致衰作用。

Abstract

Objective

To explore the antagonistic effect of

Angelica sinensis polysaccharide

(ASP) on brain aging induced by D-galactose (D-gal) in rats.

Methods

SD rats were randomly divided into control group

ASP group

aging model group

and ASP aging model group. Their spatial learning and memory abilities were detected by water maze test. The aging of brain tissue cells were detectded by aging-related β-galactosidase (SA-β-Gal) staining. The contents of SOD

MDA and GSH in hippocampal homogenate were measured by chromatography

and those of IL-1β and IL-6 in hippocampal homogenate were detected by ELISA. Southern blotting and TRAP-PCR were used to detect telomere length and telomerase activity in the hippocampal cells. Neural stem cells (NSCs) were isolated

cultured and identified from the brain tissue of SD fetal rats. The third-generation NSCs were divided into control group

ASP group

aging model group

and ASP aging model group. The content of MDA in the cells was determined by chromatography. The intracellular ROS level was determined by flow cytometry. qRT-PCR was used to detect the mRNA expression of p19

p21 and p53 of senescence related genes.

Results

Compared with the aging model group

the injection of ASP significantly improved the spatial learning and memory abilities of aging rats. The number of SA-β-Gal positive cells in the brain tissue was decreased (

0.05). The contents of SOD and GSH in the hippocampus were increased

but that of MDA was decreased (

0.05). The secretion levels of IL-1β and IL-6 were decreased (

0.05). The telomere length and telomerase activity were increased (

0.05). NSCs were successfully isolated and identified. Compared with the aging group

the MDA content and ROS level were significantly reduced in the ASP aging group (

0.05)

and the mRNA levels of senescence-related genes p19

p21

and p53 were decreased (

0.05).

Conclusion

ASP may regulate cell telomere length and telomerase activity

down-regulate the expression of aging-related genes

reduce the expression of inflammatory factors

improve the cell's antioxidant capacity

and then antagonize the degeneration effect of D-gal on NSCs and hippocampal cells.

关键词

Keywords

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