核转录共抑制因子、转化生长因子β1、Smad4在重度宫腔粘连内膜组织中表达及临床意义

杨博萍; 郭建新; 王昊; 袁艺萍; 池余刚; 李力

doi:10.16016/j.1000-5404.202103150

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核转录共抑制因子、转化生长因子β1、Smad4在重度宫腔粘连内膜组织中表达及临床意义

基础医学 | 更新时间：2026-01-09

- 核转录共抑制因子、转化生长因子β1、Smad4在重度宫腔粘连内膜组织中表达及临床意义
- Expression and clinical significance of SnoN, TGF-β1 and Smad4 in endometrium of patients with severe intrauterine adhesion
- 第三军医大学学报 2021年43卷第17期页码：1667-1672
- 作者机构：
- 作者简介：
- 基金信息：
  
  重庆市自然科学基金面上项目(cstc2020jcyj-msxmX0403);重庆市妇幼保健院院内课题面上项目(2019YJMS03)
- DOI：10.16016/j.1000-5404.202103150
  中图分类号：
- 收稿：2021-03-18，
  
  纸质出版：2021-09-15
- 稿件说明：
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杨博萍, 郭建新, 王昊, 等. 核转录共抑制因子、转化生长因子β1、Smad4在重度宫腔粘连内膜组织中表达及临床意义[J]. 第三军医大学学报, 2021,43(17):1667-1672.

Boping YANG, Jianxin GUO, Hao WANG, et al. Expression and clinical significance of SnoN, TGF-β1 and Smad4 in endometrium of patients with severe intrauterine adhesion[J]. Journal of Third Military Medical University, 2021, 43(17): 1667-1672.
杨博萍, 郭建新, 王昊, 等. 核转录共抑制因子、转化生长因子β1、Smad4在重度宫腔粘连内膜组织中表达及临床意义[J]. 第三军医大学学报, 2021,43(17):1667-1672. DOI： 10.16016/j.1000-5404.202103150.

Boping YANG, Jianxin GUO, Hao WANG, et al. Expression and clinical significance of SnoN, TGF-β1 and Smad4 in endometrium of patients with severe intrauterine adhesion[J]. Journal of Third Military Medical University, 2021, 43(17): 1667-1672. DOI： 10.16016/j.1000-5404.202103150.

摘要

目的

探讨核转录共抑制因子(ski-related novel protein N，SnoN)在宫腔粘连(intrauterine adhesions，IUA)发病中的作用，及其与转化生长因子β1 (transforming growth factor-β1，TGF-β1)、Smad4关系。

方法

收集重度宫腔粘连(sIUA组)和宫腔无病变的子宫纵隔患者(对照组)宫内膜组织各20例，通过免疫组化、Western blot、RT-qPCR检测子宫内膜组织中SnoN、TGF-β1、Smad4的表达水平，并应用Spearman等级相关分析SnoN、TGF-β1、Smad4之间的相关性，探讨其与宫腔粘连发病的关系。

结果

sIUA组患者宫内膜组织中TGF-β1、Smad4表达水平明显高于对照组(

0.01)，而SnoN表达水平明显低于对照组(

0.01)。在重度宫腔粘连宫内膜组织中，TGF-β1与Smad4的蛋白表达水平呈正相关(

=0.468 9，

0.05)，而TGF-β1与SnoN的蛋白水平呈负相关(

=－0.483 5，

0.05)。

结论

SnoN可能通过TGF-β1/Smad4信号通路参与宫腔粘连的发病。

Abstract

Objective

To investigate the role of ski-related novel protein N (SnoN) in the pathogenesis of intrauterine adhesion (IUA) and its association with transforming growth factor-β1 (TGF-β1) and Smad4 in endometrial tissue in IUA patients.

Methods

Endometrial tissues of 20 severe IUA patients (sIUA group) and 20 healthy women with uterine septum (control group) were collected from our 2 departments during January 2019 and October 2020. Immunohistochemical assay

Western blotting and reverse transcription quantitative polymerase chain reaction (RT-qPCR) were used to detect the expression of SnoN

TGF-β1 and Smad4 in the endometrial tissue of the control and sIUA groups. The correlations of their expression levels in the endometrial tissues were analyzed with Spearman rank correlation analysis to investigate the association of the levels and IUA.

Results

The expression levels of TGF-β1 and Smad4 were significantly higher

while that of SnoN was obviously lower in the sIUA group than the control group (all

0.01). In the endometrial tissues from the sIUA group

the expression of Smad4 was positively correlated with that of TGF-β1 (

=0.468 9

0.05). However

the TGF-β1 expression was negatively correlated with that of SnoN (

=-0.483 5

0.05).

Conclusion

SnoN may be involved in the pathogenesis of IUA

via

the TGF-β1/Smad4 signal pathway.

关键词

Keywords

references

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