去卵巢小鼠股骨微结构的动态变化研究

邱宇; 赵月阳; 龙志敏; 汪克建; 徐凌; 贺桂琼

doi:10.16016/j.1000-5404.202103089

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去卵巢小鼠股骨微结构的动态变化研究

基础医学 | 更新时间：2026-01-09

- 去卵巢小鼠股骨微结构的动态变化研究
- Dynamic changes of femur microarchitecture in ovariectomized mice
- 第三军医大学学报 2021年43卷第17期页码：1627-1633
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81671257);重庆高校创新团队建设计划资助项目(CXTDG201602006)
- DOI：10.16016/j.1000-5404.202103089
  中图分类号：
- 收稿：2021-03-10，
  
  纸质出版：2021-09-15
- 稿件说明：
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邱宇, 赵月阳, 龙志敏, 等. 去卵巢小鼠股骨微结构的动态变化研究[J]. 第三军医大学学报, 2021,43(17):1627-1633.

Yu QIU, Yueyang ZHAO, Zhimin LONG, et al. Dynamic changes of femur microarchitecture in ovariectomized mice[J]. Journal of Third Military Medical University, 2021, 43(17): 1627-1633.
邱宇, 赵月阳, 龙志敏, 等. 去卵巢小鼠股骨微结构的动态变化研究[J]. 第三军医大学学报, 2021,43(17):1627-1633. DOI： 10.16016/j.1000-5404.202103089.

Yu QIU, Yueyang ZHAO, Zhimin LONG, et al. Dynamic changes of femur microarchitecture in ovariectomized mice[J]. Journal of Third Military Medical University, 2021, 43(17): 1627-1633. DOI： 10.16016/j.1000-5404.202103089.

摘要

目的

探究去卵巢骨质疏松小鼠术后不同时期股骨微结构的动态变化。

方法

8周龄C57雌性小鼠分为去卵巢组（ovariectomy，OVX）和假手术组（sham ovariectomy

Sham），并根据术后不同时间将每组小鼠又分为1周、1个月及3个月组，分别代表绝经早、中、晚期。称质量后取小鼠股骨进行HE染色、免疫组织化学染色及Micro-CT扫描分析，评估各组股骨远端松质骨、股骨中段皮质骨微结构及成骨相关蛋白水平变化。

结果

小鼠松质骨形态参数：与Sham组相比，OVX组术后1周骨微结构未见明显破坏，术后1~3个月骨小梁断裂，骨髓腔增大，CT骨参数除Tb.Th无明显变化外

0.05)，BV/TV，Tb.N和Tb.Sp均出现显著改变（

0.01）；Sham组中，直到术后3个月才出现较1周组骨量显著减少的表现（

0.01）；OVX各组中骨丢失情况随时间增加而加重（

0.05，

0.01）。Runx2表达水平与Micro-CT松质骨分析结果基本一致。皮质骨形态参数：各时段OVX组均未出现较Sham组的明显差异（P

0.05）。

结论

8周龄C57小鼠在OVX术后1周至1个月松质骨微结构明显受损，早于皮质骨变化，可作为骨质疏松病理机制和防治研究的重点阶段。

Abstract

Objective

To explore the dynamic changes of femur microstructure in ovariectomized osteoporosis mice at different stages.

Methods

Female C57 mice (8 weeks old) were randomly divided into sham-operation (Sham) and ovariectomy (OVX) groups. Each group was subdivided into 1-week

1-month and 3-month subgroups on the basis of different postoperative time

which representeing early

middle and late menopause

respectively. The femurs were extracted for HE staining

immunohistochemistry and micro-CT analysis

and the changes in the bone microstructure of cancellous bone in the distal femurs

cortical bone in the middle femurs and the levels of osteogenic associated proteins in each group were evaluated.

Results

Compared with the Sham group

the bone microstructure of the OVX group showed no significant destruction at 1 week after operation

trabecula fracture and bone marrow cavity enlargement from 1 month to 3 months after operation

and significant changes in CT bone parameters BV/TV

Tb.N and Tb.Sp (

0.01

0.001) except Tb.Th (P

0.05). In the Sham group

the bone mass was significantly reduced until 3 months after operation compared with that in the 1-week group (

0.01). Bone loss in the OVX groups was worsened in a time dependent manner (

0.05

0.01

0.001). The expression level of Runx2 by immunohistochemistry was consistent with

the results of micro-CT analysis. No significant differences were found in morphologic indices of cortical bone between the Sham subgroups and the OVX subgroups (P

0.05).

Conclusion

Trabecular bone microarchitecture is impaired progressively after OVX operation in 8-week-old C57 mice from 1 week to 1 month

prior to cortical bone. This period can be regarded as the key time for studies on pathogenesis and prevention and treatment of osteoporosis.

关键词

Keywords

references

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