不同致痒剂诱导建立大鼠瘙痒模型的效果分析

任梦雪; 吴广延; 高兴; 隋建峰; 李红丽

doi:10.16016/j.1000-5404.202103036

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不同致痒剂诱导建立大鼠瘙痒模型的效果分析

基础医学 | 更新时间：2026-01-09

- 不同致痒剂诱导建立大鼠瘙痒模型的效果分析
- Comparison of rat itch model induced by different pruritogens
- 第三军医大学学报 2021年43卷第16期页码：1527-1534
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)基础医学院: 组织胚胎学教研室
  2.400038 重庆，陆军军医大学(第三军医大学)基础医学院: 教学实验中心
- 作者简介：
  
  [李红丽，E-mail: lihongli@tmmu.edu.cnLI Hongli, E-mail: lihongli@tmmu.edu.cn
- 基金信息：
  
  重庆市自然科学基金面上项目(cstc2020jcyj-msxmX0391);the Science and Technology Innovation Capability Improvement Project of Army Medical University(2019XQN01);Transformation Pre-research project of Basic Medical College of Army Medical University(2019JCZX04)
- DOI：10.16016/j.1000-5404.202103036
  中图分类号： R-332;R758.31;R965.1
- 收稿：2021-03-03，
  
  修回：2021-06-04，
  
  纸质出版：2021-08-30
- 稿件说明：
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任梦雪, 吴广延, 高兴, 等. 不同致痒剂诱导建立大鼠瘙痒模型的效果分析[J]. 第三军医大学学报, 2021,43(16):1527-1534.

Mengxue REN, Guangyan WU, Xing GAO, et al. Comparison of rat itch model induced by different pruritogens[J]. Journal of Third Military Medical University, 2021, 43(16): 1527-1534.
任梦雪, 吴广延, 高兴, 等. 不同致痒剂诱导建立大鼠瘙痒模型的效果分析[J]. 第三军医大学学报, 2021,43(16):1527-1534. DOI： 10.16016/j.1000-5404.202103036.

Mengxue REN, Guangyan WU, Xing GAO, et al. Comparison of rat itch model induced by different pruritogens[J]. Journal of Third Military Medical University, 2021, 43(16): 1527-1534. DOI： 10.16016/j.1000-5404.202103036.

摘要

目的

对比分析不同致痒剂诱导大鼠瘙痒模型的效果。

方法

选择3~4月龄健康雄性SD大鼠240只，分为组胺依赖性致痒剂(磷酸组胺组、化合物48/80组)和非组胺依赖性致痒剂(5-羟色胺组、氯喹组、内皮素-1组)与生理盐水对照组，每组40只。选取颈部和脸颊作为致痒区域，比较皮内注射后30 min内，注射不同剂量(低、中、高浓度)致痒剂后的致痒效果。采用后肢磁环结合行为记录箱系统记录大鼠后肢抓挠次数的差异；运用高浓度致痒剂注射后，采用多道生理信号采集处理系统和甲苯胺蓝染色技术观察并对比分析致痒区域中皮肤短时内感觉神经末梢诱发电位的变化和皮下组织中肥大细胞的变化。

结果

在组胺依赖性致痒剂组中，与生理盐水对照组比较，仅化合物48/80在中、高浓度时可诱发颈部和脸颊的抓挠次数增多(

＜0.05)；皮内高浓度注射时可记录到感觉神经末梢诱发电位频率增多、振幅升高(

＜0.05)，在皮下组织血管旁观察到肥大细胞数目显著增多(

＜0.05)。在非组胺依赖性致痒剂组中，与生理盐水对照组比较，5-羟色胺、氯喹以及内皮素-1(中、高浓度)均能在颈部和脸颊成功诱发抓挠次数增多，差异具有统计学意义(

＜0.05)；当采用皮内高浓度注射时，可记录到感觉神经末梢诱发电位频率增加，振幅升高(

＜0.05)及皮下组织血管旁肥大细胞数目明显增多(

＜0.05)。

结论

5-羟色胺诱导大鼠瘙痒效果最佳，即低浓度能成功建模；而组胺依赖性致痒剂化合物48/80、非组胺依赖性致痒剂氯喹和内皮素-1仅在中、高浓度有效；组胺未能成功诱发出搔抓行为，难以用于建模。

Abstract

Objective

To compare and analyze the effectiveness of different pruritogens on the itch model in rats.

Methods

A total of 240 healthy male SD rats aged 3 to 4 months were selected and divided into 3 groups: histamine-dependent pruritogens group (histamine phosphate

compound 48/80)

non-histamine-dependent pruritogens group (5-HT

chloroquine and endothelin-1) and normal saline control group. The neck and cheek were selected as the itching area to compare the itching effects of different doses (low

medium and high concentrations) of pruritogens within 30 min after intradermal injection. The difference in the frequency of hind limb scratching was recorded by using the hind limb magnetic ring combined with behavior recording box system. The changes of sensory nerve endings evoked potential in the skin and the mast cells in subcutaneous tissue were observed and analyzed by means of multi-channel physiological signal acquisition and processing system and toluidine blue staining.

Results

In the histamine-dependent pruritogens group

only compound 48/80 at medium and high concentrations induced the increment of times of neck and cheek scratching

which was significantly different from the control group (

0.05); increases in frequency and amplitude of sensory nerve endings evoked potential (

0.05); and elevated number of mast cells near the blood vessels in the subcutaneous tissue (

0.05). And

increased times of scratching in neck and cheek were observed in the phosphohistamine group after injection of the 3 concentrations (

0.05). High concentration enhanced the frequency of subcutaneous evoked potential and increased the number of mast cells between the control gro

up and the histamine phosphate group (both

0.05).

Conclusion

The best itch effectiveness is induced by 5-HT in rats

even with low concentration. While

histamine-dependent pruritogens compound 48/80 and non-histamine-dependent pruritogens chloroquine and endothelin-1 are only effective at medium to high concentrations. Histamine fails to induce scratching behaviors

and is difficult to induce the model.

关键词

Keywords

references

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