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Fscb /Cabyr 双基因敲除对雄鼠繁殖能力的影响Effects of
Fscb /Cabyr double gene knockout on reproductive capacity in male mice- 第三军医大学学报 2021年43卷第16期 页码：1521-1526
- 作者机构：
1.400042 重庆，陆军军医大学(第三军医大学)大坪医院泌尿外科
2.400038 重庆，陆军军医大学(第三军医大学)预防医学院毒理学研究所
3.463000 河南 驻马店，联勤保障部队第 990 医院泌尿外科
- 作者简介：
[李彦锋，E-mail: 1738401063@qq.comLI Yanfeng, E-mail: 1738401063@qq.com
- 基金信息：国家自然科学基金面上项目(81571491)
DOI：10.16016/j.1000-5404.202102131
中图分类号： R321.1;R329.2;R698.2收稿：2021-02-24，
修回：2021-05-06，
纸质出版：2021-08-30
- 稿件说明：
移动端阅览
李中泰, 李彦锋, 刘晋祎, 等.
Fscb /Cabyr 双基因敲除对雄鼠繁殖能力的影响[J]. 第三军医大学学报, 2021,43(16):1521-1526.Zhongtai LI, Yanfeng LI, Jinyi LIU, et al. Effects of
Fscb /Cabyr double gene knockout on reproductive capacity in male mice[J]. Journal of Third Military Medical University, 2021, 43(16): 1521-1526.李中泰, 李彦锋, 刘晋祎, 等.
Fscb /Cabyr 双基因敲除对雄鼠繁殖能力的影响[J]. 第三军医大学学报, 2021,43(16):1521-1526. DOI： 10.16016/j.1000-5404.202102131.Zhongtai LI, Yanfeng LI, Jinyi LIU, et al. Effects of
Fscb /Cabyr double gene knockout on reproductive capacity in male mice[J]. Journal of Third Military Medical University, 2021, 43(16): 1521-1526. DOI： 10.16016/j.1000-5404.202102131.
摘要
目的
2
观察
Fscb
/
Cabyr
双基因和单基因敲除雄鼠繁殖能力，探讨CABYR蛋白及与FSCB的复合物对于维持精子授精能力
的价值及其发挥生理功能的可能机制。
方法
2
取
Fscb
/
Cabyr
(--/--)双基因敲除纯合子雄鼠、
Fscb
/
Cabyr
(++/--)单基因敲除雄鼠及野生型雄鼠分别与同代野生型雌鼠交配，观察各组雌鼠怀孕、产仔情况；应用Fluo-4 AM钙离子荧光探针对不同基因型雄鼠精子进行标记，荧光显微镜下观察并应用酶标仪检测各基因型小鼠精子中钙离子水平；Western blot检测各基因型小鼠精子中各种CATSPER通道蛋白的表达情况。
结果
2
所有
Fscb
/
Cabyr
(--/--)双基因敲除与
Cabyr
单基因敲除纯合子雄鼠繁殖能力完全丧失，均未能使任何雌鼠怀孕和产仔。与野生型小鼠相比，
Fscb
/
Cabyr
(--/--)及
Fscb
/
Cabyr
(++/--)基因型雄鼠精子内钙离子浓度显著降低(
P
＜0.000 1)；
Fscb
/
Cabyr
(--/--)型小鼠精子中的CATSPER-1蛋白表达显著减少。
Fscb
/
Cabyr
(--/--)、
Fscb
/
Cabyr
(++/--)型小鼠精子中的CATSPER-4蛋白表达显著减低。
结论
2
雄鼠精子CABYR及FSCB/CABYR蛋白复合物的缺失导致雄鼠繁殖功能的丧失，可能是通过影响精子内的钙通道蛋白CATSPER-1和CATSPER-4的表达和钙离子浓度水平，从而影响精子的运动功能而实现的。
Abstract
Objective
2
To evaluate the value of CABYR protein and its complex with FSCB in maintaining sperm fertilization ability and investigate the possible mechanism of physiological function of the coruplex by observing the natural fertility of
Fscb
/
Cabyr
double gene knockout and
Cabyr
single gene knockout male mice.
Methods
2
Fscb
/
Cabyr
(--/--) double gene knockout male mice
Fscb
/
Cabyr
(++/--) single gene knockout male mice and wild background male mice were mated with wild-type female mice respectively
and the pregnancy and litter production of female mice in each group were observed. The sperm of male mice with different genotypes were labeled with Fluo-4 AM calcium fluorescent probe
in vitro
and observed under fluorescence microscope
and the level of calcium ion in the sperm were detected by ELISA;
The expression of CATSPER channel proteins in sperm of mice with different genotypes was measured by Western blotting.
Results
2
All the male mice with
Fscb
/
Cabyr
(--/--) double gene knockout and
Fscb
/
Cabyr
(++/--) single gene knockout lost their reproductive capacity completely
and failed to make any female mice pregnant and give birth. Compared with the wild-type male mice
the sperm calcium ion concentration of
Fscb
/
Cabyr
(--/--) and
Fscb
/
Cabyr
(++/--) male mice was decreased significantly (
P
<
0.000 1); The expression of CATSPER-1 protein in the sperm of
Fscb
/
Cabyr
(--/--) mice was decreased obviously. The expression of CATSPER-4 in the sperm of
Fscb
/
Cabyr
(--/--) and
Fscb
/
Cabyr
(++/--) mice were notably decreased.
Conclusion
2
The deletion of either CABYR or FSCB/CABYR protein complex in male sperm leads to the loss of reproductive function in the male mice
which may be realized by affecting the expression of calcium channel proteins CATSPER-1 and CATSPER-4
concentration of calcium ions in sperm
and then motility function of sperm.
关键词
Keywords
references
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