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具核梭杆菌介导MUC1在结直肠癌中差异表达的验证
Differential expression of MUC1 gene mediated by
Fusobacterium nucleatum in colorectal cancer cells- 第三军医大学学报 2021年43卷第15期 页码：1501-1507
- 作者机构：
1.401120 重庆，重庆医科大学附属第三医院普外科
2.400038 重庆，陆军军医大学(第三军医大学)药学与检验医学系临床微生物与免疫学教研室
- 作者简介：
[曾冬竹，E-mail：650777@hospital.cqmu.edu.cn ZENG Dongzhu, E-mail: 650777@hospital.cqmu.edu.cn
- 基金信息：重庆市渝北区科技计划项目(2020-35)
DOI：10.16016/j.1000-5404.202101166
中图分类号： R378.2;R394.3;R735.35收稿：2021-01-20，
修回：2021-03-30，
纸质出版：2021-08-15
- 稿件说明：
移动端阅览
叶新力, 李静, 唐彬, 等. 具核梭杆菌介导MUC1在结直肠癌中差异表达的验证[J]. 第三军医大学学报, 2021,43(15):1501-1507.
Xinli YE, Jing LI, Bin TANG, et al. Differential expression of MUC1 gene mediated by
Fusobacterium nucleatum in colorectal cancer cells[J]. Journal of Third Military Medical University, 2021, 43(15): 1501-1507.叶新力, 李静, 唐彬, 等. 具核梭杆菌介导MUC1在结直肠癌中差异表达的验证[J]. 第三军医大学学报, 2021,43(15):1501-1507. DOI： 10.16016/j.1000-5404.202101166.
Xinli YE, Jing LI, Bin TANG, et al. Differential expression of MUC1 gene mediated by
Fusobacterium nucleatum in colorectal cancer cells[J]. Journal of Third Military Medical University, 2021, 43(15): 1501-1507. DOI： 10.16016/j.1000-5404.202101166.
摘要
目的
2
通过生物信息学分析具核梭杆菌(
Fusobacterium nucleatum，F.nucleatum，Fn.
)对结直肠癌(colorectal cancer
CRC)差异表达基因(differential expression genes
DEGs)的调控作用，鉴定关键基因在
F.nucleatum
感染结直肠癌细胞中的表达。
方法
2
利用GEO数据库高通量筛选
F.nucleatum
诱导的结直肠癌差异表达基因；基因本体论(GO)，京都基因和基因组百科全书(KEGG)富集分析差异表达基因数据集；Cytoscape软件构建差异表达基因的蛋白质-蛋白质相互作用网络(PPI)；RT-qPCR和Western blot验证关键(Hub)基因MUC1在细胞模型中的转录水平和蛋白水平表达。
结果
2
共筛选出118个DEGs，其中20个表达上调，98个下调；GO分析显示DEGs在质膜和质膜的组成部分等细胞成分中富集最显著，KEGG分析发现其在PI3K-Akt、MAPK、Rap1和JAK-STAT等信号通路中有明显富集(
P
<
0.05)。在PPI网络中，Hub基因MUC1具有较高的中心性得分，
F.nucleatum
感染与MUC1的差异表达显著相关。
结论
2
通过对关键基因的筛选和验证，
F.nucleatum
感染致CRC的机制可能与MUC1的过表达有关。
Abstract
Objective
2
To investigate the regulatory effect of
Fusobacterium nucleatum
(
F.nucleatum
) on differential expression genes (DEGs) in colorectal cancer (CRC) by bioinformatics analysis
and to identify the expression of key genes in
F.nucleatum
infected CRC cells.
Methods
2
The high-throughput sequencing data of DEGs in CRC regulated by
F.nucleatum
were downloaded and screened from Gene Expression Omnibus (GEO) database
then Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed on the datasets. Protein-protein interaction (PPI) network of DEGs were subsequently constructed with Cytoscape software. Finally
Western blotting and RT-qPCR were employed to verity the expression of hub gene MUC1 at protein and mRNA levels in the cell model
respectively.
Results
2
A total of 118 DEGs were screened out
of which 20 were up-regulated and 98 down-regulated. GO analysis showed that DEGs were noticeably enriched in cellular component (CC) such as plasma membrane and integral component of membrane
and KEGG analysis found that DEGs were significantly enriched in PI3K-Akt
MAPK
Rap1 and JAK-STAT signaling pathways (
P
<
0.05). In the PPI network
the hub gene MUC1 presented a high centrality score. Western blotting and RT-qPCR indicated that the infection of
F.nucleatum
was significantly correlated with the differential expression of MUC1.
Conclusion
2
The mechanism of CRC induced by
F.nucleatum
infection may be related to the overexpression of MUC1
according to the screening and verification of hub genes.
关键词
Keywords
references
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