LncRNA SNHG12通过miR-129-5p/GTPBP4对肝细胞癌进展的促进作用

章诺贝; 沈浩; 黄神安; 陈新

doi:10.16016/j.1000-5404.202101151

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LncRNA SNHG12通过miR-129-5p/GTPBP4对肝细胞癌进展的促进作用

基础医学 | 更新时间：2026-01-09

- LncRNA SNHG12通过miR-129-5p/GTPBP4对肝细胞癌进展的促进作用
- Long non-coding RNA SNHG12 promotes progression of hepatocellular carcinoma through miR-129-5p/GTPBP4
- 第三军医大学学报 2021年43卷第18期页码：1783-1795
- 作者机构：
  
  1.330000 南昌, 南昌大学第二附属医院：消化内科
  2.330000 南昌, 南昌大学第二附属医院：核医学科
- 作者简介：
  
  [陈新, E-mail：13870979404@163.com CHEN Xin, 13870979404@163.com
- 基金信息：
  
  国家自然基金地区科学基金项目(81760427);江西省自然科学基金(20202BABL206094);江西省自然科学基金(20202BABL216037);江西省卫生健康委科技计划(20201037);江西省卫生健康委科技计划(202110042)
- DOI：10.16016/j.1000-5404.202101151
  中图分类号： R394.3;R730.23;R735.7
- 收稿：2021-01-18，
  
  修回：2021-05-06，
  
  纸质出版：2021-09-30
- 稿件说明：
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章诺贝, 沈浩, 黄神安, 等. LncRNA SNHG12通过miR-129-5p/GTPBP4对肝细胞癌进展的促进作用[J]. 第三军医大学学报, 2021,43(18):1783-1795.

Nuobei ZHANG, Hao SHEN, Shen'an HUANG, et al. Long non-coding RNA SNHG12 promotes progression of hepatocellular carcinoma through miR-129-5p/GTPBP4[J]. Journal of Third Military Medical University, 2021, 43(18): 1783-1795.
章诺贝, 沈浩, 黄神安, 等. LncRNA SNHG12通过miR-129-5p/GTPBP4对肝细胞癌进展的促进作用[J]. 第三军医大学学报, 2021,43(18):1783-1795. DOI： 10.16016/j.1000-5404.202101151.

Nuobei ZHANG, Hao SHEN, Shen'an HUANG, et al. Long non-coding RNA SNHG12 promotes progression of hepatocellular carcinoma through miR-129-5p/GTPBP4[J]. Journal of Third Military Medical University, 2021, 43(18): 1783-1795. DOI： 10.16016/j.1000-5404.202101151.

摘要

目的

探讨长链非编码RNA(LncRNA)核仁小RNA宿主基因12(small nucleolar RNA host gene 12

SNHG12)对肝细胞癌(hepatocellular carcinoma，HCC)发展的调控机制。

方法

通过RT-qPCR检测长链非编码RNA(long noncoding RNA，LncRNA) SNHG12在肝癌组织和肝癌细胞系中的表达水平；分析肝癌组织中LncRNA SNHG12的表达水平与HCC患者临床病理参数的关系；运用MTT实验、细胞集落形成实验、Transwell实验、划痕愈合实验、裸鼠成瘤实验检测LncRNA SNHG12、miR-129-5p、GTPBP4在肝癌细胞活性、增殖、迁移、侵袭中的作用；采用荧光素酶报告基因、RT-qPCR、Western blot实验来评估LncRNA SNHG12、miR-129-5p、GTPBP4之间的互相作用。

结果

LncRNA SNHG12在肝癌组织和肝癌细胞系中表达上调(

0.05)；LncRNA SNHG12表达水平较高的HCC患者相比其表达水平较低的患者预后更差(

0.05)；LncRNA SNHG12表达水平与肿瘤大小、TNM分期、淋巴结转移存在正相关的关系；LncRNA SNHG12可促进HCC的细胞活性、增殖、迁移及侵袭(

0.05)；miR-129-5p在肝癌组织中表达下调，其可抑制肝癌细胞活性、增殖、迁移及侵袭(

0.05)；LncRNA SNHG12可通过与miR-129-5p进行竞争性结合，从而促进GTPBP4的表达(

0.05)；GTPBP4在HCC组织中表达上调，其可促进肝癌细胞活性、增殖、迁移及侵袭(

0.05)。

结论

LncRNA SNHG12可通过调控miR-129-5p/GTPBP4轴从而促进HCC的进展。

Abstract

Objective

To explore the regulatory mechanism of long non-coding RNA (LncRNA) small nucleolar RNA host gene 12 (SNHG12) on the development of hepatocellular carcinoma (HCC).

Methods

The expression levels of SNHG12 in HCC tissues as well as in HCC cell lines were detected by RT-qPCR

and the correlation between the SNHG12 level and the clinicopathological parameters of HCC patients was then analyzed. The effects of SNHG12

miR-129-5p and GTPBP4 on the activity

proliferation

migration and invasion of HCC cells were determined using MTT assay

cell colony formation test

Transwell test

scratch healing assay and xenograft tumor growth assay

respectively. Finally

luciferase reporter gene

RT-qPCR and Western blotting were employed to evaluate the interactions among SNHG12

miR-129-5p and GTPBP4.

Results

The expression of SNHG12 was up-regulated in both HCC tissues and HCC cell lines (

0.05). HCC patients with higher expression level of SNHG12 had a worse prognosis than those with lower level (

0.05)

and its level was positively correlated with tumor size

TNM stage and lymph node metastasis. LncRNA SNHG12 promoted the viability

proliferation

migration and invasion of HCC cells (

0.05)

whereas

miR-129-5p was down-regulated in HCC tissues and showed inhibitory effects on above biological behaviors of HCC cells (

0.05). Moreover

through competitively binding with miR-129-5p

SNHG12 promoted GTPBP4 expression (

0.05)

which was up-regulated in HCC tissues and facilitated the activity

proliferation

migration and invasion of HCC cells (

0.05).

Conclusion

LncRNA SNHG12 promotes the progression of HCC by regulating the miR-129-5p/GTPBP4 axis.

关键词

Keywords

references

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