使用7.0T MRI信号强度比和扩散张量成像定量分析小鼠脱髓鞘模型的研究

丁爽; 杜思霖; 祝琦源; 严子淳; 李咏梅; 曾春

doi:10.16016/j.1000-5404.202101113

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使用7.0T MRI信号强度比和扩散张量成像定量分析小鼠脱髓鞘模型的研究

基础医学 | 更新时间：2026-01-09

- 使用7.0T MRI信号强度比和扩散张量成像定量分析小鼠脱髓鞘模型的研究
- Quantitative analysis of demyelination mouse model using signal intensity ratio and diffusion tensor imaging at 7.0 Tesla MRI
- 第三军医大学学报 2021年43卷第13期页码：1227-1234
- 作者机构：
  
  400016 重庆，重庆医科大学附属第一医院放射科
- 作者简介：
  
  [曾春，E-mail: zengchun19840305@163.comZENG Chun, E-mail: zengchun19840305@163.com
- 基金信息：
  
  重庆市卫健委医学科研青年项目(2018QNXM004)
- DOI：10.16016/j.1000-5404.202101113
  中图分类号： R-332;R445.2;R744.51
- 收稿：2021-01-13，
  
  修回：2021-03-23，
  
  纸质出版：2021-07-15
- 稿件说明：
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丁爽, 杜思霖, 祝琦源, 等. 使用7.0T MRI信号强度比和扩散张量成像定量分析小鼠脱髓鞘模型的研究[J]. 第三军医大学学报, 2021,43(13):1227-1234.

Shuang DING, Silin DU, Qiyuan ZHU, et al. Quantitative analysis of demyelination mouse model using signal intensity ratio and diffusion tensor imaging at 7.0 Tesla MRI[J]. Journal of Third Military Medical University, 2021, 43(13): 1227-1234.
丁爽, 杜思霖, 祝琦源, 等. 使用7.0T MRI信号强度比和扩散张量成像定量分析小鼠脱髓鞘模型的研究[J]. 第三军医大学学报, 2021,43(13):1227-1234. DOI： 10.16016/j.1000-5404.202101113.

Shuang DING, Silin DU, Qiyuan ZHU, et al. Quantitative analysis of demyelination mouse model using signal intensity ratio and diffusion tensor imaging at 7.0 Tesla MRI[J]. Journal of Third Military Medical University, 2021, 43(13): 1227-1234. DOI： 10.16016/j.1000-5404.202101113.

摘要

目的

用7.0 T MRI扫描改良双环己酮草酰二腙(cuprizone

CPZ)小鼠多发性硬化(multiple sclerosis

MS)脱髓鞘模型，探讨信号强度比(signal intensity ratio

SIR)联合扩散张量成像(diffusion tensor imaging

DTI)定量检测和分析小鼠MS模型灰、白质脱髓鞘的价值。

方法

20只小鼠使用随机数字表法分为实验组(

=10)及对照组(

=10)，分别使用CPZ-羧甲基纤维素钠(sodium carboxymethyl cellulose

CMCNa)悬浊液及0.5% CMCNa溶液灌胃6周后，在7.0T MRI上对两组小鼠行T

WI及T

WI扫描，在空间配准后计算SIR，同时行DTI扫描获取各向异性分数(fractional anisotropy，FA)、平均扩散率(mean diffusivity，MD)等扩散张量参数，并行脑组织病理染色。选择胼胝体(corpus callosum，CC)、双侧大脑皮层(cortex，CTX)、双侧丘脑(thalamus，THA)及双侧海马体(hippocampus，HIP)为感兴趣区(regions of interest，ROI)，比较两组SIR、FA、MD及病理染色平均灰度值(mean grayscale value，M

GSV)的差异，并行影像及病理参数的两两相关分析及线性拟合。

结果

与对照组相比，实验组小鼠CC呈明显脱髓鞘改变，SIR及FA值显著降低(

0.05)，而MD值差异无统计学意义，病理MGSV显著升高(

0.05)；其余部位两组SIR、FA、MD值及病理MGSV比较差异均无统计学意义。相关性分析显示SIR、FA值均与MGSV呈负相关，且|

SIR

|。

结论

利用SIR联合DTI可以定量评估MS模型白质脱髓鞘改变。

Abstract

Objective

To assess the value of combined signal intensity ratio (SIR) and diffusion tensor imaging (DTI) measured at 7.0 T MRI in quantitative detection and analysis of gray and white matter demyelination in the modified cuprizone (CPZ)-induced mouse model of multiple sclerosis (MS).

Methods

A total of 20 mice were randomly divided into experimental group (

=10) and control group (

=10)

and were intragastrically administrated with CPZ-carboxymethyl cellulose sodium (CMCNa) suspension and 0.5% CMCNa solution

respectively. In 6 weeks after administration

the mice of both groups were given T

WI and T

WI scanning on 7.0 T MRI

and the SIR value was calculated by linear registration of T

WI and T

WI. Besides

DTI scanning was also conducted in both groups to obtain the diffusion parameters such as fractional anisotropy (FA) and mean diffusivity (MD). Moreover

pathological observation was performed on cerebral tissues after Luxol fast blue staining for mean grayscale value (MGSV). Then corpus callosum (CC)

bilateral cerebral cortex (CTX)

bilateral thalamus (THA)

and bilateral hippocampus (HIP) were selected as the regions of interest (ROI). SIR

MD and MGSV of above regions were subsequently compared between the experimental and control groups. Finally

pairwise correlation analysis and linear fitting were carried out on the MRI metrics and pathological parameters.

Results

As compare

d with the control group

the CC region in the experimental group showed significant demyelinating changes

with lower SIR and FA values and higher MGSV (

0.05)

while the MD value showed no statistical significance (

0.05). However

in terms of other cerebral regions

there were no significant differences between the 2 groups in SIR

MD and MGSV values (

0.05). Correlation analysis suggested that both SIR and FA values were negatively correlated with MGSV

with

SIR

Conclusion

SIR combined with DTI can quantitatively evaluate the demyelination changes of white matter in MS model.

关键词

Keywords

references

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