人体肝功能单元结构三维构筑的初步探索

杨静怡; 刘红军; 陈娜; 侯宇丰; 吕沄禧; 李其; 梅峰; 平轶芳; 郑璐; 吴毅

doi:10.16016/j.1000-5404.202101095

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人体肝功能单元结构三维构筑的初步探索

专题报道：数字医学与人工智能 | 更新时间：2026-01-09

- 人体肝功能单元结构三维构筑的初步探索
- Preliminary exploration of architecture of human hepatic functional unit
- 第三军医大学学报 2021年43卷第18期页码：1713-1719
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)：生物医学工程与影像医学系数字医学教研室
  2.400038 重庆，陆军军医大学(第三军医大学)：基础医学院学员五大队十五中队
  3.400038 重庆，陆军军医大学(第三军医大学)：生物医学工程与影像医学五大队十七队
  4.400038 重庆，陆军军医大学(第三军医大学)：基础医学院组织胚胎学教研室
  5.400038 重庆，陆军军医大学(第三军医大学)：附属第一医院病理科
  6.400037 重庆，陆军军医大学(第三军医大学)附属第二医院肝胆科
- 作者简介：
  
  [吴毅，E-mail：wuy1979@tmmu.edu.cn WU Yi, E-mail: wuy1979@tmmu.edu.cn
- 基金信息：
  
  国家自然科学基金面上项目(31671251);重庆英才青年拔尖人才项目(CQYC201905037);陆军科技英才(91003201901973)
- DOI：10.16016/j.1000-5404.202101095
  中图分类号： R329.2;R329-3;R329.4
- 收稿：2021-01-11，
  
  修回：2021-04-30，
  
  纸质出版：2021-09-30
- 稿件说明：
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杨静怡, 刘红军, 陈娜, 等. 人体肝功能单元结构三维构筑的初步探索[J]. 第三军医大学学报, 2021,43(18):1713-1719.

Jingyi YANG, Hongjun LIU, Na CHEN, et al. Preliminary exploration of architecture of human hepatic functional unit[J]. Journal of Third Military Medical University, 2021, 43(18): 1713-1719.
杨静怡, 刘红军, 陈娜, 等. 人体肝功能单元结构三维构筑的初步探索[J]. 第三军医大学学报, 2021,43(18):1713-1719. DOI： 10.16016/j.1000-5404.202101095.

Jingyi YANG, Hongjun LIU, Na CHEN, et al. Preliminary exploration of architecture of human hepatic functional unit[J]. Journal of Third Military Medical University, 2021, 43(18): 1713-1719. DOI： 10.16016/j.1000-5404.202101095.

摘要

目的

通过数字化技术三维构筑人体肝功能单元，并对其三维形态和空间毗邻关系进行研究，以了解肝功能单元肝细胞的三维构筑规律。

方法

选择人体肝组织，通过常规石蜡连续切片、HE染色和显微镜扫描成像，获取4 μm厚度的肝组织学切片，使用Photoshop软件对切片染色图像进行配准，使用AMIRA软件对肝功能单元包括肝小叶轮廓、肝血窦、肝细胞索、中央静脉、小叶间动脉、静脉和胆管进行三维重建，对其进行组织形态学参数测量和研究。

结果

构筑了肝功能单元的三维数字化模型，结果显示肝小叶外轮廓为不规则的较圆钝的多边形结构，边长不等

最大直径可达1 495.27 μm，最小直径为1 036.96 μm；肝小叶的中央静脉并不位于肝小叶正中，依次表现为322.38、474.05、878.7、880.16 μm；肝血窦为相互交织的血管网，并不构成放射状排列的血管网。

结论

本研究中的三维重建图能够精准反映肝功能单元的三维形态和空间毗邻关系，为建立肝脏微血管系统提供研究基础。

Abstract

Objective

To construct a three-dimensional of human hepatic functional unit through digital technology

and study its 3D shape and spatial adjoining relationship in order to understand the construction law of hepatic functional unit hepatic lobule cells.

Methods

We selected human hepatic lobule tissue

and used conventional paraffin serial sections

HE staining technology and microscope scanning to obtain hepatic lobule section in 4-μm thickness. We employed Photoshop software for image registration and used AMIRA software 2019.1 version for 3D reconstruction of hepatic functional unit which include hepatic lobule. Lobular contours

hepatic sinusoids

hepatocyte cords

central veins

interlobular arteries

veins and bile ducts were measured and studied for the tissue morphological parameters.

Results

A 3D digital model of the hepatic functional unit was reconstructed

and we founded that the contour of the hepatic lobule was irregularly round and blunt polygon structure with different edge lengths. The maximum diameter was 1 495.27 μm and the minimum diameter was 1 036.96 μm; The central vein of the hepatic lobule was not located in the middle of the hepatic lobule

at 322.38

474.05

878.7 and 880.16 μm

The hepatic sinusoids were interwoven vascular networks

which did not constitute a radial arrangement of blood vessel networks.

Conclusion

The Results of our 3D morphological study of hepatic lobule are different from the traditional anatomy textbooks and atlas

which not only update our knowledge of the structure of hepatic functional unit

and provide a 3D visualization image of the real human hepatic lobule structure. However

we think that the 3D morphology of hepatic functional unit structure is produced under the condition of hepatic lobule cell shaping development and reshaping redevelopment

therefore it provides a computer model for the 3D printing of hepatic lobule tissue cells.

关键词

Keywords

references

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