miR-3934-5p靶向抑制β内酰胺酶表达促进肝癌细胞增殖

鲜瑶; 刘润坤; 莫缓椰; 肖雪莲; 王亮; 张雷

doi:10.16016/j.1000-5404.202012245

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miR-3934-5p靶向抑制β内酰胺酶表达促进肝癌细胞增殖

基础医学 | 更新时间：2026-01-09

- miR-3934-5p靶向抑制β内酰胺酶表达促进肝癌细胞增殖
- MiR-3934-5p promotes proliferation of hepatocellular carcinoma cells by targeting LACTB
- 第三军医大学学报 2021年43卷第13期页码：1219-1226
- 作者机构：
  
  1.710061 西安，西安交通大学第一附属医院: 营养科
  2.710061 西安，西安交通大学第一附属医院: 肝胆外科
  3.710061 西安，西安交通大学第一附属医院: 老年外科
- 作者简介：
  
  [张雷，E-mail: zhanglei0519@xjtu.edu.cnZHANG Lei, E-mail: zhanglei0519@xjtu.edu.cn
- 基金信息：
  
  陕西省重点研发计划(2020SF-061);西安交通大学第一附属医院院基金(2019ZYTS-07)
- DOI：10.16016/j.1000-5404.202012245
  中图分类号： R394.3;R730.23;R735.7
- 收稿：2020-12-31，
  
  修回：2021-02-08，
  
  纸质出版：2021-07-15
- 稿件说明：
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鲜瑶, 刘润坤, 莫缓椰, 等. miR-3934-5p靶向抑制β内酰胺酶表达促进肝癌细胞增殖[J]. 第三军医大学学报, 2021,43(13):1219-1226.

Yao XIAN, Runkun LIU, Huanye MO, et al. MiR-3934-5p promotes proliferation of hepatocellular carcinoma cells by targeting LACTB[J]. Journal of Third Military Medical University, 2021, 43(13): 1219-1226.
鲜瑶, 刘润坤, 莫缓椰, 等. miR-3934-5p靶向抑制β内酰胺酶表达促进肝癌细胞增殖[J]. 第三军医大学学报, 2021,43(13):1219-1226. DOI： 10.16016/j.1000-5404.202012245.

Yao XIAN, Runkun LIU, Huanye MO, et al. MiR-3934-5p promotes proliferation of hepatocellular carcinoma cells by targeting LACTB[J]. Journal of Third Military Medical University, 2021, 43(13): 1219-1226. DOI： 10.16016/j.1000-5404.202012245.

摘要

目的

观察miR-3934-5p在肝细胞癌(hepatocellular carcinoma

HCC)中的表达，探讨其临床意义及分子机制。

方法

收集53例2013年9月至2014年8月在本院确诊的肝细胞癌患者的癌旁组织及肿瘤组织。实时定量PCR(RT-qPCR)检测组织和细胞miR-3934-5p的表达；应用人类癌症基因库(the Cancer Genome Atlas

TCGA)分析正常肝组织与肝癌组织中miR-3934-5p的表达；用χ

检验和Kaplan-Meier生存曲线分析miR-3934-5p与临床病理特征及生存预后之间的相关性；采用RT-qPCR和Western blot检测转染效率；CCK-8实验、平板克隆实验及EdU实验评价HCCLM3细胞的活性及增殖能力；分析HCC组织中β-内酰胺酶(beta-lactamases，LACTB) mRNA与miR-3934-5p表达的相关性；TargetScan数据库预测miR-3934-5p下游靶基因，通过荧光素酶报告基因实验加以验证。

结果

miR-3934-5p在肝癌组织及细胞中高表达(

0.05)，且其高表达与肿瘤大小、肿瘤数量、血管侵犯及TNM分期显著相关(

0.05)；与miR-3934-5p低表达组相比，miR-3934-5p高表达组的肝癌患者5年生存率更低；CCK-8实验、平板克隆实验及EdU实验结果证实: 敲减miR-3934-5p可显著抑制HCCLM3细胞的增殖能力(

0.05)；LACTB被证实是miR-3934-5p的直接作用靶点，且其介导了miR-3934-5p的生物学功能。

结论

miR-3934-5p通过靶向抑制抑癌基因LACTB的表达促进肝癌细胞的增殖能力。

Abstract

Objective

To explore the expression

clinical significance and molecular mechanism of miR-3934-5p in hepatocellular carcinoma (HCC).

Methods

HCC tissues and adjacent normal tissues were collected from 53 HCC patients diagnosed in our hospital from September 2013 to August 2014. Real-time quantitative PCR (RT-qPCR) was performed to detect the level of miR-3934-5p in the above tissues and human liver cell line L02 and HCC cell lines. The Cancer Genome Atlas (TCGA) database was used to analyze the expression of miR-3934-5p in normal liver tissues and HCC tissues;

Chi

-square test with Kaplan-Meier survival curve were applied to analyze the correlation of miR-3934-5p and clinicopathological characteristics and survival prognosis; RT-qPCR and Western blotting were adopted to detect transfection efficiency; CCK-8 assay

colony formation assay and EdU assay were employed respectively to evaluate the activity and proliferation of HCCLM3 cells; Spearman correlation analysis was applied to study the correlation between LACTB mRNA and miR-3934-5p expression i

n HCC tissues; TargetScan database was used to predict the downstream target genes of miR-3934-5p

and the prediction results was verified by luciferase reporter assay.

Results

MiR-3934-5p was highly expressed in the liver cancer tissues and cells (

0.05)

and its high expression was significantly correlated with tumor size (

=0.040)

tumor multiplicity (

=0.013)

vascular invasion (

=0.006)

and TNM stage (

=0.017); Compared to the low miR-3934-5p expression group

the HCC patients with high expression had poorer survival prognosis. CCK-8 assay

colony formation assay and EdU assay confirmed that knocking down miR-3934-5p notably inhibited the proliferation of HCCLM3 cells (

0.05). LACTB had been proved to be the direct target of miR-3934-5p

and mediated the biological effect of miR-3934-5p on HCC cells.

Conclusion

MiR-3934-5p promotes the proliferation of HCC cells by targeting tumor suppressor gene LACTB.

关键词

Keywords

references

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