CD8<sup>+</sup>CD103<sup>+</sup> Trm细胞在胃癌中的分布、表型及其功能下降的机制研究

沈洋; 彭六生; 邱远; 邹全明; 马代远

doi:10.16016/j.1000-5404.202012107

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CD8⁺CD103⁺ Trm细胞在胃癌中的分布、表型及其功能下降的机制研究

基础医学 | 更新时间：2026-02-13

- CD8⁺CD103⁺ Trm细胞在胃癌中的分布、表型及其功能下降的机制研究
- Distribution, phenotype and dysfunctional mechanism of CD8⁺ CD103⁺ tissue-resident memory T cells in gastric cancer patients
- 第三军医大学学报 2021年43卷第12期页码：1146-1152
- 作者机构：
  
  1.637000 四川南充，川北医学院附属医院肿瘤科
  2.400038 重庆，陆军军医大学(第三军医大学)药学与检验医学系微生物与生化药学教研室
  3.400037 重庆，陆军军医大学(第三军医大学)第二附属医院普外科
- 作者简介：
  
  [邹全明，E-mail: qmzou@tmmu.edu.cn ZOU Quanming, E-mail: qmzou@tmmu.edu.cn
  [马代远，E-mail: mdylx@163.com MA Daiyuan, E-mail: mdylx@163.com
- 基金信息：
  
  国家自然科学基金青年科学基金项目(81502457)
- DOI：10.16016/j.1000-5404.202012107
  中图分类号： R361;R735.2;R730.3
- 收稿：2020-12-13，
  
  修回：2021-02-09，
  
  纸质出版：2021-06-30
- 稿件说明：
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沈洋, 彭六生, 邱远, 等. CD8⁺CD103⁺ Trm细胞在胃癌中的分布、表型及其功能下降的机制研究[J]. 第三军医大学学报, 2021,43(12):1146-1152.

Yang SHEN, Liusheng PENG, Yuan QIU, et al. Distribution, phenotype and dysfunctional mechanism of CD8⁺ CD103⁺ tissue-resident memory T cells in gastric cancer patients[J]. Journal of Third Military Medical University, 2021, 43(12): 1146-1152.
沈洋, 彭六生, 邱远, 等. CD8⁺CD103⁺ Trm细胞在胃癌中的分布、表型及其功能下降的机制研究[J]. 第三军医大学学报, 2021,43(12):1146-1152. DOI： 10.16016/j.1000-5404.202012107.

Yang SHEN, Liusheng PENG, Yuan QIU, et al. Distribution, phenotype and dysfunctional mechanism of CD8⁺ CD103⁺ tissue-resident memory T cells in gastric cancer patients[J]. Journal of Third Military Medical University, 2021, 43(12): 1146-1152. DOI： 10.16016/j.1000-5404.202012107.

摘要

目的

探讨CD8

CD103

常驻记忆型T(Trm)细胞在胃癌患者组织中的分布情况，并研究其表型特征，分

析胃癌组织中CD8

CD103

Trm细胞功能下降的机制。

方法

采用免疫组织化学染色检测胃癌患者组织中CD8

CD103

Trm细胞的数目并分析其与胃癌患者临床分期的相关性；流式细胞术检测胃癌患者组织中CD8

CD103

Trm细胞的比例与数目、表型和功能；体外TGF-β1刺激培养CD8

T细胞，分析其对CD8

CD103

Trm细胞功能的影响。

结果

与正常组织相比，胃癌患者肿瘤组织中CD8

CD103

Trm细胞的数目并未明显改变(

0.05)，但其频率显著下降(

0.05)；胃癌组织浸润的CD8

CD103

Trm细胞主要属于效应记忆型细胞(CD45RA

CCR7

)，并高表达CD69和PD-1；然而，其表达的颗粒酶B和穿孔素水平显著下降(

0.05)；体外TGF-β1可诱导CD8

CD103

Trm细胞下调表达颗粒酶B和穿孔素(

0.05)。此外，与早期胃癌患者相比，晚期胃癌患者肿瘤组织中浸润的CD8

CD103

Trm细胞数目显著下降(

0.05)。

结论

胃癌组织中CD8

CD103

Trm细胞的浸润水平与疾病的进展成负相关，并呈现出抑制性的表型且抗肿瘤功能下降，从而有助于胃癌的免疫逃逸。

Abstract

Objective

To investigate the distribution and phenotypic features of CD8

CD103

tissue-resident memory T cells (Trm) in gastric cancer (GC) patients

and to explore their dysfunctional mechanism.

Methods

The number of CD8

CD103

Trm cells in GC tissues was detected by immunohistochemical staining and its correlation with clinical stages of GC patients was subsequently analyzed. The proportion

phenotype and function of CD8

CD103

Trm cells in tissues of GC patients were determined using flow cytometry. In addition

CD8

T cells were cultured with transforming growth factor-β1(TGF-β1) stimulation

in vitro

and the effects of TGF-β1 on the function of CD8

CD103

Trm cells were explored.

Results

The number of CD8

CD103

Trm cells in GC tissues was not greatly altered (

0.05)

but its proportion in CD8

T cells was significantly declined than that of normal tissues (

0.05). The main population of tumor-infiltrating CD8

CD103

Trm cells belonged to the type of effector memory cells (CD45RA

CCR7

)

which showed higher expression of CD69 and PD-1 while lower expression of granzyme B and perforin than CD8

CD103

T cells (

0.05).

In vitro

culture displayed that TGF-β1 induced the down-regulation of granzyme B and perforin expression in CD8

CD103

Trm cells. Moreover

the number of tumor-infiltrating CD8

CD103

Trm cells in advanced GC patients was found significantly decreased than that in early GC patients (

0.05).

Conclusion

The infiltration level of CD8

CD103

Trm cells in GC tissues is negatively correlated with GC progression. These cells show an inhibitory phenotype as well as decreased anti-tumor function

thus contributing to immune escape of GC.

关键词

Keywords

references

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CD8+CD103+ Trm细胞在胃癌中的分布、表型及其功能下降的机制研究

Distribution, phenotype and dysfunctional mechanism of CD8+ CD103+ tissue-resident memory T cells in gastric cancer patients

DOI：10.16016/j.1000-5404.202012107

摘要

Abstract

关键词

Keywords

references

CD8⁺CD103⁺ Trm细胞在胃癌中的分布、表型及其功能下降的机制研究

Distribution, phenotype and dysfunctional mechanism of CD8⁺ CD103⁺ tissue-resident memory T cells in gastric cancer patients