右美托咪定对七氟醚麻醉诱导新生大鼠海马内质网应激的影响

白鹏祖; 张利亮; 张海盛; 姚泽宇

doi:10.16016/j.1000-5404.202011228

您当前的位置：

首页 >

文章列表页 >

右美托咪定对七氟醚麻醉诱导新生大鼠海马内质网应激的影响

基础医学 | 更新时间：2026-01-09

- 右美托咪定对七氟醚麻醉诱导新生大鼠海马内质网应激的影响
- Effect of dexmedetomidine on sevoflurane-induced endoplasmic reticulum stress in hippocampus of neonatal rats
- 第三军医大学学报 2021年43卷第10期页码：964-969
- 作者机构：
- 作者简介：
- 基金信息：
  
  青海省自然科学基金项目(2019-ZJ-7085)
- DOI：10.16016/j.1000-5404.202011228
  中图分类号：
- 收稿：2020-11-24，
  
  纸质出版：2021-05-30
- 稿件说明：
移动端阅览
白鹏祖, 张利亮, 张海盛, 等. 右美托咪定对七氟醚麻醉诱导新生大鼠海马内质网应激的影响[J]. 第三军医大学学报, 2021,43(10):964-969.

Pengzu BAI, Liliang ZHANG, Haisheng ZHANG, et al. Effect of dexmedetomidine on sevoflurane-induced endoplasmic reticulum stress in hippocampus of neonatal rats[J]. Journal of Third Military Medical University, 2021, 43(10): 964-969.
白鹏祖, 张利亮, 张海盛, 等. 右美托咪定对七氟醚麻醉诱导新生大鼠海马内质网应激的影响[J]. 第三军医大学学报, 2021,43(10):964-969. DOI： 10.16016/j.1000-5404.202011228.

Pengzu BAI, Liliang ZHANG, Haisheng ZHANG, et al. Effect of dexmedetomidine on sevoflurane-induced endoplasmic reticulum stress in hippocampus of neonatal rats[J]. Journal of Third Military Medical University, 2021, 43(10): 964-969. DOI： 10.16016/j.1000-5404.202011228.

摘要

Abstract

Objective

To investigate the mechanism of dexmedetomidine (DEX) in sevoflurane (SEVO) induced hippocampal neuron injury in neonatal rats.

Methods

Forty male Sprague-Dawley (SD) rats (7-day-old

10~15 g) were given i.p. injections of saline or DEX (μg·kg

-1

)

and then exposed for 6 h to 2.5% SEVO. After rat hippocampal tissue was collected and separated

flow cytometry

immunofluorescence staining and Western blotting were used respectively to detect cell apoptosis

expression of endoplasmic reticulum(ER) stress markers PERK and Caspase-12

and expression of MAPK signal pathway-related proteins (ERK

JNK and P38).

Results

The apoptotic rate of hippocampal cells was significantly lower (24.27±4.52

43.90±10.94

0.05)

and the expression of PERK and Caspase-12 was also obviously decreased in the rats after co-administration of SEVO and DEX than those exposed to SEVO alone (

0.05). The expression levels of p-ERK

p-JNK and p-P38 were significantly increased in the rats after SEVO exposure than the control rats (

0.05)

and the levels were lowest in the rats after co-administration (

0.05).

Conclusion

DEX alleviates ER stress probably by inhibiting the MAPK signaling pathway

and then improves neurotoxicity induced by SEVO.

关键词

Keywords

references

杨秋悦贾俊可黄怡自噬在七氟醚麻醉致老龄小鼠认知功能减退中的作用: 与神经发生的关系中华麻醉学杂志 2019 39 7 809 812 10.3760/cma.j.issn.0254-1416.2019.07.011 杨秋悦, 贾俊可, 黄怡, 等. 自噬在七氟醚麻醉致老龄小鼠认知功能减退中的作用: 与神经发生的关系[J]. 中华麻醉学杂志, 2019, 39(7): 809-812. DOI: 10.3760/cma.j.issn.0254- 1416.2019.07.011.

YANG Q Y JIA J K HUANG Y Role of autophagy in cognitive decline caused bysevoflurane anesthesia in aged mice: the relationship with neurogenesis Chin J Anesthesiol 2019 39 7 809 812 10.3760/cma.j.issn.0254-1416.2019.07.011 YANG Q Y, JIA J K, HUANG Y, et al . Role of autophagy in cognitive decline caused bysevoflurane anesthesia in aged mice: the relationship with neurogenesis[J ] . Chin J Anesthesiol, 2019, 39(7): 809-812. DOI: 10.3760/cma.j.issn.0254-1416.2019.07.011.

YUAN Y YANG J L CHEN J Alpha-lipoic acid protects against cadmium-induced neuronal injury by inhibiting the endoplasmic reticulum stress eIF2α-ATF4 pathway in rat cortical neurons in vitro and in vivo Toxicology 2019 414 1 13 10.1016/j.tox.2018.12.005 YUAN Y, YANG J L, CHEN J, et al . Alpha-lipoic acid protects against cadmium-induced neuronal injury by inhibiting the endoplasmic reticulum stress eIF2α-ATF4 pathway in rat cortical neurons in vitro and in vivo [J ] . Toxicology, 2019, 414: 1-13. DOI: 10.1016/j.tox.2018.12.005.

LIU B XIA J M CHEN Y L Sevoflurane-induced endoplasmic reticulum stress contributes to neuroapoptosis and BACE-1 expression in the developing brain: the role of eIF2α Neurotox Res 2017 31 2 218 229 10.1007/s12640-016-9671-z LIU B, XIA J M, CHEN Y L, et al . Sevoflurane-induced endoplasmic reticulum stress contributes to neuroapoptosis and BACE-1 expression in the developing brain: the role of eIF2α[J ] . Neurotox Res, 2017, 31(2): 218-229. DOI: 10.1007/s12640-016-9671-z.

SHEN F Y SONG Y C GUO F Cognitive impairment and endoplasmic reticulum stress induced by repeated short-term sevoflurane exposure in early life of rats Front Psychiatry 2018 9 332 10.3389/fpsyt.2018.00332 SHEN F Y, SONG Y C, GUO F, et al . Cognitive impairment and endoplasmic reticulum stress induced by repeated short-term sevoflurane exposure in early life of rats[J ] . Front Psychiatry, 2018, 9: 332. DOI: 10.3389/fpsyt.2018.00332.

陈佳林周丽芳韦祎右美托咪定对丙泊酚诱导新生大鼠大脑发育的远期影响临床麻醉学杂志 2018 34 2 163 166 10.12089/jca.2018.02.015 陈佳林, 周丽芳, 韦祎, 等. 右美托咪定对丙泊酚诱导新生大鼠大脑发育的远期影响[J]. 临床麻醉学杂志, 2018, 34(2): 163-166. DOI: 10.12089/jca.2018.02.015.

CHEN J L ZHOU L F WEI Y Long-term effects of dexmedetomidine on the brain development in propofol-induced neonatal rats J Clin Anesthesiol 2018 34 2 163 166 10.12089/jca.2018.02.015 CHEN J L, ZHOU L F, WEI Y, et al . Long-term effects of dexmedetomidine on the brain development in propofol-induced neonatal rats[J ] . J Clin Anesthesiol, 2018, 34(2): 163-166. DOI: 10.12089/jca.2018.02.015.

SUN DD WANG J H LIU X L Dexmedetomidine attenuates endoplasmic reticulum stress-induced apoptosis and improves neuronal function after traumatic brain injury in mice Brain Res 2020 1732 146682 10.1016/j.brainres.2020.146682 SUN DD, WANG J H, LIU X L, et al . Dexmedetomidine attenuates endoplasmic reticulum stress-induced apoptosis and improves neuronal function after traumatic brain injury in mice[J ] . Brain Res, 2020, 1732: 146682. DOI: 10.1016/j.b rainres.2020.146682.

DARLING N J COOK S J The role of MAPK signalling pathways in the response to endoplasmic reticulum stress Biochim Biophys Acta 2014 1843 10 2150 2163 10.1016/j.bbamcr.2014.01.009 DARLING N J, COOK S J. The role of MAPK signalling pathways in the response to endoplasmic reticulum stress[J]. Biochim Biophys Acta, 2014, 1843(10): 2150-2163. DOI: 10.1016/j.bbamcr.2014.01.009.

李红霞翁亦齐喻文立麻醉因素对亲体肝移植术患儿肾损伤的影响: 七氟醚复合麻醉与丙泊酚复合麻醉的比较中华麻醉学杂志 2019 39 3 340 342 10.3760/cma.j.issn.0254-1416.2019.03.022 李红霞, 翁亦齐, 喻文立, 等. 麻醉因素对亲体肝移植术患儿肾损伤的影响: 七氟醚复合麻醉与丙泊酚复合麻醉的比较[J]. 中华麻醉学杂志, 2019, 39(3): 340-342. DOI: 10.3760/cma.j.issn.0254-1416.2019.03.022.

LI H X WENG Y Q YU W L Effect of anesthesia factor on kidney injury in pediatric patients undergoing living donor liver trans-plantation: a comparison ofsevoflurane-versus propofol-based anesthesia Chin J Anesthesiol 2019 39 3 340 342 10.3760/cma.j.issn.0254-1416.2019.03.022 LI H X, WENG Y Q, YU W L, et al . Effect of anesthesia factor on kidney injury in pediatric patients undergoing living donor liver trans-plantation: a comparison ofsevoflurane-versus propofol-based anesthesia[J ] . Chin J Anesthesiol, 2019, 39(3): 340-342. DOI: 10.3760/cma.j.issn.0254- 1416.2019.03.022.

QIN J H ZHANG X R HE L Effect of sevoflurane and halothane anesthesia on cognitive function and immune function in young rats Saudi J Biol Sci 2018 25 1 47 51 10.1016/j.sjbs.2016.08.002 QIN J H, ZHANG X R, HE L, et al . Effect of sevoflurane and halothane anesthesia on cognitive function and immune function in young rats[J ] . Saudi J Biol Sci, 2018, 25(1): 47-51. DOI: 10.1016/j.sjbs.2016.08.002.

罗晶晶梁笑皮龙辉右美托咪定联合七氟醚吸入对单肺通气患者肺损伤的影响重庆医学 2019 48 11 1844 1847, 1851 10.3969/j.issn.1671-8348.2019.11.011 罗晶晶, 梁笑, 皮龙辉, 等. 右美托咪定联合七氟醚吸入对单肺通气患者肺损伤的影响[J]. 重庆医学2019, 48(11), 1844-1847, 1851. DOI: 10.3969/j.issn.1671-8348.2019.11.011.

LUO J J LIANG X PI L H Effect of dexmedetomidine combined with sevoflurane inhalation on lung injury in patients with one lung ventilation Chongqing Med 2019 48 11 1844 1847, 1851 10.3969/j.issn.1671-8348.2019.11.011.issn.1671-8348.2019.11.011 LUO J J, LIANG X, PI L H, et al . Effect of dexmedetomidine combined with sevoflurane inhalation on lung injury in patients with one lung ventilation [J ] . Chongqing Med, 2019, 48 (11): 1844-1847, 1851. DOI: 10.3969/j.issn.1671-8348.2019.11.011.issn.1671-8348.2019.11.011.

LIAO Z X CAO D X HAN X Both JNK and P38 MAPK pathways participate in the protection bydexmedetomidine against isoflurane-induced neuroapoptosis in the hippocampus of neonatal rats Brain Res Bull 2014 107 69 78 10.1016/j.brainresbull.2014.07.001 LIAO Z X, CAO D X, HAN X, et al . Both JNK and P38 MAPK pathways participate in the protection bydexmedetomidine against isoflurane-induced neuroapoptosis in the hippocampus of neonatal rats[J ] . Brain Res Bull, 2014, 107: 69-78. DOI: 10.1016/j.brainresbull. 2014.07.001.

Huang B Y Huang H B Zhang Z J Cell cycle activation contributes to isoflurane-induced neurotoxicity in the developing brain and the protective effect of CR8 CNS Neurosci Ther 2019 25 5 612 620 10.1111/cns.13090 Huang B Y, Huang H B, Zhang Z J, et al . Cell cycle activation contributes to isoflurane-induced neurotoxicity in the developing brain and the protective effect of CR8[J ] . CNS Neurosci Ther, 2019, 25(5): 612-620. DOI: 10.1111/cns. 13090.

LEBEAU J SAUNDERS J M MORAES V W R The PERK arm of the unfolded protein response regulates mitochondrial morphology during acute endoplasmic reticulum stress Cell Rep 2018 22 11 2827 2836 10.1016/j.celrep.2018.02.055 LEBEAU J, SAUNDERS J M, MORAES V W R, et al . The PERK arm of the unfolded protein response regulates mitochondrial morphology during acute endoplasmic reticulum stress[J ] . Cell Rep, 2018, 22(11): 2827-2836. DOI: 10.1016/j.celrep. 2018.02.055.

郭润民刘畅吴子君 p38MAPK通路激活内质网应激参与高糖诱导血管平滑肌细胞钙化广东医学院学报 2016 34 3 225 229 10.3969/j.issn.1005-4057.2016.03.002 郭润民, 刘畅, 吴子君, 等. p38MAPK通路激活内质网应激参与高糖诱导血管平滑肌细胞钙化[J]. 广东医学院学报, 2016, 34(3): 225-229. DOI: 10.3969/j.issn.1005-4057.2016. 03.002.

GUO R M LIU C WU Z J Involvement of p38 MAPK-activated endoplasmic reticulum stress in high glucose-induced calcification of vascular smooth muscular cells J Guangdong MedColl 2016 34 3 225 229 10.3969/j.issn.1005-4057.2016.03.002 GUO R M, LIU C, WU Z J, et al . Involvement of p38 MAPK-activated endoplasmic reticulum stress in high glucose-induced calcification of vascular smooth muscular cells[J ] . J Guangdong MedColl, 2016, 34(3): 225-229. DOI: 10.3969/j.issn.1005-4057.2016.03.002.

QIU Z G LU P WANG K Dexmedetomidine inhibits neuroinflammation by altering microglial M1/M2 polarization through MAPK/ERK pathway Neurochem Res 2020 45 2 345 353 10.1007/s11064-019-02922-1 QIU Z G, LU P, WANG K, et al . Dexmedetomidine inhibits neuroinflammation by altering microglial M1/M2 polarization through MAPK/ERK pathway[J ] . Neurochem Res, 2020, 45(2): 345-353. DOI: 10.1007/s11064-019- 02922-1.

WANG Q TAN Y H ZHANG N Dexmedetomidine inhibits activation of the MAPK pathway and protects PC12 and NG108-15 cells from lidocaine-induced cytotoxicity at its maximum safe dose Biomed Pharmacother 2017 91 162 166 10.1016/j.biopha.2017.04.084 WANG Q, TAN Y H, ZHANG N, et al . Dexmedetomidine inhibits activation of the MAPK pathway and protects PC12 and NG108-15 cells from lidocaine-induced cytotoxicity at its maximum safe dose[J ] . Biomed Pharmacother, 2017, 91: 162-166. DOI: 10.1016/j.biopha.2017.04.084.

KANG F C WANG S C CHANG MM Midazolam activates caspase, MAPKs and endoplasmic reticulum stress pathways, and inhibits cell cycle and Akt pathway, to induce apoptosis in TM3 mouse Leydig progenitor cells Oncotargets Ther 2018 11 1475 1490 10.2147/OTT.S154442 KANG F C, WANG S C, CHANG MM, et al . Midazolam activates caspase, MAPKs and endoplasmic reticulum stress pathways, and inhibits cell cycle and Akt pathway, to induce apoptosis in TM3 mouse Leydig progenitor cells[J ] . Oncotargets Ther, 2018, 11: 1475-1490. DOI: 10.2147/OTT.S154442.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

Toll样受体4抑制剂对新生大鼠缺氧缺血后脑海马神经元及树突棘的影响

右美托咪定对脓毒症大鼠血管内皮屏障功能的保护作用

苯并芘暴露大鼠海马组织的全基因组DNA甲基化差异分析

海洛因成瘾大鼠硫化氢/胱硫醚β-合酶体系的变化

氯胺酮对新生大鼠海马齿状回NMDA受体亚型表达的影响