骨髓源性生长因子对高糖诱导H9c2细胞焦亡的改善作用

张玉; 王艳; 赵然尊; 仇治梅; 龙仙萍; 李朝富; 陈文明; 石蓓

doi:10.16016/j.1000-5404.202011210

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骨髓源性生长因子对高糖诱导H9c2细胞焦亡的改善作用

基础医学 | 更新时间：2026-01-09

- 骨髓源性生长因子对高糖诱导H9c2细胞焦亡的改善作用
- Myeloid-derived growth factor improves pyroptosis in H9c2 cells induced by high glucose
- 第三军医大学学报 2021年43卷第10期页码：923-929
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金地区项目(81860061);国家自然科学基金地区科学基金(82060061);贵州省高层次创新型人才培养“百层次”;遵义医科大学附属医院硕士科研启动基金(院字(2016)51号)
- DOI：10.16016/j.1000-5404.202011210
  中图分类号：
- 收稿：2020-11-22，
  
  纸质出版：2021-05-30
- 稿件说明：
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张玉, 王艳, 赵然尊, 等. 骨髓源性生长因子对高糖诱导H9c2细胞焦亡的改善作用[J]. 第三军医大学学报, 2021,43(10):923-929.

Yu ZHANG, Yan WANG, Ranzun ZHAO, et al. Myeloid-derived growth factor improves pyroptosis in H9c2 cells induced by high glucose[J]. Journal of Third Military Medical University, 2021, 43(10): 923-929.
张玉, 王艳, 赵然尊, 等. 骨髓源性生长因子对高糖诱导H9c2细胞焦亡的改善作用[J]. 第三军医大学学报, 2021,43(10):923-929. DOI： 10.16016/j.1000-5404.202011210.

Yu ZHANG, Yan WANG, Ranzun ZHAO, et al. Myeloid-derived growth factor improves pyroptosis in H9c2 cells induced by high glucose[J]. Journal of Third Military Medical University, 2021, 43(10): 923-929. DOI： 10.16016/j.1000-5404.202011210.

摘要

Abstract

Objective

To investigate whether myeloid-derived growth factor (Mydgf) can improve cardiomyocyte pyroptosis induced by hyperglycemia by regulating nucleotide-binding domain-like receptor protein 3 (NLRP3).

Methods

Cultured rat cardiomyocyte H9c2 cells were divided into 4 groups: normal group (Nor)

high glucose group (HG

50 mmol/L glucose for 24 h)

Mydgf group (25 ng/mL recombinant Mydgf protein for 12 h

followed by 50 mmol/L glucose for 24 h)

and necrosulfonamide group (NSA

32 μmol/L necrosulfonamide for 0.5 h

followed by 50 mmol/L glucose for 24 h). Western blotting was used to detect the protein levels of cleaved Caspase-1

NLRP3

apoptosis-related speckle-like proteins (ACS) and gasdermin D (GSDMD). The secretions of IL-1β and IL-18 in cell culture medium were detected by ELISA. CCK-8 assay was used to detect the effect of Mydgf or NLRP3 on the viability of H9c2 cells. Lentiviral vector carrying NLRP3 and enhanced green fluorescent protein (EGFP) (NLRP3-EGFP) and empty viral vector (EGFP) were transfected into H9c2 cells respectively

and the changes of pyroptosis related indexes were detected by recovery experiments.

Results

Western blot results showed that the expression levels of pyroptosis related proteins

cleaved Caspase 1

ACS

NLRP3 and GSDMD were significantly higher in the HG group than the Nor group (

0.05). Mydgf treatment effectively inhibited the expression of a

bove proteins (

0.05)

but the inhibitory efficiencies were lower than that in the NSA group (

0.05). ELISA demonstrated that the secretions of IL-1β and IL-18 were increased in the HG group (

0.05)

but decreased in the Mydgf group (

0.05)

and more significantly reduced in the NSA group (

0.05). CCK-8 assay indicated that Mydgf at a concentration of below 25 ng/mL had no significant effect on the viability of H9c2 cells

while when the concentration above 25 ng/mL

the treatment significantly decreased the cell viability (

0.05). Compared with the control group

overexpression of NLRP3 significantly decreased H9c2 cells viability under the condition of HG. Transfection of lentiviral vector NLRP3-EGFP resulted in obvious increases in the mRNA and protein expression of NLRP3 (

0.05)

but that of empty vector had no such effects (

0.05). In the recovery experiment

Western blot results showed that in the high-glucose environment

the expression level of NLRP3

ASC

and cleaved Caspase-1 were significantly increased (

0.05)

and the secretions of IL-1β and IL-18 were also significantly enhanced in the Mydgf +NLRP3-EGFP group compared with the Mydgf group (

0.05).

Conclusion

Mydgf may ameliorate cardiomyocyte pyroptosis induced by hyperglycemia by inhibiting NLRP3.

关键词

Keywords

references

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