HBx介导SAMHD1降解并通过Akt/p27通路促进肝癌细胞增殖的机制研究

郑小川; 皮思蝶; 胡源

doi:10.16016/j.1000-5404.202011188

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HBx介导SAMHD1降解并通过Akt/p27通路促进肝癌细胞增殖的机制研究

本期推荐：HBx在肝癌发病中的作用 | 更新时间：2026-01-09

- HBx介导SAMHD1降解并通过Akt/p27通路促进肝癌细胞增殖的机制研究
- Mechanism of hepatitis B virus X mediating SAMHD1 degradation to promote proliferation of hepatoma cells through AKT/p27 pathway
- 第三军医大学学报 2021年43卷第10期页码：900-907
- 作者机构：
- 作者简介：
- 基金信息：
  
  重庆市渝中区科技项目(20200122)
- DOI：10.16016/j.1000-5404.202011188
  中图分类号：
- 收稿：2020-11-20，
  
  纸质出版：2021-05-30
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郑小川, 皮思蝶, 胡源. HBx介导SAMHD1降解并通过Akt/p27通路促进肝癌细胞增殖的机制研究[J]. 第三军医大学学报, 2021,43(10):900-907.

Xiaochuan ZHENG, Sidie PI, Yuan HU. Mechanism of hepatitis B virus X mediating SAMHD1 degradation to promote proliferation of hepatoma cells through AKT/p27 pathway[J]. Journal of Third Military Medical University, 2021, 43(10): 900-907.
郑小川, 皮思蝶, 胡源. HBx介导SAMHD1降解并通过Akt/p27通路促进肝癌细胞增殖的机制研究[J]. 第三军医大学学报, 2021,43(10):900-907. DOI： 10.16016/j.1000-5404.202011188.

Xiaochuan ZHENG, Sidie PI, Yuan HU. Mechanism of hepatitis B virus X mediating SAMHD1 degradation to promote proliferation of hepatoma cells through AKT/p27 pathway[J]. Journal of Third Military Medical University, 2021, 43(10): 900-907. DOI： 10.16016/j.1000-5404.202011188.

摘要

Abstract

Objective

To investigate the molecular mechanism of hepatitis B virus X (HBx) regulating the degradation of SAMHD1 and promoting the proliferation of hepatocellular carcinoma (HCC) cells.

Methods

The SAMHD1 expression in hepatoma and para-cancerous tissues collected from 15 HCC patients was detected by Western blotting. A stable hepatocellular carcinoma cell line of HepAD38 with SAMHD1 knockout was constructed using CRISP9/Cas9 technology

and the effects of SAMHD1 knockout on the change of proliferation and cell cycle were measured by MTT assay and flow cytometry

respectively

and its effects on related cyclin expression and Akt/p27 pathway were also detected by Western blotting. The impacts of HBx protein overexpression on the mRNA and protein expression of SAMHD1 were subsequently examined. In addition

the molecular mechanism of HBx regulating the degradation of SAMHD1 was further investigated through ubiquitin-proteasome pathway using cycloheximide (CHX) and MG132 treatment along with immunoprecipitation (IP) analysis.

Results

① The expression of SAMHD1 was significantly lower in liver cancer tissues than the para-cancerous tissues (

0.01).②A stable SAMHD1 knockout cell line HepAD38 (KO-SAMHD1) was successfully constructed

and MTT assay showed that the KO-SAMHD1 group had obviously faster cell proliferation (

0.01)

and flow cytometry indicated the group had larger amounts of cells arrested in G

/M phase when compared with the control group. ③Western blot analysis indicated that the expression of related cyclins was changed in the KO-SAMHD1 group when compared with the control group

including the expression of p27 protein decreased and p-Akt protein increased. ④

In vitro

cell experiments showed that HBx overexpression induced decreased expression of SAMHD1 in Huh7.0 cells. ⑤The results of immunoprecipitation suggested that HBx were combined and interacted with SAMHD1. CHX and ubiquitination IP assay indicated an earlier decay time of SAMHD1 protein and enhanced ubiquitination of SAMHD1 induced by HBx.

Conclusion

HBx interacts with SAMHD1 and degrades it by regulating its ubiquitination

and thereby affects the Akt/p27 pathway to modulate the cell cycle and promote the proliferation of HCC cells.

关键词

Keywords

references

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