HBx通过COX-2及其下游因子诱导小鼠发生肝癌的机制研究

毋楠; 周梦瑶; 杜彬; 黄欣; 张思遥; 况钦; 吴勇; 冯涛

doi:10.16016/j.1000-5404.202011103

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HBx通过COX-2及其下游因子诱导小鼠发生肝癌的机制研究

本期推荐：HBx在肝癌发病中的作用 | 更新时间：2026-01-09

- HBx通过COX-2及其下游因子诱导小鼠发生肝癌的机制研究
- Mechanism of hepatitis B virus X inducing hepatocellular carcinoma in mice through COX-2 and its downstream factors
- 第三军医大学学报 2021年43卷第10期页码：892-899
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81071770)
- DOI：10.16016/j.1000-5404.202011103
  中图分类号：
- 收稿：2020-11-11，
  
  纸质出版：2021-05-30
- 稿件说明：
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毋楠, 周梦瑶, 杜彬, 等. HBx通过COX-2及其下游因子诱导小鼠发生肝癌的机制研究[J]. 第三军医大学学报, 2021,43(10):892-899.

Nan WU, Mengyao ZHOU, Bin DU, et al. Mechanism of hepatitis B virus X inducing hepatocellular carcinoma in mice through COX-2 and its downstream factors[J]. Journal of Third Military Medical University, 2021, 43(10): 892-899.
毋楠, 周梦瑶, 杜彬, 等. HBx通过COX-2及其下游因子诱导小鼠发生肝癌的机制研究[J]. 第三军医大学学报, 2021,43(10):892-899. DOI： 10.16016/j.1000-5404.202011103.

Nan WU, Mengyao ZHOU, Bin DU, et al. Mechanism of hepatitis B virus X inducing hepatocellular carcinoma in mice through COX-2 and its downstream factors[J]. Journal of Third Military Medical University, 2021, 43(10): 892-899. DOI： 10.16016/j.1000-5404.202011103.

摘要

Abstract

Objective

To investigate the dynamic changes and the roles of COX-2 and its downstream factors in the carcinogenic process using a mouse model stably expressing hepatitis B virus X (HBx).

Methods

The expression of HBx in HBx-EGFP-14-19 cells was detected by Western blotting and RT-qPCR. A mouse model with stable expression of HBx was then established by injecting HBx-EGFP-14-19 cells into KM mice

via

hepatic portal vein. The mice were subsequently sacrificed at 30

180 and 360 d

respectively

and the liver tissues were collected. Western blotting

RT-qPCR and immunohistochemical assay were used to detect the expression of HBx in the liver; HE staining was adopted to observe the pathological characteristics of liver tissues. In addition

Western blotting and RT-qPCR were also employed to determine the expression changes of COX-2 and its downstream factors

cell cycle- and apoptosis-related factors in the liver tissues

at both mRNA and protein levels.

Results

The animal model with long-term stable expression of HBx was successfully constructed and eventually developed into liver cancer. The mRNA and protein levels of COX-2 and its downstream factor VEGF were continuously up-regulated even in 90 d after HBx was transferred into mice and the increment was continued until tumorigenesis (

0.05). The protein and mRNA levels of liver pro-apoptotic factors Bad and Bax were decreased (

0.05)

while those of the anti-apoptotic factor Bcl-2 were increased (

0.05)

and the levels of cell cycle factors Cyclin D1 and CDK4 were increased (

0.05). However

the effects of HBx on the above genes were reversed after administration of COX-2 inhibitor celecoxib.

Conclusion

HBx can cause liver cancer

which may be related to the up-regulation of COX-2 and its downstream factors.

关键词

Keywords

references

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