NGF、BDNF和Netrin-1在弥漫大B细胞淋巴瘤中的表达

蒲欣; 张勇; 陈文; 杨再林; 杨赛男; 魏霞

doi:10.16016/j.1000-5404.202010172

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NGF、BDNF和Netrin-1在弥漫大B细胞淋巴瘤中的表达

临床医学 | 更新时间：2026-01-09

- NGF、BDNF和Netrin-1在弥漫大B细胞淋巴瘤中的表达
- Expression of NGF, BDNF and Netrin-1 in diffuse large B cell lymphoma
- 第三军医大学学报 2021年43卷第8期页码：715-721
- 作者机构：
  
  401120 重庆，重庆医科大学附属第三医院(捷尔医院)血液科
- 作者简介：
  
  [魏霞，E-mail: 10988992@qq.com WEI Xia, E-mail: 10988992@qq.com
- 基金信息：
  
  国家自然科学基金青年科学基金(31800814)
- DOI：10.16016/j.1000-5404.202010172
  中图分类号： R341;R730.23;R733.4
- 收稿：2020-10-26，
  
  修回：2021-01-04，
  
  纸质出版：2021-04-30
- 稿件说明：
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蒲欣, 张勇, 陈文, 等. NGF、BDNF和Netrin-1在弥漫大B细胞淋巴瘤中的表达[J]. 第三军医大学学报, 2021,43(8):715-721.

Xin PU, Yong ZHANG, Wen CHEN, et al. Expression of NGF, BDNF and Netrin-1 in diffuse large B cell lymphoma[J]. Journal of Third Military Medical University, 2021, 43(8): 715-721.
蒲欣, 张勇, 陈文, 等. NGF、BDNF和Netrin-1在弥漫大B细胞淋巴瘤中的表达[J]. 第三军医大学学报, 2021,43(8):715-721. DOI： 10.16016/j.1000-5404.202010172.

Xin PU, Yong ZHANG, Wen CHEN, et al. Expression of NGF, BDNF and Netrin-1 in diffuse large B cell lymphoma[J]. Journal of Third Military Medical University, 2021, 43(8): 715-721. DOI： 10.16016/j.1000-5404.202010172.

摘要

目的

探讨神经生长因子(nerve growth factor，NGF)、脑源性神经生长因子(brain-derived neurotrophic factor，BDNF)和Netrin-1在弥漫大B细胞淋巴瘤中的表达。

方法

收集2018年5月至2020年5月来重庆医科大学附属第三医院就诊的弥漫大B细胞淋巴瘤初治患者；按照淋巴瘤国际预后指数(international prognostic index，IPI)将患者分成低危组(评分≤2)和高危组(评分＞2)，每组25例患者。免疫组化检测Ki-67、NGF、BDNF和Netrin-1的表达；流式细胞术检测调节性T细胞(regulatory T cell，Treg细胞)、调节性B细胞(regulatory B cell，Breg细胞)和调节性浆细胞(regulatory plasma cell，Preg细胞)的比例。

结果

相对于低危组，高危组Ki-67、NGF和Netrin-1的表达均显著升高(

0.05)，而BDNF的表达则没有显著的改变；相对于低危组，高危组外周血Treg细胞比例显著升高(3.07±0.14

3.72±0.18，

0.05)，而Breg和Preg细胞的比例则没有显著的改变(0.88±0.12

1.06± 0.10；0.053±0.010

0.054±

0.009)；相关性分析结果显示NGF与Ki-67增殖指数呈正相关性(

=0.720，

=0.008)，而与Treg、Breg和Preg细胞比例均不具有相关性；Netrin-1与Ki-67增殖指数、Treg细胞比例均呈正相关性(

=0.804，

=0.002；

=0.608，

=0.036)，而与Breg和Preg细胞比例均不具有相关性；BDNF与Ki-67增殖指数、Treg细胞、Breg细胞和Preg细胞比例均不具有相关性。

结论

NGF和Netrin-1在弥漫大B细胞淋巴瘤中表达升高，且与其增殖水平和外周血Treg细胞比例相关。

Abstract

Objective

To investigate the expression of nerve growth factor (NGF)

brain-derived neurotrophic factor (BDNF) and Netrin-1 in diffuse large B cell lymphoma (DLBCL).

Methods

The patients with freshly-diagnosed DLBCL in our hospital from May 2018 to May 2020 were enrolled in this study. According to the international prognostic index (IPI)

they were divided into low-risk group (≤2 points) and high-risk group (>2 points)

with 25 patients in each group. The expression of Ki-67

NGF

BDNF and Netrin-1 were detected by immunohistochemical assay. Flow cytometry was used to detect the proportions of regulatory T cells (Treg cells)

regulatory B cells (Breg cells) and regulatory plasma cells (Preg cells).

Results

Compared with the low-risk group

the expression levels of Ki-67

NGF and Netrin-1 were significantly increased in the high-risk group (

0.05)

but that of BDNF didn't differ. The proportion of Treg cells in peripheral blood was obviously increased (3.07±0.14

3.72±0.18

0.05)

while those of Breg cells and Preg cells were not statistically changed (0.88±0.12

1.06±0.10; 0.053±0.010

0.054±0.009) in the high-risk group than the low-risk group. Correlation analysis results showed that NGF was positively correlated with Ki-67 proliferation index (

=0.720

=0.008)

but not with the proportions of Treg cells

Breg cells and Preg cells. Netrin-1 was positively correlated with Ki-67 proliferation index and proportion of Treg cells (

=0.804

=0.002;

=0.608

=0.036)

but not with the proportions of Breg cells and Preg cells. There was no correlation of BDNF with Ki-67 proliferation index and above proportions.

Conclusion

The expression levels of NGF and Netrin-1 are significantly increased in DLBCL tissues and positively correlated with the proliferation of tumor cells and proportion of Treg cells.

关键词

Keywords

references

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