抑郁症模型大鼠海马内小胶质细胞数量和炎症因子表达增加

肖凯; 罗艳敏; 梁芯; 唐静; 王瑾; 肖倩; 綦英强; 黎悦; 祝佩林; 杨浩; 谢雨涵; 唐勇; 吴宏

doi:10.16016/j.1000-5404.202010091

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抑郁症模型大鼠海马内小胶质细胞数量和炎症因子表达增加

神经科学 | 更新时间：2026-01-09

- 抑郁症模型大鼠海马内小胶质细胞数量和炎症因子表达增加
- Increased number of microglia and enhanced expression of inflammatory factors in the hippocampus of rat model of depression
- 第三军医大学学报 2021年43卷第5期页码：367-375
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81871073)
- DOI：10.16016/j.1000-5404.202010091
  中图分类号：
- 收稿：2020-10-15，
  
  纸质出版：2021-03-15
- 稿件说明：
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肖凯, 罗艳敏, 梁芯, 等. 抑郁症模型大鼠海马内小胶质细胞数量和炎症因子表达增加[J]. 第三军医大学学报, 2021,43(5):367-375.

Kai XIAO, Yanmin LUO, Xin LIANG, et al. Increased number of microglia and enhanced expression of inflammatory factors in the hippocampus of rat model of depression[J]. Journal of Third Military Medical University, 2021, 43(5): 367-375.
肖凯, 罗艳敏, 梁芯, 等. 抑郁症模型大鼠海马内小胶质细胞数量和炎症因子表达增加[J]. 第三军医大学学报, 2021,43(5):367-375. DOI： 10.16016/j.1000-5404.202010091.

Kai XIAO, Yanmin LUO, Xin LIANG, et al. Increased number of microglia and enhanced expression of inflammatory factors in the hippocampus of rat model of depression[J]. Journal of Third Military Medical University, 2021, 43(5): 367-375. DOI： 10.16016/j.1000-5404.202010091.

摘要

Abstract

Objective

To investigate the changes of the total microglial number

density of activated microglia and inflammatory cytokines in the hippocampus of rat model of depression induced by chronic unpredictable stress (CUS).

Methods

Male Sprague-Dawley (SD) rats (6~8 weeks old) were randomly divided into control group (

=19) and CUS intervention group (

=48) with stratified randomization after adaptive feeding

sucrose preference baseline adjustment and screening. The rats of CUS intervention group were subjected to CUS intervention for 5 weeks. The depressive-like symptoms of 2 groups of rats were assessed with the sucrose preference test

and 16 model rats were screened out according to the results of sucrose preference. Immunohistochemical assay and stereological method were used to quantify the total number of microglia in the hippocampus of 2 groups of rats. The density of activated microglia in the hippocampus were studied with immunofluorescence assay. The contents of inflammatory cytokines in the hippocampus were measured with quantitative real-time polymerase chain reaction (qRT-PCR).

Results

The body mass and percentage of sucrose preference were significantly lower in the CUS model group than in the control group (

0.01). The numbers of the microglia in the hippocampal CA1

CA2/3 and DG regions were significantly increased in the CUS model group compared with the control group (

0.001

=0.002

=0.002)

so were the density

and proportion of the activated microglia in the CA1

CA2/3 and DG regions (

=0.011

=0.015

0.001;

=0.018

=0.019

0.001). Compared with the control group

the mRNA levels of inflammatory cytokines IL-1β and iNOS in the hippocampus were obviously higher in the model group (

=0.012

=0.020).

Conclusion

The number of the microglia

density and proportion of activated microglia as well as levels of inflammatory cytokines are increased in the hippocampus of depression rats

indicating that the changes of hippocampal microglia might be involved in the pathogenesis of depression.

关键词

Keywords

references

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