环状RNA circVCAN对胎盘滋养细胞增殖、侵袭及自噬的影响

袁小岚; 刘丽; 张嫘; 唐代钰; 杨童; 董晓静

doi:10.16016/j.1000-5404.202010070

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环状RNA circVCAN对胎盘滋养细胞增殖、侵袭及自噬的影响

基础医学 | 更新时间：2026-01-09

- 环状RNA circVCAN对胎盘滋养细胞增殖、侵袭及自噬的影响
- Effects of circular RNA circVCAN on proliferation, invasion and autophagy of placental trophoblast cells
- 第三军医大学学报 2021年43卷第6期页码：489-495
- 作者机构：
- 作者简介：
- 基金信息：
  
  重庆市自然科学基金(cstc2019jcyj-msxmX0318);教育部生殖与发育国际合作联合实验室及高等学校学科创新引智计划
- DOI：10.16016/j.1000-5404.202010070
  中图分类号：
- 收稿：2020-10-14，
  
  纸质出版：2021-03-30
- 稿件说明：
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袁小岚, 刘丽, 张嫘, 等. 环状RNA circVCAN对胎盘滋养细胞增殖、侵袭及自噬的影响[J]. 第三军医大学学报, 2021,43(6):489-495.

Xiaolan YUAN, Li LIU, Lei ZHANG, et al. Effects of circular RNA circVCAN on proliferation, invasion and autophagy of placental trophoblast cells[J]. Journal of Third Military Medical University, 2021, 43(6): 489-495.
袁小岚, 刘丽, 张嫘, 等. 环状RNA circVCAN对胎盘滋养细胞增殖、侵袭及自噬的影响[J]. 第三军医大学学报, 2021,43(6):489-495. DOI： 10.16016/j.1000-5404.202010070.

Xiaolan YUAN, Li LIU, Lei ZHANG, et al. Effects of circular RNA circVCAN on proliferation, invasion and autophagy of placental trophoblast cells[J]. Journal of Third Military Medical University, 2021, 43(6): 489-495. DOI： 10.16016/j.1000-5404.202010070.

摘要

Abstract

Objective

To investigate the expression

function and potential mechanism of circular RNA circVCAN(also known as hsa-circ-0073239)in fetal growth restriction (FGR) placenta.

Methods

The placenta tissue samples were collected from 24 pregnant women (12 with FGR and 12 normal pregnancy) admitted in our hospital from January to December 2018. Real-time quantitative PCR (RT-qPCR) was performed to detect the expression of circVCAN in these placenta samples. Human placental trophoblast cell line HTR8/SVneo was cultured and then transfected with circVCAN interference and overexpression plasmid

respectively. EdU assay

flow cytometer and Transwell assay were carried out respectively to verify the variations in the proliferation

apoptosis and invasion abilities of the cells. And the expression of autophagy related proteins LC3Ⅱ/LC3Ⅰ

ATG5 and ATG7 was measured by Western blotting.

Results

The expression levels of circVCAN

ATG5

ATG7 and LC3Ⅱ/LC3Ⅰwere significantly higher in FGR placenta tissue than in the normal group (

0.01). The termination of pregnancy was earlier in FGR patients than in normal group (

0.05)

with newborns from the FGR group having lower birth body weight (

0.01) and placenta weight (

0.05) as well as shorter birth body length (

0.01) when compared with those from the normal group. Silencing of circVCAN resulted in enhanced proliferation and invasion abilities in HTR8/SVneo cells (

0.01)

while circVCAN overexpression led to decreased proliferation and invasion abilities in the cells (

0.01)

but no statistical difference was seen in the apoptotic rate (

0.05). The expression levels of autophagy related proteins LC3Ⅱ/LC3Ⅰ

ATG5 and ATG7 were significantly lower in circVCAN interfered cells (

0.05)

while higher in circVCAN overexpressed cells (

0.05).

Conclusion

CircVCAN is highly expressed in FGR placenta tissue

though it showing no effect on apoptosis but inhibiting the proliferation and invasion of HTR8/SVneo trophoblast cells

and may enhance autophagy by promoting the expression of ATG5 and ATG7.

关键词

Keywords

references

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