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NPRL2与膀胱癌患者临床病理特征的相关性及对膀胱癌细胞凋亡的影响
Correlation of NPRL2 with cinicopathological features of bladder cancer and its effect on cell apoptosis
- 第三军医大学学报 2021年43卷第5期 页码：432-437
- 作者机构：
- 作者简介：
- 基金信息：
DOI：10.16016/j.1000-5404.202009233
中图分类号：收稿：2020-09-27，
纸质出版：2021-03-15
- 稿件说明：
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朱西, 罗生军, 蒋立, 等. NPRL2与膀胱癌患者临床病理特征的相关性及对膀胱癌细胞凋亡的影响[J]. 第三军医大学学报, 2021,43(5):432-437.
Xi ZHU, Shengjun LUO, Li JIANG, et al. Correlation of NPRL2 with cinicopathological features of bladder cancer and its effect on cell apoptosis[J]. Journal of Third Military Medical University, 2021, 43(5): 432-437.
朱西, 罗生军, 蒋立, 等. NPRL2与膀胱癌患者临床病理特征的相关性及对膀胱癌细胞凋亡的影响[J]. 第三军医大学学报, 2021,43(5):432-437. DOI： 10.16016/j.1000-5404.202009233.
Xi ZHU, Shengjun LUO, Li JIANG, et al. Correlation of NPRL2 with cinicopathological features of bladder cancer and its effect on cell apoptosis[J]. Journal of Third Military Medical University, 2021, 43(5): 432-437. DOI： 10.16016/j.1000-5404.202009233.
摘要
Abstract
Objective
2
To investigate the correlation between expression of NPRL2 and clinicopathological features of bladder cancer and the effect of NPRL2 silencing on the apoptosis of bladder cancer cell line Biu87/RT-4.
Methods
2
Cancer tissue samples were collected from 131 patients with bladder cancer admitted into our department from January 2014 to January 2019. The expression of NPRL2 in bladder cancer tissues was detected by immunohistochemistry
and the correlation between NPRL2 and the clinicopathological features of bladder cancer was analyzed. The expression of NPRL2 in CP-H068
Biu87 and RT-4 cell lines was detected
and the cell proliferation after NPRL2 silencing was detected by CCK-8 assay. Flow cytometry was used to detect cell apoptosis
and Western blotting to detect the expression of apoptosis-related proteins.
Results
2
NPRL2 was highly expressed in bladder cancer samples
accounting for 53.44%
and the expression was significantly correlated with the clinical stage and grade of bladder cancer (
P
<
0.05). Its expression was also obviously higher in the bladder cancer Biu87 and RT-4 cells than the normal bladder epithelial CP-H068 cells (
P
<
0.05). NPRL2 silencing resulted in decreased proliferation ability
elevated apoptotic rate
and increased protein levels of JNK
Bax and caspase-9
while decreased level of Bcl-2 in the Biu87 and RT-4 cells (
P
<
0.05).
Conclusion
2
The expression of NPRL2 is related to the clinical stage and grade of bladder cancer. Silencing NPRL2 in bladder cancer cells Biu87/RT-4 can effectively inhibit the proliferation and promote apoptosis of bladder cancer cells.
关键词
Keywords
references
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