重庆市大气细颗粒物中多环芳烃组分与男性精子DNA损伤的关联研究

王芙蓉; 周妮娅; 陈卿; 刘太秀; 曹佳; 张爱华

doi:10.16016/j.1000-5404.202009206

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重庆市大气细颗粒物中多环芳烃组分与男性精子DNA损伤的关联研究

专题报道:生殖损害和不良妊娠结局的危险因素研究 | 更新时间：2026-01-09

- 重庆市大气细颗粒物中多环芳烃组分与男性精子DNA损伤的关联研究
- Association between fine particulate matter bound polycyclic aromatic hydrocarbons and DNA damage of male sperm in Chongqing
- 第三军医大学学报 2021年43卷第1期页码：17-24
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金重点项目(81630087);国家重点研发计划项目(2017YFC1002001)
- DOI：10.16016/j.1000-5404.202009206
  中图分类号：
- 收稿：2020-09-24，
  
  纸质出版：2021-01-15
- 稿件说明：
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王芙蓉, 周妮娅, 陈卿, 等. 重庆市大气细颗粒物中多环芳烃组分与男性精子DNA损伤的关联研究[J]. 第三军医大学学报, 2021,43(1):17-24.

Furong WANG, Niya ZHOU, Qing CHEN, et al. Association between fine particulate matter bound polycyclic aromatic hydrocarbons and DNA damage of male sperm in Chongqing[J]. Journal of Third Military Medical University, 2021, 43(1): 17-24.
王芙蓉, 周妮娅, 陈卿, 等. 重庆市大气细颗粒物中多环芳烃组分与男性精子DNA损伤的关联研究[J]. 第三军医大学学报, 2021,43(1):17-24. DOI： 10.16016/j.1000-5404.202009206.

Furong WANG, Niya ZHOU, Qing CHEN, et al. Association between fine particulate matter bound polycyclic aromatic hydrocarbons and DNA damage of male sperm in Chongqing[J]. Journal of Third Military Medical University, 2021, 43(1): 17-24. DOI： 10.16016/j.1000-5404.202009206.

摘要

Abstract

Objective

To investigate the association between exposure to polycyclic aromat

ic hydrocarbons(PAHs) in atmospheric fine particulate matter(PM

2.5

) and sperm DNA damage in Chongqing.

Methods

The subjects were sampled from our previously established cohort of the male reproductive health in Chongqing college students(MARHCS) study. The concentrations of PAHs(collected during the sampling periods) with low fused aromatic rings(2~4 rings) and with high fused aromatic rings(5~6 rings) in the air PM

2.5

filter membranes were detected. Single cell gel electrophoresis(Comet assay) was used to evaluate DNA damage. Finally

the mixed-effects model was used to investigate the relationships between the exposure concentrations of PAHs components in PM

2.5

and severity of sperm DNA damage.

Results

In 2013

the low molecular weight(LMW) PAHs accounted for a large proportion in atmospheric fine particles

mainly phenanthrene

fluorene and pyrene. While

the high molecular weight(HMW) PAHs dominated in 2014

such as indeno[1

3-cd

]

pyrene

benzo[g

]

perylene and benzo[a

]

pyrene. Sperm DNA damage indicators

such as tail length

tail moment and Olive tail moment were more common in the cohort in the year of 2014 than in 2013(all

0.05). The results of the mixed model showed that the concentrations of fluoranthene

acenaphthene

pyrene and ∑LMW PAHs were negatively correlated with tail length and tail moment. The concentrations of benzo[k

]

fluoranthene

benzo[b

]

fluoranthene and dibenz[a

]

anthracene were positively correlated with tail length [

(95%

): 108.987(43.754~203.820)

158.700(65.957~303.272)

185.708(67.350~387.775)

respectively; all corrected

0.05

]

and with tail moment [

(95%

): 449.204(260.066~737.695)

683.108(374.515~1192.387)

683.108(374.515~1 192.387)

respectively; all corrected

0.05

]

Conclusion

The exposure of HMW PAHs in Chongqing atmospheric fine particles may lead to the enhancement of sperm DNA damage.

关键词

Keywords

references

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