KIF3A通过结合Wnt/β-catenin通路中的β-arrestin参与哮喘气道上皮的炎症调控

杨丽丽; 王亚苹; 薛坤娇; 阮玲瑛; 符州; 耿刚

doi:10.16016/j.1000-5404.202009180

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KIF3A通过结合Wnt/β-catenin通路中的β-arrestin参与哮喘气道上皮的炎症调控

基础医学 | 更新时间：2026-01-09

- KIF3A通过结合Wnt/β-catenin通路中的β-arrestin参与哮喘气道上皮的炎症调控
- Kinesin family member 3A is involved in inflammatory regulation of asthmatic airway epithelial by binding to β-arrestin in the Wnt/β-catenin pathway
- 第三军医大学学报 2021年43卷第6期页码：496-502
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81670018);重庆市教委科学技术研究项目(KJQN201800407)
- DOI：10.16016/j.1000-5404.202009180
  中图分类号：
- 收稿：2020-09-21，
  
  纸质出版：2021-03-30
- 稿件说明：
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杨丽丽, 王亚苹, 薛坤娇, 等. KIF3A通过结合Wnt/β-catenin通路中的β-arrestin参与哮喘气道上皮的炎症调控[J]. 第三军医大学学报, 2021,43(6):496-502.

Lili YANG, Yaping WANG, Kunjiao XUE, et al. Kinesin family member 3A is involved in inflammatory regulation of asthmatic airway epithelial by binding to β-arrestin in the Wnt/β-catenin pathway[J]. Journal of Third Military Medical University, 2021, 43(6): 496-502.
杨丽丽, 王亚苹, 薛坤娇, 等. KIF3A通过结合Wnt/β-catenin通路中的β-arrestin参与哮喘气道上皮的炎症调控[J]. 第三军医大学学报, 2021,43(6):496-502. DOI： 10.16016/j.1000-5404.202009180.

Lili YANG, Yaping WANG, Kunjiao XUE, et al. Kinesin family member 3A is involved in inflammatory regulation of asthmatic airway epithelial by binding to β-arrestin in the Wnt/β-catenin pathway[J]. Journal of Third Military Medical University, 2021, 43(6): 496-502. DOI： 10.16016/j.1000-5404.202009180.

摘要

Abstract

Objective

To investigate the possible mechanism of kinesin family member 3A (KIF3A) taking part in the regulation of asthma airway inflammation

so as to provide a new target and direction for asthma treatment.

Methods

Female C57BL/6 mice were randomly divided into asthma group (

=10) and control group (

=10). Western blotting and immunohistochemistry (IHC) were performed to detect the expression of KIF3A in the 2 groups. After human bronchial epithelial 16HBE cells were intervened using phosphate buffer solution (PBS) and house dust mite (HDM)

Western blotting and RT-PCR were adopted to detect the KIF3A expression. Moreover

the lentiviral vectors of KIF3A overexpression (LV-KIF3A-45894-J2

LV-KIF3A) and knock-down (LV-KIF3A-RNAi-81183-1

RNAi) were constructed and then transfected into 16HBE cells

and the blank lentiviral vectors and those containing negative control sequence served as controls

respectively. The binding of KIF3A to β-arrestin in the overexpressed cells was detected by immunoprecipitation

while the expression level of β-catenin in each group of cells was determined by Western blotting and RT-PCR. The effect of differential expression of KIF3A on the expression of chemokines CCL-17 and CCL-26 was also determined.

Results

In animal models

the expression of KIF3A was significantly lower in the asthma mice than the control mice (

0.05).

In vitro

study indicated that HDM intervention decreased the expression of KIF3A at protein and mRNA levels in 16HBE cells

though there was no statistical difference (

0.05). The RT-PCR and Western blotting results showed that the expression of KIF3A were increased in the LV-KIF3A transfected cells (

0.05) and decreased in the RNAi treated cells (

0.05). Immunoprecipitation confirmed the binding of KIF3A to β-arrestin. No significant difference was fou

nd in the total amount of β-catenin protein between the LV-KIF3A transfected cells and the RNAi treated cells. Compared with the corresponding cells

the mRNA level of β-catenin was significantly lower in the RNAi treated cells (

0.0001)

but no such difference was seen in the LV-KIF3A transfected cells. The RT-PCR results suggested that the levels of chemokines CCL-17 and CCL-26 were increased in the RNAi treated cells (

0.001)

while decreased in the LV-KIF3A transfected cells (

0.05) as compared with their control cells.

Conclusion

KIF3A binds to β-arrestin in the Wnt/β-catenin pathway

and is involved in asthmatic airway epithelial inflammation by regulating the expression of chemokines CCL-17 and CCL-26.

关键词

Keywords

references

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