基于TMT和PRM技术的湿性年龄相关性黄斑变性泪液蛋白质组学研究

覃珂; 刘丹宁; 周希瑗

doi:10.16016/j.1000-5404.202009170

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基于TMT和PRM技术的湿性年龄相关性黄斑变性泪液蛋白质组学研究

基础医学 | 更新时间：2026-01-09

- 基于TMT和PRM技术的湿性年龄相关性黄斑变性泪液蛋白质组学研究
- Tear proteomics in neovascular age-related macular degeneration based on TMT and PRM techniques
- 第三军医大学学报 2021年43卷第6期页码：473-480
- 作者机构：
- 作者简介：
- 基金信息：
  
  重庆市科学技术委员会(cstc2016jcyjA0122)
- DOI：10.16016/j.1000-5404.202009170
  中图分类号：
- 收稿：2020-09-20，
  
  纸质出版：2021-03-30
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覃珂, 刘丹宁, 周希瑗. 基于TMT和PRM技术的湿性年龄相关性黄斑变性泪液蛋白质组学研究[J]. 第三军医大学学报, 2021,43(6):473-480.

Ke QIN, Danning LIU, Xiyuan ZHOU. Tear proteomics in neovascular age-related macular degeneration based on TMT and PRM techniques[J]. Journal of Third Military Medical University, 2021, 43(6): 473-480.
覃珂, 刘丹宁, 周希瑗. 基于TMT和PRM技术的湿性年龄相关性黄斑变性泪液蛋白质组学研究[J]. 第三军医大学学报, 2021,43(6):473-480. DOI： 10.16016/j.1000-5404.202009170.

Ke QIN, Danning LIU, Xiyuan ZHOU. Tear proteomics in neovascular age-related macular degeneration based on TMT and PRM techniques[J]. Journal of Third Military Medical University, 2021, 43(6): 473-480. DOI： 10.16016/j.1000-5404.202009170.

摘要

Abstract

Objective

To investigate the tear proteomics of treatment-naïve eyes with naïve neovascular age-related macular degeneration (nAMD) and screen differentially expressed proteins (DEPs) so as to identify potential biomarkers.

Methods

The tear samples of 33 untreated nAMD patients (16 females and 17 males

70.53±10.56 years old) and another 30 healthy volunteers (16 females and 14 males

66.64±8.46 years old

with no ocular fundus diseases) were harvested in our hospital from July 2017 to March 2018. Tandem mass tag (TMT) was performed to identify the DEPs

and the obtained proteins were further analyzed using bioinformatics to screen the candidate proteins with potential prospects. In addition

parallel reaction monitoring (PRM) was used to verify the expression of selected biomarkers in the tear samples of nAMD patients.

Results

A total of 3 473 proteins were identified and quantified from the tear samples of the two groups

including 382 DEPs

with 122 of them up-regulated and 260 down-regulated (

0.05). Gene Ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that these DEPs were mainly involved in the regulation of immune response and oxidative stress. Among these proteins

monoamine oxidase A (MAOA)

transaldolase (TAL) and lysosome associated membrane protein (LAMP-2) were selected as potential biomarkers

whose expressions were then successfully verified by PRM

indicating consistency with the quantitative proteomic results of TMT.

Conclusion

TMT relative quantification and PRM targeting verification effectively screen out 382 differential proteins from tear samples between nAMD patients and healthy population

and provide 3 potential biomarkers for nAMD diagnosis.

关键词

Keywords

references

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