β-石竹烯作用于Notch1/NF-κB信号轴对脑缺血再灌注损伤大鼠的改善作用

刘京东; 陈莎; 王钰淳; 刘胜伟; 饶江燕; 王倩; 邓玲; 王萱; 董志

doi:10.16016/j.1000-5404.202009067

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β-石竹烯作用于Notch1/NF-κB信号轴对脑缺血再灌注损伤大鼠的改善作用

神经科学 | 更新时间：2026-01-09

- β-石竹烯作用于Notch1/NF-κB信号轴对脑缺血再灌注损伤大鼠的改善作用
- β-caryophyllene improves cerebral ischemia reperfusion injury in rats via Notch1/NF-κB signal axis
- 第三军医大学学报 2021年43卷第3期页码：218-225
- 作者机构：
  
  400016 重庆，重庆医科大学药学院，重庆市生物化学与分子药理重点实验室
- 作者简介：
  
  [ 董志，E-mail: 100798@cqmu.edu.cn DONG Zhi, E-mail:100798@cqmu.edu.cn
- 基金信息：
  
  重庆市基础研究与前沿探索项目(CSTC2018jcyjAX0378)
- DOI：10.16016/j.1000-5404.202009067
  中图分类号： R743.31;R914.4;R966
- 收稿：2020-09-28，
  
  修回：2020-10-27，
  
  纸质出版：2021-02-15
- 稿件说明：
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刘京东, 陈莎, 王钰淳, 等. β-石竹烯作用于Notch1/NF-κB信号轴对脑缺血再灌注损伤大鼠的改善作用[J]. 第三军医大学学报, 2021,43(3):218-225.

Jingdong LIU, Sha CHEN, Yuchun WANG, et al. β-caryophyllene improves cerebral ischemia reperfusion injury in rats via Notch1/NF-κB signal axis[J]. Journal of Third Military Medical University, 2021, 43(3): 218-225.
刘京东, 陈莎, 王钰淳, 等. β-石竹烯作用于Notch1/NF-κB信号轴对脑缺血再灌注损伤大鼠的改善作用[J]. 第三军医大学学报, 2021,43(3):218-225. DOI： 10.16016/j.1000-5404.202009067.

Jingdong LIU, Sha CHEN, Yuchun WANG, et al. β-caryophyllene improves cerebral ischemia reperfusion injury in rats via Notch1/NF-κB signal axis[J]. Journal of Third Military Medical University, 2021, 43(3): 218-225. DOI： 10.16016/j.1000-5404.202009067.

摘要

目的

探究β-石竹烯(β-caryophyllene，BCP)对脑缺血再灌注(cerebral ischemia-reperfusion，CIR)损伤大鼠的保护作用及相关机制。

方法

将SD大鼠按随机数字表法分为假手术组，缺血再灌注组，β-石竹烯低、中、高剂量组(分别为102、204、408 mg·kg

-1

)，Jagged1+BCP对照组，Jagged1(Notch1激动剂)组。模型组、β-石竹烯低、中、高剂量组及Jagged1+BCP对照组、Jagged1组使用线栓法构建SD大鼠CIR模型，缺血1.5 h，再灌注48 h后，进行脑缺血梗死体积测定以及神经行为学评分，用Western blot检测脑组织中Notch1、NF-κB p65、NF-κB p-p65及Hes1蛋白表达；HE染色观察缺血侧海马中的细胞死亡数量；ELISA法检测大鼠缺血侧海马中TNF-α及IL1-β的含量变化。

结果

与模型组进行比较，BCP给药组可明显改善大鼠神经功能缺失，减少缺血侧脑梗死体积，降低海马中Notch1、NF-κB及Hes1的蛋白表达量(

0.05)，减少TNF-α、IL-1β的释放(

0.05)，减少海马神经元细胞的死亡数。与Jagged1干预组比较，BCP给药组Notch1的激动剂Jagged1可以拮抗BCP的治疗效果。

结论

BCP能够减轻大鼠的脑缺血再灌注损伤，可能通过抑制Notch1/NF-κB减少炎性因子的释放，发挥其缺血性保护作用。

Abstract

Objective

To explore the protective effect of β-caryophyllene (BCP) on cerebral ischemia-reperfusion (I/R) rats and related mechanism.

Methods

SD rats were randomized into sham operation group

I/R group

and low-

medium- and high-dose BCP groups (102

204 and 408 mg·kg

-1

)

Jagged1 (Notch1 agonist

50 mg·kg

-1

in 3 h before I/R injury)+BCP group

and Jagged1 group. The treatments were given to the corresponding rats through intragastric infusion. In 7 d later

SD rat CIR model was established with suture-occlusion

with ischemia for 1.5 h followed by reperfusion for 48 h. The infarct volume and neurobehavioral s

core were measured; Western blotting was used to detect the cerebral protein levels of Notch1

NF-κB p65

NF-κB p-p65 and Hes1; HE staining was applied to observe the number of dead cells

and ELISA was employed to detect the changes of TNF-α and IL1-β contents in the ischemic hippocampus.

Results

BCP treatment could significantly improve the neurological deficit

reduce the infarct volume

down-regulate the protein levels of Notch1

NF-κB and Hes1 in the hippocampus (

0.05)

reduce the releases of TNF-α and IL-1β (

0.05)

and attenuate the death of hippocampal neuronal cells in the rats after cerebral I/R injury. Comparing the simple Jagged1 intervention group

Notch1 agonist

Jagged1

could antagonize the therapeutic effects of BCP.

Conclusion

BCP can attenuate cerebral I/R injury in rats

and exerts its protective effect probably through inhibiting Notch1/NF-κB signal axis to reduce the release of inflammatory factors.

关键词

Keywords

references

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