LncRNA GASL1通过Wnt/β-catenin信号通路促进血管平滑肌细胞增殖

刘彦廷; 罗然; 孙拯; 田春雷; 王雄伟

doi:10.16016/j.1000-5404.202009065

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LncRNA GASL1通过Wnt/β-catenin信号通路促进血管平滑肌细胞增殖

基础医学 | 更新时间：2026-01-09

- LncRNA GASL1通过Wnt/β-catenin信号通路促进血管平滑肌细胞增殖
- Long non-coding RNA GASL1 promotes proliferation of vascular smooth muscle cells through Wnt/β-catenin signaling pathway
- 第三军医大学学报 2021年43卷第5期页码：411-417
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81671155);湖北省卫生健康委员会面上项目(WJ2019M063);宜昌市医疗卫生科研项目基金(A18-301-03)
- DOI：10.16016/j.1000-5404.202009065
  中图分类号：
- 收稿：2020-10-10，
  
  纸质出版：2021-03-15
- 稿件说明：
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刘彦廷, 罗然, 孙拯, 等. LncRNA GASL1通过Wnt/β-catenin信号通路促进血管平滑肌细胞增殖[J]. 第三军医大学学报, 2021,43(5):411-417.

Yanting LIU, Ran LUO, Zheng SUN, et al. Long non-coding RNA GASL1 promotes proliferation of vascular smooth muscle cells through Wnt/β-catenin signaling pathway[J]. Journal of Third Military Medical University, 2021, 43(5): 411-417.
刘彦廷, 罗然, 孙拯, 等. LncRNA GASL1通过Wnt/β-catenin信号通路促进血管平滑肌细胞增殖[J]. 第三军医大学学报, 2021,43(5):411-417. DOI： 10.16016/j.1000-5404.202009065.

Yanting LIU, Ran LUO, Zheng SUN, et al. Long non-coding RNA GASL1 promotes proliferation of vascular smooth muscle cells through Wnt/β-catenin signaling pathway[J]. Journal of Third Military Medical University, 2021, 43(5): 411-417. DOI： 10.16016/j.1000-5404.202009065.

摘要

Abstract

Objective

To investigate whether long non-coding (lnc) RNA GASL1 can promote the proliferation of vascular smooth muscle cells (VSMCs) by activating the Wnt/β-catenin signaling pathway.

Methods

The mRNAs co-expression network related to lncRNA GASL1 was constructed and analyzed with Pathway analysis to predict the signaling pathway of the highest correlation with lncRNA GasL1. After LncRNA GASL1 and lncRNA negative control (lncRNA NC) were transfected into VSMCs respectively

cell counting kit-8 (CCK-8) assay was performed to detect VSMCs viability. Immunofluorescence assay was performed to determine the expression level of proliferation-related protein Bcl-2

and Western blotting to detect the levels of the proteins of Wnt/β-catenin signaling pathway. Finally

Wnt/β-catenin pathway inhibitor ICG001 was added into VSMCs along with lncRNA GASL1 for co-culturing (lncRNA GASL1+ ICG001 group)

and the alterations of VSMCs viability and bcl-2 protein level were detected and compared with those in the lncRNA GASL1+ control group.

Results

There were 374 mRNAs associated with lncRNA GASL1

of which the Wnt/β-catenin pathway had the highest correlation. CCK-8 assay showed that the VSMCs viability was higher in the lncRNA GASL1 group than in the lncRNA NC group (

0.05). The results of immunofluorescence assay and Western blotting revealed that both the Bcl-2 protein level and the levels of Wnt1 and β-catenin were increased in lncRNA GASL1 group (

0.05) when compared to the lncRNA-NC group. After co-culturing with lncRNA GASL1 and ICG001

the VSMCs viability was significantly decreased (

0.05)

and the Bcl-2 protein level was decreased when compared with the the lncRNA GASL1+control group (

0.05).

Conclusion

LncRNA GASL1 promotes the proliferation of VSMCs through the Wnt/β-catenin signaling pathway. Blocking the signaling pathway can reduce the promotive effect of lncRNA GASL1 on VSMCs.

关键词

Keywords

references

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