0.22太赫兹电磁辐射暴露致神经母细胞瘤Neuro-2a细胞损伤的非热效应研究

马秦龙; 陈纯海; 林敏; 陶嘉雯; 邓平; 高鹏; 卢永辉; 皮会丰; 何旻蒂; 张蕾; 张彦文; 余争平

doi:10.16016/j.1000-5404.202008145

您当前的位置：

首页 >

文章列表页 >

0.22太赫兹电磁辐射暴露致神经母细胞瘤Neuro-2a细胞损伤的非热效应研究

专题报道：太赫兹辐射生物效应研究 | 更新时间：2026-01-09

- 0.22太赫兹电磁辐射暴露致神经母细胞瘤Neuro-2a细胞损伤的非热效应研究
- Non-thermal effects of 0.22 terahertz electromagnetic radiation exposure-induced injury in Neuro-2a cells
- 第三军医大学学报 2020年43卷第23期页码：2267-2273, 2289
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)军事预防医学系军队劳动卫生学教研室，电磁辐射医学防护教育部重点实验室
- 作者简介：
  
  [余争平，E-mail:yuzping_tmmu@126.com YU Zhengping, E-mail:yuzping_tmmu@126.com
- 基金信息：
  
  国家重点研发计划(2017YFC0108601);国家自然科学基金面上项目(31870842)
- DOI：10.16016/j.1000-5404.202008145
  中图分类号： R-334;R146;R730.264
- 收稿：2020-08-18，
  
  修回：2020-09-25，
  
  纸质出版：2020-12-15
- 稿件说明：
移动端阅览
马秦龙, 陈纯海, 林敏, 等. 0.22太赫兹电磁辐射暴露致神经母细胞瘤Neuro-2a细胞损伤的非热效应研究[J]. 第三军医大学学报, 2020,43(23):2267-2273, 2289.

Qinlong MA, Chunhai CHEN, Min LIN, et al. Non-thermal effects of 0.22 terahertz electromagnetic radiation exposure-induced injury in Neuro-2a cells[J]. Journal of Third Military Medical University, 2020, 43(23): 2267-2273, 2289.
马秦龙, 陈纯海, 林敏, 等. 0.22太赫兹电磁辐射暴露致神经母细胞瘤Neuro-2a细胞损伤的非热效应研究[J]. 第三军医大学学报, 2020,43(23):2267-2273, 2289. DOI： 10.16016/j.1000-5404.202008145.

Qinlong MA, Chunhai CHEN, Min LIN, et al. Non-thermal effects of 0.22 terahertz electromagnetic radiation exposure-induced injury in Neuro-2a cells[J]. Journal of Third Military Medical University, 2020, 43(23): 2267-2273, 2289. DOI： 10.16016/j.1000-5404.202008145.

摘要

目的

探讨0.22太赫兹(terahertz，THz)电磁辐射暴露致小鼠神经母细胞瘤细胞株——Neuro-2a细胞损伤的非热效应特点。

方法

以Neuro-2a细胞作为体外实验暴露模型，采用频率为0.22 THz，平均功率密度为0(Sham组)、25(25 mW/cm

辐照组)、50 mW/cm

(50 mW/cm

辐照组)辐

照细胞，辐照时间为5 min，监测辐照引起的细胞培养基温度变化；于暴露后24 h检测细胞增殖(EdU阳性细胞比例和细胞活力)、凋亡(γH2AX、TUNEL阳性细胞比例、

Bax

与

Bcl2

基因表达水平)；于暴露后48 h分析细胞突触生长(突触长度、分支数目及

Tubb3

与

Syp

基因表达水平)的变化情况。

结果

与Sham组比较，两辐照组细胞培养基温度升高在0.1~0.4 ℃；EdU阳性细胞比例、细胞活力、γH2AX和TUNEL阳性细胞比例及

Bax

、

Bcl2

基因表达水平均无明显改变；仅50 mW/cm

辐照组细胞突触长度和分支数目较Sham组明显减少(

0.01)，

Tubb3

和

Syp

基因表达也明显降低(

0.05)。

结论

THz辐射暴露以非热效应为主，可抑制Neuro-2a细胞突触生长，而不影响细胞增殖和凋亡，提示神经细胞突触生长是THz辐射产生神经生物效应的敏感靶标。

Abstract

Objective

To explore the features of non-thermal effects of cell injury induced by terahertz (THz) radiation at a frequency of 0.22 THz

in a mouse neuroblastoma cell line

Neuro-2a.

Methods

The cultured Neuro-2a cells were exposed to 0.22 THz irradiation at average power densities of 0 (sham group)

and 25 and 50 mW/cm

(radiation groups)

for 5 min

respectively. The temperature changes of culture medium were monitored. The cell proliferation and apoptosis and the mRNA expression of

Bax

and

Bcl

2 at 24 h after THz exposure were detected by measuring the ratio of EdU

cells

CCK-8 assay for cell viability

immunofluorescence assay for γH2AX

cells

TUNEL assay

and real-time PCR. The neurite length

number of branch points

and mRNA expression of

Tubb

3 and

Syp

were analyzed in the Neuro-2a cells at 48 h after THz exposure.

Results

Compared with the sham group

the temperature of culture medium in the radiation groups was increased by a range from 0.1 to 0.4 ℃. There were no significant differences in the cell viability

ratios of positive cells for EdU

γH2AX and TUNEL

or the mRNA expression levels of

Bax

and

Bcl

2. The neurite length and number of branch points were obviously reduced (

0.01)

and the mRNA levels of

Tubb

3 and

Syp

were also notably down-regulated (

0.05) in the 50 mW/cm

group.

Conclusion

THz radiation mainly exhibits non-thermal effects

and can inhibit neurite outgrowth but not affect the proliferation and apoptosis in Neuro-2a cells. Our findings suggest that neurite outgrowth is a sensitive target of neurobiological effects after THz radiation exposure.

关键词

Keywords

references

CROWE T W, GLOBUS T, WOOLARD D L, et al . Terahertz sources and detectors and their application to biological sensing[J ] . Philos Trans Royal Soc Lond Ser A: Math Phys Eng Sci, 2004, 362(1815): 365-377. DOI:10.1098/rsta.2003.1327.

谢莎, 李浩然, 李玲香, 等.太赫兹通信技术综述[J].通信学报, 2020, 41(5): 168-186. DOI:10.11959/j.issn.1000- 436x.2020107.

XIE S, LI H R, LI L X, et al . Survey of terahertz communication technology[J ] . J Commun, 2020, 41(5): 168-186. DOI:10.11959/j.issn.1000-436x.2020107.

陈小霞, 李玉钊, 安建波, 等.太赫兹近场成像雷达系统误差补偿方法[J].太赫兹科学与电子信息学报, 2019, 17(1): 1-7. DOI:10.11805/TKYDA201901.0001.

CHEN X X, LI Y Z, AN J B, et al . Error compensation methods of terahertz near-field imaging radar system[J ] . J Terahertz Sci Electron Inf Technol, 2019, 17(1): 1-7. DOI:10.11805/TKYDA201901.0001.

SUN Q S, HE Y Z, LIU K, et al . Recent advances in terahertz technology for biomedical applicatio ns[J ] . Quant Imaging Med Surg, 2017, 7(3): 345-355. DOI:10.21037/qims.2017.06.02.

STERCZEWSKI L A, NOWAK K, SZLACHETKO B, et al . Chemometric evaluation of THz spectral similarity for the selection of early drug candidates[J ] . Sci Rep, 2017, 7(1): 14583. DOI:10.1038/s41598-017-14819-6.

LEE D K, KANG J H, KWON J, et al . Nano metamaterials for ultrasensitive terahertz biosensing[J ] . Sci Rep, 2017, 7(1): 8146. DOI:10.1038/s41598-017-08508-7.

DORADLA P, JOSEPH C, GILES R H. Terahertz endoscopic imaging for colorectal cancer detection: Current status and future perspectives[J]. World J Gastrointest Endosc, 2017, 9(8): 346-358. DOI:10.4253/wjge.v9.i8.346.

PLUSQUELLIC D F, SIEGRIST K, HEILWEIL E J, et al . Applications of terahertz spectroscopy in biosystems[J ] . ChemPhysChem, 2007, 8(17): 2412-2431. DOI:10.1002/cphc.200700332.

SUN Y W. A promising diagnostic method: terahertz pulsed imaging and spectroscopy[J]. World J Radiol, 2011, 3(3): 55. DOI:10.4329/wjr.v3.i3.55.

YU C, FAN S, SUN Y, et al . The potential of terahertz imaging for cancer diagnosis: a review of investigations to date[J ] . Quant Imaging Med Surg, 2012, 2(1): 33-45. DOI:10.3978/j.issn.2223-4292.2012.01.04.

YANG X, ZHAO X, YANG K, et al . Biomedical applications of terahertz spectroscopy and imaging[J ] . Trends Biotechnol, 2016, 34(10): 810-824. DOI:10.1016/j.tibtech.2016.04.008.

ROMANENKO S, BEGLEY R, HARVEY A R, et al . The interaction between electromagnetic fields at megahertz, gigahertz and terahertz frequencies with cells, tissues and organisms: risks and potential[J ] . JR Soc Interface, 2017, 14(137): 20170585. DOI:10.1098/rsif.2017.0585.

BONDAR N P, KOVALENKO I L, AVGUSTINOVICH D F, et al . Behavioral effect of terahertz waves in male mice[J ] . Bull Exp Biol Med, 2008, 145(4): 401-405. DOI:10.1007/s10517-008-0102-x.

KIRICHUK V F, ANTIPOVA O N, KRYLOVA Y A. Effect of continuous irradiation with terahertz electromagnetic waves of the NO frequency range on behavioral reactions of male albino rats under stress conditions[J]. Bull Exp Biol Med, 2014, 157(2): 184-189. DOI:10.1007/s10517-014-2521-1.

PI H F, LI M, TIAN L, et al .Enhancing lysosomal biogenesis and autophagic flux by activating the transcription factor EB protects against cadmium-induced neurotoxicity[J ] . Sci Rep, 2017, 7: 43466. DOI: 10.1038/srep43466.

CHEN C H, MA Q L, DENG P, et al . Critical role of TRPC1 in thyroid hormone-dependent dopaminergic neuron development[J ] . Biochim Biophys Acta Mol Cell Res, 2017, 1864(10): 1900-1912. DOI:10.1016/j.bbamcr.2017.07.019.

LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2 -ΔΔCT method[J ] . Methods, 2001, 25(4): 402-408. DOI:10.1006/meth.2001.1262.

CUCCURAZZU B, LEONE L, PODDA M V, et al . Exposure to extremely low-frequency (50 Hz) electromagnetic fields enhances adult hippocampal neurogenesis in C57BL/6 mice[J ] . Exp Neurol, 2010, 226(1): 173-182. DOI:10.1016/j.expneurol.2010.08.022.

MA Q L, CHEN C H, DENG P, et al . Extremely low-frequency electromagnetic fields promote in vitro neuronal differentiation and neurite outgrowth of embryonic neural stem cells via up-regulating TRPC1[J ] . PLoS ONE, 201 6, 11(3): e0150923. DOI:10.1371/journal.pone.0150923..

WANG H, PENG R Y, ZHAO L, et al . The relationship between NMDA receptors and microwave-induced learning and memory impairment: a long-term observation on Wistar rats[J ] . Int J Radiat Biol, 2015, 91(3): 262-269. DOI:10.3109/09553002.2014.988893.

CHEN C H, MA Q L, LIU C, et al . Exposure to 1800 MHz radiofrequency radiation impairs neurite outgrowth of embryonic neural stem cells[J ] . Sci Rep, 2014, 4: 5103. DOI:10.1038/srep05103.

ZHI T S, CHENG T P, QING L L, et al . Exposure effects of terahertz waves on primary neurons and neuron-like cells under nonthermal conditions[J ] . Biomed Environ Sci, 2019, 32(10): 739-754.

PERERA P, APPADOO D, CHEESEMAN S, et al . PC 12 pheochromocytoma cell response to super high frequency terahertz radiation from synchrotron source[J ] . Cancers, 2019, 11(2): 162. DOI:10.3390/cancers11020162.

DUKA M V, DVORETSKAYA L N, BABELKIN N S, et al . Numerical and experimental studies of mechanisms underlying the effect of pulsed broadband terahertz radiation on nerve cells[J ] . Quantum Electron, 2014, 44(8): 707-712. DOI:10.1070/qe2014v044n08abeh015486.

TITOVA L V, AYESHESHIM A K, GOLUBOV A, et al . Intense THz pulses cause H2AX phosphorylation and activate DNA damage response in human skin tissue[J ] . Biomed Opt Express, 2013, 4(4): 559. DOI:10.1364/boe.4.000559.

BOURNE N, CLOTHIER R H, D'ARIENZO M, et al . The effects of terahertz radiation on human keratinocyte primary cultures and neural cell cultures[J ] . Altern Lab Animals, 2008, 36(6): 667-684. DOI:10.1177/0261192908036 00610.

WILMINK G J, RIVEST B D, ROTH C C, et al . In vitro investigation of the biological effects associated with human dermal fibroblasts exposed to 2.52 THz radiation[J ] . Lasers Surg Med, 2011, 43(2): 152-163. DOI:10.1002/lsm.20960..

KIM K T, PARK J, JO S J, et al . High-power femtosecond-terahertz pulse induces a wound response in mouse skin[J ] . Sci Rep, 2013, 3: 2296. DOI:10.1038/srep02296.

BOGOMAZOVA A N, VASSINA E M, GORYACHKOVSKAYA T N, et al . No DNA damage response and negligible genome-wide transcription al changes in human embryonic stem cells exposed to terahertz radiation[J ] . Sci Rep, 2015, 5: 7749. DOI:10.1038/srep07749.

ALEXANDROV B S, PHIPPS M L, ALEXANDROV L B, et al . Specificity and heterogeneity of terahertz radiation effect on gene expression in mouse mesenchymal stem cells[J ] . Sci Rep, 2013, 3: 1184. DOI:10.1038/srep01184.

FRANCHINI V, DE SANCTIS S, MARINACCIO J, et al . Study of the effects of 0.15 terahertz radiation on genome integrity of adult fibroblasts[J ] . Environ Mol Mutagen, 2018, 59(6): 476-487. DOI:10.1002/em.22192.

DE AMICIS A, DE SANCTIS S, CRISTOFARO S D, et al . Biological effects of in vitro THz radiation exposure in human foetal fibroblasts[J ] . Mutat Res-Gen Toxi En, 2015, 793: 150-160. DOI:10.1016/j.mrgentox.2015.06.003..

ALEXANDROV B S, GELEV V, BISHOP A R, et al . DNA breathing dynamics in the presence of a terahertz field[J ] . Phys Lett A, 2010, 374(10): 1214-1217. DOI:10.1016/j.physleta.2009.12.077.

HOMENKO A, KAPILEVICH B, KORNSTEIN R, et al . Effects of 100 GHz radiation on alkaline phosp hatase activity and antigen-antibody interaction[J ] . Bioelectromagnetics, 2009, 30(3): 167-175. DOI:10.1002/bem.20466.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

静息期精原前体细胞Rb1基因敲除致精原干细胞特化异常机制初探

生物钟基因PER1调控口腔鳞癌细胞中BAX、BCL2和PCNA表达的昼夜节律

靶向抑制DNAJC1基因对多发性骨髓瘤细胞凋亡和增殖的影响