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NLRP3炎症小体激活在太赫兹暴露所致小鼠皮肤炎症中的作用
NLRP3 inflammasome activation mediates terahertz exposure-induced skin aseptic inflammation in mice
- 第三军医大学学报 2020年43卷第23期 页码：2290-2295
- 作者机构：
400038 重庆，陆军军医大学(第三军医大学)军事预防医学系军队劳动卫生学教研室，电磁辐射医学防护教育部重点实验室
- 作者简介：
[余争平，E-mail:yuzping_tmmu@126.com YU Zhengping, E-mail:yuzping_tmmu@126.com
- 基金信息：国家重点研发计划(2017YFC0108601);国家自然科学基金面上项目(31870842)
DOI：10.16016/j.1000-5404.202008142
中图分类号： R146;R363.21;R758.13收稿：2020-08-18，
修回：2020-09-28，
纸质出版：2020-12-15
- 稿件说明：
移动端阅览
高鹏, 卢永辉, 马秦龙, 等. NLRP3炎症小体激活在太赫兹暴露所致小鼠皮肤炎症中的作用[J]. 第三军医大学学报, 2020,43(23):2290-2295.
Peng GAO, Yonghui LU, Qinlong MA, et al. NLRP3 inflammasome activation mediates terahertz exposure-induced skin aseptic inflammation in mice[J]. Journal of Third Military Medical University, 2020, 43(23): 2290-2295.
高鹏, 卢永辉, 马秦龙, 等. NLRP3炎症小体激活在太赫兹暴露所致小鼠皮肤炎症中的作用[J]. 第三军医大学学报, 2020,43(23):2290-2295. DOI： 10.16016/j.1000-5404.202008142.
Peng GAO, Yonghui LU, Qinlong MA, et al. NLRP3 inflammasome activation mediates terahertz exposure-induced skin aseptic inflammation in mice[J]. Journal of Third Military Medical University, 2020, 43(23): 2290-2295. DOI： 10.16016/j.1000-5404.202008142.
摘要
目的
2
研究太赫兹(terahertz，THz)辐射暴露对小鼠皮肤组织可能的损伤影响，探讨太赫兹暴露致皮肤无菌性炎症的潜在机制。
方法
2
采用C57BL/6J小鼠为实验动物模型，使用频率为0.22 THz的太赫兹辐射局部暴露小鼠皮肤组织，太赫兹峰值功率密度为25、80、160 mW/cm
2
，占空比1:5，暴露时间5 min和10 min。暴露后观察动物皮肤炎症因子释放、氧化应激反应和NLRP3炎症小体激活。并通过Caspase-1抑制剂，探讨NLRP3炎症小体激活在太赫兹辐射致皮肤炎症中的作用。
结果
2
160 mW/cm
2
太赫兹辐照10 min可导致小鼠皮肤氧化应激反应，并引起皮肤组织内IL-1β、IL-6和PGE2含量升高(
P
<
0.05)。80 mW/cm
2
和160 mW/cm
2
暴露10 min均可导致皮肤组织NLRP3炎症小体激活，而Caspase-1抑制剂则可显著降低皮肤组织炎症因子水平(
P
<
0.05)。
结论
2
太赫兹电磁辐射暴露能够引起小鼠皮肤氧化应激反应和无菌性炎症，NLRP3炎症小体激活则介导了太赫兹暴露所致的皮肤组织炎症，并可能与氧化应激有关。
Abstract
Objective
2
To investigate the biological effects of terahertz (THz) exposure on the skin injury of mice
and explore the mechanism of the induced skin aseptic inflammation.
Methods
2
The hair-removed skin in the back of C57BL/6J mice were exposed to 0.22 THz irradiation
at a peak power density of 25
80 and 160 mW/cm
2
respectively and a duty factor of 1: 5
for 5 or 10 min. The release of inflammatory factors
oxidative stress and activation of NLR P3 inflammasome were detected after the exposure. Caspase-1 specific inhibitor Z-YVAD was employed to determine the role of the activation in the inflammation.
Results
2
Exposure to 160 mW/cm
2
THz irradiation for 10 min induced oxidative stress
and the releases of PGE2
IL-1β and IL-6 in the skin (
P
<
0.05). And the 80 mW/cm
2
and 160 mW/cm
2
THz exposure activated the NLRP3 inflammasome in the skin
and the Caspase-1 inhibitor reduced the inflammatory factors induced by the THz irradiation (
P
<
0.05).
Conclusion
2
THz exposure induces oxidative stress and aseptic inflammation. Activation of NLRP3 inflammasome mediates the skin aseptic inflammation induced by THz irradiation
which might be associated with oxidative stress.
关键词
Keywords
references
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