浏览全部资源
扫码关注微信
太赫兹辐射暴露致小鼠皮肤损伤效应研究
Effects of terahertz exposure on skin injury in mouse model
- 第三军医大学学报 2020年43卷第23期 页码：2282-2289
- 作者机构：
400038 重庆，陆军军医大学(第三军医大学)军事预防医学系军队劳动卫生学教研室，电磁辐射医学防护教育部重点实验室
- 作者简介：
[余争平，E-mail:yuzping_tmmu@126.com YU Zhengping, E-mail:yuzping_tmmu@126.com
- 基金信息：国家重点研发计划(2017YFC0108601);国家自然科学基金面上项目(31870842)
DOI：10.16016/j.1000-5404.202008138
中图分类号： R-334;R146;R758.13收稿：2020-08-18，
修回：2020-09-27，
纸质出版：2020-12-15
- 稿件说明：
移动端阅览
陈纯海, 马秦龙, 陶嘉雯, 等. 太赫兹辐射暴露致小鼠皮肤损伤效应研究[J]. 第三军医大学学报, 2020,43(23):2282-2289.
Chunhai CHEN, Qinlong MA, Jiawen TAO, et al. Effects of terahertz exposure on skin injury in mouse model[J]. Journal of Third Military Medical University, 2020, 43(23): 2282-2289.
陈纯海, 马秦龙, 陶嘉雯, 等. 太赫兹辐射暴露致小鼠皮肤损伤效应研究[J]. 第三军医大学学报, 2020,43(23):2282-2289. DOI： 10.16016/j.1000-5404.202008138.
Chunhai CHEN, Qinlong MA, Jiawen TAO, et al. Effects of terahertz exposure on skin injury in mouse model[J]. Journal of Third Military Medical University, 2020, 43(23): 2282-2289. DOI： 10.16016/j.1000-5404.202008138.
摘要
目的
2
探讨太赫兹(terahertz，THz)辐射暴露所致皮肤组织损伤的生物效应特点及其潜在的分子机制。
方法
2
采用C57BL/6J小鼠为实验动物模型，通过频率为0.22 THz，平均功率密度为0、25、50 mW/cm
2
的THz辐照动物，辐照时间为5 min，观察辐照引起的暴露部位皮肤升温曲线特点和皮肤组织病理损伤特征，同时通过对辐照部位皮肤组织进行转录组测序及生物信息学分析，揭示损伤的整体效应及潜在分子机制，并进一步通过ELISA验证RNA-seq揭示的相关效应。
结果
2
25 mW/cm
2
THz辐照不引起明显的升温效应，但50 mW/cm
2
THz辐照能够导致辐照动物皮肤平均温度迅速升高超过2 ℃
并且导致辐照部位皮肤组织出现上皮脱落及出血等病理损伤特征。通过对损伤皮肤组织进行转录组测序，共筛选出上调基因49个，下调基因27个。对这些基因进行GO及KEGG分析结果显示，其主要功能为调控机体组织免疫及炎症反应等。ELISA检测结果显示，THz暴露后皮肤炎症因子IL-1β、IL-6及TNF-α表达明显升高(
P
<
0.05
P
<
0.01)。
结论
2
THz辐射导致暴露部位皮肤出现明显的热效应损伤及引起炎症反应等损伤效应，并导致一系列基因表达异常。
Abstract
Objective
2
To explore the characteristics of biological effects of terahertz (THz) exposure on the skin injury
and investigate the underlying mechanisms.
Methods
2
The hair-removed skin of C57BL/6J mice were exposed to 0.22 THz irradiation
at an average power density of 0
25 and 50 mW/cm
2
respectively
for 5 min. The temperature curve and pathological changes in the exposed area were observed and analyzed. The gene expression in the skin area was detected using RNA-seq analysis and bioinformatics analysis for global changes of the impairment and underlying molecular mechanisms. Finally
ELISA was used to verify inflammatory responses in the skin revealed by RNA-seq.
Results
2
Though 25 mW/cm
2
THz irradiation did not induce obvious temperature change
50 mW/cm
2
THz irradiation exposure significantly raised the temperature higher than 2 ℃
and caused epithelial detachment and bleeding in the exposed area. RNA-seq analysis found 49 genes up-regulated and 27 genes down-regulated in the injured skin area. The following Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that these genes were majorly involved in immune responses and inflammatory responses. The results of ELISA indicated that the expression levels of IL-1β
IL-6
and TNF-α were increased remarkably after exposure.
Conclusion
2
THz irradiation exposure causes significant thermal effects and other injuries
including inflammatory responses
and induces abnormal expression of a series of genes in the exposed skin area.
关键词
Keywords
references
CROWE T W, GLOBUS T, WOOLARD D L, et al . Terahertz sources and detectors and their application to biological sensing[J ] . Philos Trans A Math Phys Eng Sci, 2004, 362(1815): 365-377. DOI:10.1098/rsta. 2003.1327.
赵国忠, 申彦春, 刘影.太赫兹技术在军事和安全领域的应用[J].电子测量与仪器学报, 2015, 29(8): 1097-1101. DOI:10.13382/j.jemi.2015.08.001.
ZHAO G Z, SHEN Y C, LIU Y. Application of terahertz technology in military and security field[J]. J Electron Meas Instrum, 2015, 29(8): 1097-1101. DOI:10.13382/j.jemi.2015. 08.001.
PLUSQUELLIC D F, SIEGRIST K, HEILWEIL E J, et al . Applications of terahertz spectroscopy in biosystems[J ] . ChemPhysChem, 2007, 8(17): 2412-2431. DOI:10.1002/cphc. 200700332.
孙金海.太赫兹电磁波在生物医学领域中的应用原理及进展[J].首都师范大学学报(自然科学版), 2004, 25(4): 17-22. DOI:10.3969/j.issn.1004-9398.2004.04.006.
SUN J H. Applications and developments of terahertzelectromagnetic wave in biological and medical fields[J]. J Cap Norm Univ Nat Sci Ed, 2004, 25(4): 17-22. DOI:10.3969/j.issn.1004-9398.2004.04.006.
SUN Q, HE Y, LIU K, et al . Recent advances in terahertz technology for biomedical applications[J ] . Quant Imaging Med Surg, 2017, 7(3): 345-355. DOI:10.21037/qims. 2017.06.02.
ROMANENKO S, BEGLEY R, HARVEY A R, et al . The interaction between electromagnetic fields at megahertz, gigahertz and terahertz frequencies with cells, tissues and organisms: risks and potential[J ] . J Royal Soc Interface, 2017, 14(137): 20170585. DOI:10.1098/rsif.2017.0585.
PARKER J E, BEASON C W, STURGEON S P, et al . Revisiting 35 and 94 GHz millimeter wave exposure to the non-human primate eye[J ] . Heal Phys, 2020, 119(2): 206 -215. DOI:10.1097/hp.0000000000001216.
TITOVA L V, AYESHESHIM A K, GOLUBOV A, et al . Intense THz pulses cause H2AX phosphorylation and activate DNA damage response in human skin tissue[J ] . Biomed Opt Express, 2013, 4(4): 559-568. DOI:10.1364/boe.4.000559.
WILMINK G J, RIVEST B D, ROTH C C, et al . In vitro investigation of the biological effects associated with human dermal fibroblasts exposed to 2.52 THz radiation[J ] . Lasers Surg Med, 2011, 43(2): 152-163. DOI:10.1002/lsm.20960..
BONDAR N P, KOVALENKO I L, AVGUSTINOVICH D F, et al . Behavioral effect of terahertz waves in male mice[J ] . Bull Exp Biol Med, 2008, 145(4): 401-405. DOI:10.1007/s10517-008-0102-x.
KIRICHUK V F, ANTIPOVA O N, KRYLOVA Y A. Effect of continuous irradiation with terahertz electromagnetic waves of the NO frequency range on behavioral reactions of male albino rats under stress conditions[J]. Bull Exp Biol Med, 2014, 157(2): 184-189. DOI:10.1007/s10517-014- 2521-1.
ALEXANDROV B S, PHIPPS M L, ALEXANDROV L B, et al . Specificity and heterogeneity of terahertz radiation e ffect on gene expression in mouse mesenchymal stem cells[J ] . Sci Rep, 2013, 3: 1184. DOI:10.1038/srep01184.
FRANCHINI V, DE SANCTIS S, MARINACCIO J, et al . Study of the effects of 0.15 terahertz radiation on genome integrity of adult fibroblasts[J ] . Environ Mol Mutagen, 2018, 59(6): 476-487. DOI:10.1002/em.22192.
KOYAMA S, NARITA E, SHIMIZU Y, et al . Twenty four-hour exposure to a 0.12 THz electromagnetic field does not affect the genotoxicity, morphological changes, or expression of heat shock protein in HCE-T cells[J ] . Int J Environ Res Public Heal, 2016, 13(8): 793. DOI:10.3390/ijerph13080793.
BOGOMAZOVA A N, VASSINA E M, GORYACHKOV-SKAYA T N, et al . No DNA damage response and negligible genome-wide transcriptional changes in human embryonic stem cells exposed to terahertz radiation[J ] . Sci Rep, 2015, 5: 7749. DOI:10.1038/srep07749.
TITOVA L V, AYESHESHIM A K, GOLUBOV A, et al . Intense THz pulses down-regulate genes associated with skin cancer and psoriasis: a new therapeutic avenue?[J ] . Sci Rep, 2013, 3: 2363. DOI:10.1038/srep02363.
TSOY A, SAL IEV T, ABZHANOVA E, et al . The effects of mobile phone radiofrequency electromagnetic fields on β-amyloid-induced oxidative stress in human and rat primary astrocytes[J ] . Neuroscience, 2019, 408: 46-57. DOI:10.1016/j.neuroscience.2019.03.058.
JEONG Y, SON Y, HAN N K, et al . Impact of long-term RF-EMF on oxidative stress and neuroinflammation in aging brains of C57BL/6 mice[J ] . Int J Mol Sci, 2018, 19(7): 2103. DOI:10.3390/ijms19072103.
0
浏览量
0
下载量
0
CSCD
- 网络PDF下载
- Meta-XML下载
- BibTeX下载
- Endnote下载
- RefMan 下载
- NoteExpress下载
- NoteFirst下载
关联资源
相关文章
相关作者
相关机构
- 技术支持由北京北大方正电子有限公司提供 京ICP备09064830号-19
京公网安备11010802024621 - 本系统建议在Chrome、 IE9+ 以上版本浏览器阅读本站内容，360浏览器请切换至极速模式
- Cookies帮助我们提供服务并提供个性化体验。使用本网站，即表示您同意我们使用Cookies
