支气管喷入高剂量贫铀染毒犬模型早期损伤特点研究

付维林; 陈翔宇; 李蓉; 朱勇; 田铸; 曾峰; 肖瑶; 刘明华

doi:10.16016/j.1000-5404.202007210

您当前的位置：

首页 >

文章列表页 >

支气管喷入高剂量贫铀染毒犬模型早期损伤特点研究

军事医学(2020年度重庆市出版专项资金资助项目) | 更新时间：2026-01-09

- 支气管喷入高剂量贫铀染毒犬模型早期损伤特点研究
- Features of early injuries in canine model of high dose depleted uranium exposure through bronchial spraying
- 第三军医大学学报 2021年43卷第1期页码：52-58
- 作者机构：
- 作者简介：
- 基金信息：
  
  军队后勤科研重点项目(BWS15J009);军队后勤科研重点项目(BLJ20J005)
- DOI：10.16016/j.1000-5404.202007210
  中图分类号：
- 收稿：2020-07-27，
  
  纸质出版：2021-01-15
- 稿件说明：
移动端阅览
付维林, 陈翔宇, 李蓉, 等. 支气管喷入高剂量贫铀染毒犬模型早期损伤特点研究[J]. 第三军医大学学报, 2021,43(1):52-58.

Weilin FU, Xiangyu CHEN, Rong LI, et al. Features of early injuries in canine model of high dose depleted uranium exposure through bronchial spraying[J]. Journal of Third Military Medical University, 2021, 43(1): 52-58.
付维林, 陈翔宇, 李蓉, 等. 支气管喷入高剂量贫铀染毒犬模型早期损伤特点研究[J]. 第三军医大学学报, 2021,43(1):52-58. DOI： 10.16016/j.1000-5404.202007210.

Weilin FU, Xiangyu CHEN, Rong LI, et al. Features of early injuries in canine model of high dose depleted uranium exposure through bronchial spraying[J]. Journal of Third Military Medical University, 2021, 43(1): 52-58. DOI： 10.16016/j.1000-5404.202007210.

摘要

Abstract

Objective

To explore the features of early damages due to high dose depleted uranium (DU) exposure through bronchial spraying.

Methods

A canine model of acute lung injury was established by bronchial spraying of 20 mg/kg DU suspension. Serum uranium content

arterial blood gas

blood routine

liver function

kidney function

myocardial enzymes

cytokines

and CT examination were tested or performed at pre-exposure

and 3 and 8 h and 1

5 and 7 d after exposure.

Results

The serum uranium content was significantly increased at all the time points after exposure than the content before exposure (

0.05). And the content at 8 h and 1

5 and 7 d was notably higher than the content at 3 h (

0.01)

and that at 8 h and 7 d was obviously higher than it at 1

3 and 5 d (

0.05). Compared with the values before exposure

the counts of leukocytes and neutrophils were risen at 8 h and 5 d after exposure (

0.05)

the levels of creatine kinase (CK)

creatine kinase-MB (CK-MB) and aspartate aminotransferase (AST) were increased sharply at 3 h (

0.05)

the creatinine level was dramatically elevated at 5 and 7 d (

0.01)

and the level of IL-8 was raised at 3 and 5 d after DU exposure (

0.05). The CT scanning results showed that the scores of pulmonary shadows were higher at all time points after than before exposure

and there were significant differences in the score at 3 and 8 h when compared with oth

er time points (

0.01). The score at 1 d was much higher than that at 5 and 7 d (

0.05).

Conclusion

The distributing rate of high dose DU exposure through bronchial spraying is possibly in line with two-compartment model. Severe inflammation occurs in the lungs at the 8th hour after exposure. Renal function is obviously impaired at day 5

along with another rise of serum uranium content.

关键词

Keywords

references

HON Z ÖSTERREICHER J NAVRÁTIL L Depleted uranium and its effects on humans Sustainability 2015 7 4 4063 4077 10.3390/su7044063 HON Z, ÖSTERREICHER J, NAVRÁTIL L. Depleted uranium and its effects on humans[J]. Sustainability, 2015, 7(4): 4063-4077. DOI:10.3390/su7044063.

Armando F Clara G Daniela F Depleted uranium and human health Curr Med Chem 2018 25 1 49 64 10.2174/0929867324666170426102343 Armando F, Clara G, Daniela F, et al . Depleted uranium and human health[J ] . Curr Med Chem, 2018, 25(1):49-64. DOI:10.2174/092986732466617042 6102343.

BURKART W DANESI P R HENDRY J H Properties, use and health effects of depleted uranium Int Congr Ser 2005 1276 133 136 10.1016/j.ics.2004.09.047 BURKART W, DANESI P R, HENDRY J H. Properties, use and health effects of depleted uranium[J]. Int Congr Ser, 2005, 1276: 133-136. DOI:10.1016/j.ics.2004.09.047.

艾国平李蓉肖桃元大鼠长期摄入贫铀后的病理形态学特点观察解放军医学杂志 2007 32 10 1036 1039 10.3321/j.issn:0577-7402.2007.10.011 艾国平, 李蓉, 肖桃元, 等.大鼠长期摄入贫铀后的病理形态学特点观察[J].解放军医学杂志, 2007, 32(10): 1036-1039. DOI:10.3321/j.issn:0577-7402.2007.10.011.

AI G P LI R XIAO T Y Pathological changes of rats after chronic ingestion of depleted uranium Med J Chin PLA 2007 32 10 1036 1039 10.3321/j.issn:0577-7402.2007.10.011 AI G P, LI R, XIAO T Y, et al . Pathological changes of rats after chronic ingestion of depleted uranium[J ] . Med J Chin PLA, 2007, 32(10): 1036-1039. DOI:10.3321/j.issn:0577-7402.2007.10.011.

潘秀颉杨陟华曹珍山大鼠吸入贫铀气溶胶后体内铀的分布中华放射医学与防护杂志 2009 29 1 23 26 10.3760/cma.j.issn.0254-5098.2009.01.007 潘秀颉, 杨陟华, 曹珍山, 等.大鼠吸入贫铀气溶胶后体内铀的分布[J].中华放射医学与防护杂志, 2009, 29(1): 23-26. DOI:10.3760/cma.j.issn.0254-5098.2009.01.007.

PAN X J YANG Z H CAO Z S Distribution of uranium in Rata inhaled with depleted uranium aerosols Chin J Radiol Med Prot 2009 29 1 23 26 10.3760/cma.j.issn.0254-5098.2009.01.007 PAN X J, YANG Z H, CAO Z S, et al . Distribution of uranium in Rata inhaled with depleted uranium aerosols[J ] . Chin J Radiol Med Prot, 2009, 29(1): 23-26. DOI:10.3760/cma.j.issn.0254-5098.2009.01.007.

曹珍山朱茂祥杨陟华大鼠吸入贫铀气溶胶后主要器官的病理损伤特点中国辐射卫生 2005 14 2 81 84 10.3969/j.issn.1004-714X.2005.02.001 曹珍山, 朱茂祥, 杨陟华, 等.大鼠吸入贫铀气溶胶后主要器官的病理损伤特点[J].中国辐射卫生, 2005, 14(2): 81-84. DOI:10.3969/j.issn.1004-714X.2005.02.001.

CAO Z S ZHU M X YANG Z H Characteristic pathological changes of main organs of rats after inhalation of depleted uranium aerosol Chin J Radiol Heal 2005 14 2 81 84 10.3969/j.issn.1004-714X.2005.02.001 CAO Z S, ZHU M X, YANG Z H, et al . Characteristic pathological changes of main organs of rats after inhalation of depleted uranium aerosol[J ] . Chin J Radiol Heal, 2005, 14(2): 81-84. DOI:10.3 969/j.issn.1004-714X.2005.02.001.

岳永超李明华冀楠贫铀的体内外毒性及流行病学研究进展现代生物医学进展 2018 18 14 2784 2787 10.13241/j.cnki.pmb.2018.14.041 岳永超, 李明华, 冀楠, 等.贫铀的体内外毒性及流行病学研究进展[J].现代生物医学进展, 2018, 18(14): 2784-2787. DOI:10.13241/j.cnki.pmb.2018.14.041.

YUE Y C LI M H JI N Research progress of depleted uranium toxicity in vivo/vitro and epidemiology Prog Mod Biomed 2018 18 14 2784 2787 10.13241/j.cnki.pmb.2018.14.041 YUE Y C, LI M H, JI N, et al . Research progress of depleted uranium toxicity in vivo/vitro and epidemiology[J ] . Prog Mod Biomed, 2018, 18(14): 2784-2787. DOI:10.13241/j.cnki.pmb.2018.14.041.

张斌段蕴铀贺文博贫铀吸入性肺损伤犬模型的建立解放军医学杂志 2011 36 2 188 191 https://www.cnki.com.cn/Article/CJFDTOTAL-JFJY201102031.htm 张斌, 段蕴铀, 贺文博, 等.贫铀吸入性肺损伤犬模型的建立[J].解放军医学杂志, 2011, 36(2): 188-191.

ZHANG B DUAN Y Y HE W B Reproduction of a model of lung injury induced by depleted uranium inhalation in canine Med J Chin PLA 2011 36 2 188 191 https://www.cnki.com.cn/Article/CJFDTOTAL-JFJY201102031.htm ZHANG B, DUAN Y Y, HE W B, et al . Reproduction of a model of lung injury induced by depleted uranium inhalation in canine[J ] . Med J Chin PLA, 2011, 36(2): 188-191.

REN J HAO Y H GAO R Effect of a novel polyethylene glycol compound on lung lavage in dogs after the inhalation of depleted uranium dust Int J Radiat Biol 2018 94 5 462 471 10.1080/09553002.2018.1446228 REN J, HAO Y H, GAO R, et al . Effect of a novel polyethylene glycol compound on lung lavage in dogs after the inhalation of depleted uranium dust[J ] . Int J Radiat Biol, 2018, 94(5): 462-471. DOI:10.1080/09553002.2018.1446228.

张斌 . 全肺灌洗对贫铀吸入性肺损伤干预作用的实验研究 [D ] . 上海 : 第二军医大学 , 2011 . 张斌.全肺灌洗对贫铀吸入性肺损伤干预作用的实验研究[D].上海: 第二军医大学, 2011.

ZHANG B . Interventional effect of whole lung lavage on lung injury induced by depleted uranium aspiration [D ] . Shanghai : Second Military Medical University , 2011 . ZHANG B. Interventional effect of whole lung lavage on lung injury induced by depleted uranium aspiration[D]. Shanghai: Second Military Medical University, 2011.

苏翠文 . 硒对贫铀气管灌注大鼠致损伤的防护作用 [D ] . 合肥 : 安徽医科大学 , 2012 . 苏翠文.硒对贫铀气管灌注大鼠致损伤的防护作用[D].合肥: 安徽医科大学, 2012.

SU C W . Protection of selenium on dysfunction induced by depleted uranium instillation through trachea in rats [D ] . Hefei : Anhui Medical University , 2012 . SU C W. Protection of selenium on dysfunction induced by depleted uranium instillation through trachea in rats[D]. Hefei: Anhui Medical University, 2012.

SCILLIA P KAFI S A MÉLOT C Oleic acid-induced lung injury: thin-section CT evaluation in dogs Radiology 2001 219 3 724 731 10.1148/radiology.219.3.r01jn01724 SCILLIA P, KAFI S A, MÉLOT C, et al . Oleic acid-induced lung injury: thin-section CT evaluation in dogs[J ] . Radiology, 2001, 219(3): 724-731. DOI:10.1148/radiology.219.3.r01jn01724.

曹佳曹务春栗永萍程天民军事预防医学北京人民军医出版社 2014 900 914 曹佳, 曹务春, 栗永萍, 等.程天民军事预防医学[M].北京:人民军医出版社, 2014:900-914.

CAO J CAO W C SU Y P Cheng Tianmin military preventive medicine Beijing People's Military Medical Publishing House 2014 900 914 CAO J, CAO W C, SU Y P, et al . Cheng Tianmin military preventive medicine[M ] . Beijing: People's Military Medical Publishing House, 2014:900-914.

孙建宁药理学北京中国中医药出版社 2016 28 孙建宁.药理学[M].北京:中国中医药出版社, 2016: 28.

SUN J N Pharmacology Beijing China Press of Traditional Chinese Medicine 2016 28 SUN J N. Pharmacology[M]. Beijing: China Press of Traditional Chinese Medicine, 2016: 28.

刘树铮铀毒理学北京原子能出版社 1995 304 310 刘树铮.铀毒理学[M].北京:原子能出版社, 1995: 304-310.

LIU S Z Uranium toxicology Beijing Atomic Energy Press 1995 304 310 LIU S Z. Uranium toxicology[M]. Beijing:Atomic Energy Press, 1995: 304-310.

SONG Y SALBU B TEIEN H C Hepatic transcriptional responses in Atlantic salmon (Salmo salar) exposed to gamma radiation and depleted uranium singly and in combination Sci Total Environ 2016 562 270 279 10.1016/j.scitotenv.2016.03.222 SONG Y, SALBU B, TEIEN H C, et al . Hepatic transcriptional responses in Atlantic salmon (Salmo salar) exposed to gamma radiation and depleted uranium singly and in combination[J ] . Sci Total Environ, 2016, 562: 270-279. DOI:10.1016/j.scitotenv.2016.03.222.

NORDBERG G F FOWLER B A NORDBERG M Handbook on the toxicology of metals London EIsevier 2015 1307 1345 NORDBERG G F, FOWLER B A, NORDBERG M. Handbook on the toxicology of metals[M]. London: EIsevier, 2015: 1307-1345.

冷言冰李蓉艾国平贫铀长期摄入对大鼠肾脏损伤效应的研究第三军医大学学报 2008 30 18 1686 1689 https://www.cnki.com.cn/Article/CJFDTOTAL-DSDX200818003.htm 冷言冰, 李蓉, 艾国平, 等.贫铀长期摄入对大鼠肾脏损伤效应的研究[J].第三军医大学学报, 2008, 30(18): 1686-1689.

LENG Y B LI R AI G P Toxic effects of depleted uranium on rat kidney after long-term ingestion J Third Mil Med Univ 2008 30 18 1686 1689 https://www.cnki.com.cn/Article/CJFDTOTAL-DSDX200818003.htm LENG Y B, LI R, AI G P, et al . Toxic effects of depleted uranium on rat kidney after long-term ingestion[J ] . J Third Mil Med Univ, 2008, 30(18): 1686-1689.

KITAHARA K NUMAKO C TERADA Y Uranium XAFS analysis of kidney from rats exposed to uranium J Synchrotron Radiat 2017 24 Pt 2 456 462 10.1107/S1600577517001850 KITAHARA K, NUMAKO C, TERADA Y, et al . Uranium XAFS analysis of kidney from rats exposed to uranium[J ] . J Synchrotron Radiat, 2017, 24(Pt 2): 456-462. DOI:10.1107/S1600577517001850.

JIM V LAVIOLETTE C BRIEHL M M Spatial distribution of uranium in mice kidneys detected by laser ablation inductively coupled plasma mass spectrometry J Appl Bioanal 2017 3 3 43 48 10.17145/jab.17.007 JIM V, LAVIOLETTE C, BRIEHL M M, et al . Spatial distribution of uranium in mice kidneys detected by laser ablation inductively coupled plasma mass spectrometry[J ] . J Appl Bioanal, 2017, 3(3): 43-48. DOI:10.17145/jab.17.007.

邹义洲医学免疫学北京中国医药科技出版社 2018 23 29 邹义洲.医学免疫学[M].北京:中国医药科技出版社, 2018:23-29.

ZOU Y Z Medical immunology Beijing China Medical Science and Technology Press 2018 23 29 ZOU Y Z. Medical immunology[M]. Beijing: China Medical Science and Technology Press, 2018:23-29.

ORONA N S TASAT D R Uranyl nitrate-exposed rat alveolar macrophages cell death: influence of superoxide anion and TNF α mediators Toxicol Appl Pharmacol 2012 261 3 309 316 10.1016/j.taap.2012.04.022 ORONA N S, TASAT D R. Uranyl nitrate-exposed rat alveolar macrophages cell death: influence of superoxide anion and TNF α mediators[J]. Toxicol Appl Pharmacol, 2012, 261(3): 309-316. DOI:10.1016/j.taap.2012.04.022.

杨扬李威王左敏牙周炎合并AECOPD患者血清IL-6、IL-8水平及相关性研究安徽医科大学学报 2018 53 6 964 966 10.19405/j.cnki.issn1000-1492.2018.06.029 杨扬, 李威, 王左敏, 等.牙周炎合并AECOPD患者血清IL-6、IL-8水平及相关性研究[J].安徽医科大学学报, 2018, 53(6): 964-966. DOI:10.19405/j.cnki.issn1000-1492.2018.06.029.

YANG Y LI W WANG Z M Clinical significance of IL-6 and IL-8 in patients with periodontal disease and AECOPD and their correlations Acta Univ Med Anhui 2018 53 6 964 966 10.19405/j.cnki.issn1000-1492.2018.06.029 YANG Y, LI W, WANG Z M, et al . Clinical significance of IL-6 and IL-8 in patients with periodontal disease and AECOPD and their correlations[J ] . Acta Univ Med Anhui, 2018, 53(6): 964-966. DOI:10.19405/j.cnki.issn1000-1492.2018.06.029.

HORIE S MASTERSON C DEVANEY J Stem cell therapy for acute respiratory distress syndrome: a promising future? Curr Opin Crit Care 2016 22 1 14 20 10.1097/mcc.0000000000000276 HORIE S, MASTERSON C, DEVANEY J, et al . Stem cell therapy for acute respiratory distress syndrome: a promising future?[J ] . Curr Opin Crit Care, 2016, 22(1): 14-20. DOI:10.1097/mcc.0000000000000276.

伍冬冬潘频华覃庆武急性肺损伤/急性呼吸窘迫综合征发病机制研究进展中华结核和呼吸杂志 2015 38 7 524 527 10.3760/cma.j.issn.1001-0939.2015.07.013 伍冬冬, 潘频华, 覃庆武等.急性肺损伤/急性呼吸窘迫综合征发病机制研究进展[J].中华结核和呼吸杂志, 2015, 38(7):524-527. DOI:10.3760/cma.j.issn.1001-0939.2015.07.013.

WU D D PAN P H QIN Q W Advances in the pathogenesis of acute lung injury/acute respiratory distress syndrome Chin J Tuberc Respir Dis 2015 38 7 524 527 10.3760/cma.j.issn.1001-0939.2015.07.013 WU D D, PAN P H, QIN Q W, et al . Advances in the pathogenesis of acute lung injury/acute respiratory distress syndrome[J ] . Chin J Tuberc Respir Dis, 2015, 38(7):524-527. DOI:10.3760/cma.j.issn.1001-0939.2015.07.013.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

替加环素对患者凝血指标影响的临床研究

金属硫蛋白和锌对急性贫铀中毒大鼠的解毒效果研究

含邻苯二酚基团的黄酮化合物促排急性贫铀中毒效果的研究

贫铀长期摄入对大鼠肾脏损伤效应的研究

对急性贫铀中毒的促排措施研究