DIDS通过抑制氯离子通道2型的开放缓解肝缺血再灌注损伤

邓永; 唐腾骞; 张庆仪; 张雷达

doi:10.16016/j.1000-5404.202007131

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DIDS通过抑制氯离子通道2型的开放缓解肝缺血再灌注损伤

基础医学 | 更新时间：2026-01-09

- DIDS通过抑制氯离子通道2型的开放缓解肝缺血再灌注损伤
- DIDS alleviates hepatic ischemia-reperfusion injury by inhibiting the opening of type 2 chloride channel in rats
- 第三军医大学学报 2020年42卷第24期页码：2375-2381
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)第一附属医院肝胆外科
- 作者简介：
  
  [ 张雷达, E-mail:2518569931@qq.com ZHANG Leida, E-mail:2518569931@qq.com
- 基金信息：
- DOI：10.16016/j.1000-5404.202007131
  中图分类号： R364.12;R657.3;R966
- 收稿：2020-07-15，
  
  修回：2020-09-11，
  
  纸质出版：2020-12-30
- 稿件说明：
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邓永, 唐腾骞, 张庆仪, 等. DIDS通过抑制氯离子通道2型的开放缓解肝缺血再灌注损伤[J]. 第三军医大学学报, 2020,42(24):2375-2381.

Yong DENG, Tengqian TANG, Qingyi ZHANG, et al. DIDS alleviates hepatic ischemia-reperfusion injury by inhibiting the opening of type 2 chloride channel in rats[J]. Journal of Third Military Medical University, 2020, 42(24): 2375-2381.
邓永, 唐腾骞, 张庆仪, 等. DIDS通过抑制氯离子通道2型的开放缓解肝缺血再灌注损伤[J]. 第三军医大学学报, 2020,42(24):2375-2381. DOI： 10.16016/j.1000-5404.202007131.

Yong DENG, Tengqian TANG, Qingyi ZHANG, et al. DIDS alleviates hepatic ischemia-reperfusion injury by inhibiting the opening of type 2 chloride channel in rats[J]. Journal of Third Military Medical University, 2020, 42(24): 2375-2381. DOI： 10.16016/j.1000-5404.202007131.

摘要

目的

探讨氯离子通道2型(type 2 chloride channel

ClC2)及其阻断剂4

4-二异硫氰基芪-2

2’-二磺酸(4

4’-diisothiocyanostilbene-2

2’-disulfonic acid

DIDS)在肝缺血再灌注损伤中的作用。

方法

成年SD大鼠以经典法建立肝缺血再灌注模型。采用q-PCR技术对缺血再灌注后肝组织中ClC2的表达进行检测，采用肝功能血生化分析、活性氧浓度检测、ELISA及流式细胞技术观察缺血再灌注及应用DIDS后对肝功能、氧自由基水平、炎性细胞因子及细胞周期等方面的影响。

结果

缺血再灌注后肝组织中ClC2 mRNA表达显著升高(

0.05)，并伴随有活性氧浓度升高(

0.05)、谷丙及谷草转氨酶升高(

0.05)和炎性细胞因子浓度升高。术后即时使用DIDS组相比缺血再灌注组在ClC2表达、转氨酶浓度、活性氧水平及炎性因子表达方面均有所逆转(

0.05)，术后8 h应用DIDS组相比缺血再灌注组在ClC2表达、转氨酶浓度及iNOS(一氧化氮合酶)表达方面有显著差异(

0.05)，而预用DIDS 1 h组相比缺血再灌注组未观察到统计学差异。

结论

DIDS可以对肝缺血再灌注损伤引起的肝实质损害、炎性反应及氧化应激起保护作用。

Abstract

Objective

To investigate the roles of type 2 chloride channel (ClC2) and its blocker

4'-diisothiocyanostilbene-2

2'-disulfonic acid (DIDS)

in rat model of hepatic ischemia-reperfusion (I/R) injury.

Methods

A total of 75 health adult SD rats were randomly divided into sham operation group

hepatic I/R injury group

I/R+immediate treatment of DIDS group

I/R+8 h later treatment of DIDS group

and 1 h pretreatment of DIDS+I/R group

with 15 animals in each group. The rat model of hepatic I/R injury was established by classical method

and DIDS was given by intraperitoneal injection at a dose of 50 mg/kg at corresponding time points. The expression of ClC2 in the liver tissues was detected with q-PCR. Then blood biochemical analysis

detection of reactive oxygen concentration

ELISA and flow cytometry were employed respectively to analyze and compare the liver function

level of reactive oxygen species (ROS)

contents of inflammatory cytokines and cell cycle of hepatic cells among the different groups.

Results

The rats with hepatic I/R injury had significantly enhanced expression of ClC2 mRNA (

0.05)

increased production of ROS (

0.05)

elevated contents of inflammatory cytokines in liver tissues

and raised serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) (

0.05). However

all above phenomena were reversed in the rats from the I/R+immediate treatment of DIDS group when compared with the model group (

0.05). Significant differences were also observed in the ClC2 expression

transaminase concentrations

and inducible nitric oxide synthase (iNOS) level between the model group and the group using DIDS 8 h after operation (

0.05). But no statistical difference was found in comparison with the group undergoing pretreatment of DIDS 1 h before operation.

Conclusion

DIDS has a protective effect on liver parenchymal damage

inflammatory reaction and oxidative stress induced by hepatic I/R injury.

关键词

Keywords

references

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