浏览全部资源
扫码关注微信
叶酸受体阳性CTC检测在恶性肺部结节辅助诊断中的应用
Application of folate receptor-positive circulating tumor cells in auxiliary diagnosis of malignant pulmonary nodules
- 第三军医大学学报 2020年43卷第23期 页码：2330-2335
- 作者机构：
400037 重庆，陆军军医大学(第三军医大学)第二附属医院病理科
- 作者简介：
[郭乔楠，E-mail:qiaonan85@263.net GUO Qiaonan, E-mail: qiaonan85@263.net
- 基金信息：国家自然科学基金青年科学基金(31801664);利益冲突声明 本研究与CTC检测试剂盒开发公司不存在利益冲突
DOI：10.16016/j.1000-5404.202007074
中图分类号： R446.119;R730.43;R734.2收稿：2020-07-09，
修回：2020-09-24，
纸质出版：2020-12-15
- 稿件说明：
移动端阅览
刘博, 黄露, 曹亚, 等. 叶酸受体阳性CTC检测在恶性肺部结节辅助诊断中的应用[J]. 第三军医大学学报, 2020,43(23):2330-2335.
Bo LIU, Lu HUANG, Ya CAO, et al. Application of folate receptor-positive circulating tumor cells in auxiliary diagnosis of malignant pulmonary nodules[J]. Journal of Third Military Medical University, 2020, 43(23): 2330-2335.
刘博, 黄露, 曹亚, 等. 叶酸受体阳性CTC检测在恶性肺部结节辅助诊断中的应用[J]. 第三军医大学学报, 2020,43(23):2330-2335. DOI： 10.16016/j.1000-5404.202007074.
Bo LIU, Lu HUANG, Ya CAO, et al. Application of folate receptor-positive circulating tumor cells in auxiliary diagnosis of malignant pulmonary nodules[J]. Journal of Third Military Medical University, 2020, 43(23): 2330-2335. DOI： 10.16016/j.1000-5404.202007074.
摘要
目的
2
评价靶向叶酸受体(folate receptor
FR)阳性循环肿瘤细胞(circulating tumor cell
CTC)检测方法在临床肺部结节良恶性诊断中的实用价值。
方法
2
选取2017年5月至2018年4月本院收治的肺部结节筛查患者288例，采用靶向FR CTC技术检测外周血中CTC含量；结合患者术后的病理检查结果，分析靶向FR CTC检测在肺部恶性肿瘤辅助诊断中的准确性、可行性。
结果
2
以8.7 Folate Units/3 mL为cut-off值，136例肺部恶性肿瘤的CTC水平显著高于152例肺部良性疾病患者(
P
<
0.05)。136例肺部恶性肿瘤FR阳性CTC 107例，152例良性病变结节FR假阳性CTC 44例，总体上靶向FR CTC检测技术对肺部恶性肿瘤的检测灵敏度为78.7%，特异性为71.1%。特别是在Ⅰ期肺癌灵敏度达到76.9%，9例原位肺癌CTC检测均呈阳性，与肿瘤标志物、CT检查等比较，CTC检测对早期肺癌具有较高的辅助诊断价值。
结论
2
靶向FR CTC检测可以应用于肺部结节良恶性的筛查，对肺部恶性肿瘤的早发现有较好的辅助诊断价值。
Abstract
Objective
2
To evaluate the practical value of detecting folate receptor (FR)-positive circulating tumor cells (CTC) in clinical diagnosis of benign and malignant lung nodules.
Methods
2
A total of 288 patients with pulmonary nodules admitted in our hospital from May 2017 to April 2018 were enrolled in this study. FR-targeted CTC technology was used to quantitatively detect CTC content in peripheral blood samples. The results were analyzed with the postoperative pathological results to evaluate the accuracy and feasibility of the detection in the auxiliary diagnosis of lung malignant tumors.
Results
2
With a cut-off value of 8.7 Folate Units/3 mL as the threshold
the CTC content was significantly higher in the 136 patients with pulmonary malignant tumor than the 152 patients with pulmonary benign disease (
P
<
0.05). Among the 136 patients with pathologically diagnosed pulmonary malignant tumors
FR-positive CTC were observed in 106 patients. In the 152 patients with benign nodules
44 cases showed false positive CTC results. In general
the sensitivity and specificity of FR-positive CTC detection was 78.7% and 71.1%
respectively in the detection of pulmonary malignant tumors. Furthermore
the sensitivity reached 76.9% in stage I lung cancer (70/91)
and all 9 cases of lung cancer
in situ
were positive in the detection. Compared with tumor markers and CT examinations
FR-positive CTC detection was of higher auxiliary diagnostic value for early lung cancer.
Conclusion
2
FR-positive CTC detection can be used to screen benign and malignant lung nodules
and has a good value in the diagnosis of early-stage pulmonary malignant tumors.
关键词
Keywords
references
李国仁, 戴建华.我国肺部结节精准诊断的现状及研究进展[J].中华胸心血管外科杂志, 2019, 35(9): 566-572. DOI:10.3760/cma.j.issn.1001-4497.2019.09.015.
LI G R, DAI J H. Status and research progress of precision diagnosis of pulmonary nodules[J]. Chin J Thorac Cardiovasc Surg, 2019, 35(9): 566-572. DOI:10.3760/cma.j.issn.1001-4497.2019.09.015.
黄静, 沈庆, 陈元澜, 等.肺部结节管理策略研究进展[J].中国呼吸与危重监护杂志, 2018, 17(1): 97-101. DOI:10.7507/1671-6205.201706020.
HUANG J, SHEN Q, CHEN Y L, et al . Research progress of pulmonary nodule management strategy[J ] . Chin J Respir Crit Care Med, 2018, 17(1): 97-101.DOI:10.7507/1671-6205.201706020.
WANG Y J, GONG J, SUZUKI K, et al . Evidence based imaging strategies for solitary pulmonary nodule[J ] . J Thorac Dis, 2014, 6(7): 872-887. DOI:10.3978/j.issn.2072-1439.2014.07.26.
ZHOU Q, GENG Q, WANG L, et al . Value of folate receptor-positive circulating tumour cells in the clinical management of indeterminate lung nodules: a non-invasive biomarker for predicting malignancy and tumour invasiveness[J ] . EBioMedicine, 2019, 41: 236-243. DOI:10.1016/j.ebiom.2019.02.028.
YU Y, CHEN Z, DONG J, et al . Folate receptor-positive circulating tumor cells as a novel diagnostic biomarker in non-small cell lung cancer[J ] . Transl Oncol, 2013, 6(6): 697-702. DOI:10.1593/tlo.13535.
LOW P S, ANTONY A C. Folate receptor-targeted drugs for cancer and inflammatory diseases[J]. Adv Drug Deliv Rev, 2004, 56(8): 1055-1058. DOI:10.1016/j.addr.2004.02.003.
CHEN X, ZHOU F, LI X, et al . Folate receptor-positive circulating tumour cells as a predictive biomarker for the efficacy of first-line pemetrexed-based therapy in patients with non-squamous non-small cell lung cancer[J ] . Ann Oncol, 2019, 30: v643-v644. DOI:10.1093/annonc/mdz260.084.
YIN W, ZHU J J, MA B T, et al . Overcoming obstacles in pathological diagnosis of pulmonary nodules through circulating tumor cell enrichment[J ] . Small, 2020, 16(25): 2001695. DOI:10.1002/smll.202001695.
BRAY F, FERLAY J, SOERJOMATARAM I, et al . Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J ] . CA Cancer J Clin, 2018, 68(6): 394-424. DOI:10.3322/caac.21492.
SIMMONS J, GOULD M K, IACCARINO J, et al . Systems-level resources for pulmonary nodule evaluation in the United States: a national survey[J ] . Am J Respir Crit Care Med, 2016, 193(9): 1063-1065. DOI:10.1164/rccm.201511-2163le.
PANTEL K, ALIX-PANABIÈRES C. Liquid biopsy and minimal residual disease-latest advances and implications for cure[J]. Nat Rev Clin Oncol, 2019, 16(7): 409-424. DOI:10.1038/s41571-019-0187-3.
REÁTEGUI E, ACETO N, LIM E J, et al . Tunable nanostructured coating for the capture and selective release of viable circulating tumor cells[J ] . Adv Mater, 2015, 27(9): 1593-1599. DOI:10.1002/adma.201404677.
ACETO N, BARDIA A, MIYAMOTO D T, et al . Circulating tumor cell clusters are oligoclonal precursors of breast cancer metastasis[J ] . Cell, 2014, 158(5): 1110-1122. DOI:10.1016/j.cell.2014.07.013.
WAN J C M, MASSIE C, GARCIA-CORBACHO J, et al . Liquid biopsies come of age: towards implementation of circulating tumour DNA[J ] . Nat Rev Cancer,2017, 17(4): 223-238. DOI:10.1038/nrc.2017.7.
LECHARPENTIER A, VIELH P, PEREZ-MORENO P, et al . Detection of circulating tumour cells with a hybrid (epithelial/mesenchymal) phenotype in patients with metastatic non-small cell lung cancer[J ] . Br J Cancer, 2011, 105(9): 1338-1341. DOI:10.1038/bjc.2011.405.
WEI S, GUO C, HE J, et al . Effect of vein-first vs artery-first surgical t echnique on circulating tumor cells and survival in patients with non-small cell lung cancer: a randomized clinical trial and registry-based propensity score matching analysis[J ] . JAMA Surg, 2019, 154(7): e190972. DOI:10.1001/jamasurg.2019.0972.
JIANG T, ZHAO J, ZHAO C, et al . Dynamic monitoring and predictive value of circulating tumor cells in EGFR-mutated advanced non-small-cell lung cancer patients treated with first-line EGFR tyrosine kinase inhibitors[J ] . Clin Lung Cancer, 2019, 20(2): 124-133;e2. DOI:10.1016/j.cllc.2018.11.014.
CHEN X, ZHOU F, LI X, et al . Folate receptor-positive circulating tumor cell detected by LT-PCR-based method as a diagnostic biomarker for non-small-cell lung cancer[J ] . J Thorac Oncol, 2015, 10(8): 1163-1171. DOI:10.1097/jto.0000000000000606.
YI Y. Folate receptor-targeted diagnostics and therapeutics for inflammatory diseases[J]. Immune Netw, 2016, 16(6): 337-343. DOI:10.4110/in.2016.16.6.337.
NUNEZ M I, BEHRENS C, WOODS D M, et al . High expression of folate receptor alpha in lung cancer correlates with adenocarcinoma histology and mutation[J ] . J Thorac Oncol, 2012, 7(5): 833-840. DOI:10.1097/jto.0b013e31824de09c.
ZHANG W, XIAW, LV Z, et al . Liquid biopsy for cancer: circulating tumor cells, circulating free DNA or exosomes?[J ] . Cell Physiol Biochem, 2017, 41(2): 755-768. DOI:10.1159/000458736.
BARRIÈRE G, TARTARY M, RIGAUD M. Epithelial mesenchymal transition: a new insight into the detection of circulating tumor cells[J]. ISRN Oncol, 2012, 2012: 382010. DOI:10.5402/2012/382010.
0
浏览量
0
下载量
0
CSCD
- 网络PDF下载
- Meta-XML下载
- BibTeX下载
- Endnote下载
- RefMan 下载
- NoteExpress下载
- NoteFirst下载
关联资源
相关文章
相关作者
相关机构
- 技术支持由北京北大方正电子有限公司提供 京ICP备09064830号-19
京公网安备11010802024621 - 本系统建议在Chrome、 IE9+ 以上版本浏览器阅读本站内容，360浏览器请切换至极速模式
- Cookies帮助我们提供服务并提供个性化体验。使用本网站，即表示您同意我们使用Cookies
