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3种环状RNA在系统性红斑狼疮中的表达及临床意义
Expression and clinical significance of 3 kinds of circular RNA in systemic lupus erythematosus
- 第三军医大学学报 2020年42卷第24期 页码：2387-2393
- 作者机构：
1.400038 重庆，陆军军医大学第一附属医院皮肤科
2.400038 重庆，陆军军医大学病理生理学教研室
- 作者简介：
[ 游弋，E-mail:youyi_cq@163.com YOU Yi, E-mail:youyi_cq@163.com
- 基金信息：国家自然科学基金面上项目(81673058);重庆市基础科学与前沿技术研究一般项目(cstc2017jcyjAX0251)
DOI：10.16016/j.1000-5404.202007019
中图分类号： R363.21; R394.3; R593.241收稿：2020-07-02，
修回：2020-09-11，
纸质出版：2020-12-30
- 稿件说明：
移动端阅览
郭俊恺, 赵兴旺, 倪兵, 等. 3种环状RNA在系统性红斑狼疮中的表达及临床意义[J]. 第三军医大学学报, 2020,42(24):2387-2393.
Junkai GUO, Xingwang ZHAO, Bing NI, et al. Expression and clinical significance of 3 kinds of circular RNA in systemic lupus erythematosus[J]. Journal of Third Military Medical University, 2020, 42(24): 2387-2393.
郭俊恺, 赵兴旺, 倪兵, 等. 3种环状RNA在系统性红斑狼疮中的表达及临床意义[J]. 第三军医大学学报, 2020,42(24):2387-2393. DOI： 10.16016/j.1000-5404.202007019.
Junkai GUO, Xingwang ZHAO, Bing NI, et al. Expression and clinical significance of 3 kinds of circular RNA in systemic lupus erythematosus[J]. Journal of Third Military Medical University, 2020, 42(24): 2387-2393. DOI： 10.16016/j.1000-5404.202007019.
摘要
目的
2
探讨三种环状RNA(circRNA)在系统性红斑狼疮(systemic lupus erythematosus
SLE)患者中的表达及临床意义，为揭示SLE的临床诊断、治疗提供新的思路和方向。
方法
2
筛选35例SLE患者(实验组)及25例健康对照者(对照组)，提取外周血单个核细胞(PBMCs)，采用反转录定量PCR(RT-qPCR)检测circADCY9，circGARS，circMCTP2的表达水平，分析3种circRNA表达水平与临床活动度指标之间的相关性，采用受试者工作特征曲线(ROC)评价3种circRNA的诊断价值，通过核酸外切酶RNaseR处理检测3种circRNA分子的稳定性及耐受程度，选取circADCY9分子PCR验证其稳定性。
结果
2
① RT-qPCR分析结果显示circADCY9和circGARS在SLE患者PBMCs中表达上调，circMCTP2表达下调(
P
值均
<
0.000 1)；②SLE患者circADCY9，circGARS表达水平与SLE疾病活动指数(SLEDAI)评分呈正相关，与补体C3呈负相关，circMCTP2表达水平与SLEDAI评分呈负相关，与补体C3呈正相关；③SLE患者组和健康对照组circADCY9、circGARS、circMCTP2分子ROC曲线下面积(AUC)分别为0.850 0、0.826 7、0.895 6；④RNaseR处理前后3种circRNA表达情况较为稳定，PCR显示：circRNA相较于mRNA对RNaseR的耐受性更好。
结论
2
SLE患者PBMCs中circADCY9，circGARS，circMCTP2表达明显异常，且稳定性好，与SLE疾病活动度具有相关性。
Abstract
Objective
2
To explore the expression and significance of 3 circular RNAs (circRNA)
that is
circADCY9
circGARS and circMCTP2
in the patients with systemic lupus erythematosus (SLE)
so as to provide new ideas and directions for its clinical diagnosis and treatment.
Methods
2
Peripheral blood mononuclear cells (PBMCs) were extracted from the SLE patients (
n
=35) and healthy volunteers (
n
=25
control group). The expression levels of above 3 circRNAs were detected by real-time quantitative PCR (RT-qPCR)
and their correlations with SLE Disease Activity Index (SLEDAI) were analyzed. The diagnostic value of the 3 circRNAs was evaluated by receiver operating characteristic (ROC) curve. Their stability and tolerance were detected using ribonuclease R (RNaseR) treatment
and the stability of circADCY9 was further verified by PCR analysis.
Results
2
① The levels of circADCY9 and circGARS were up-regulated
while that of circMCTP2 was down-regulated (All
P
values were
<
0.000 1) in the SLE patients than the healthy volunteers. ② In SLE patients
the expression levels of circADCY9 and circGARS were positively correlated with the score of SLEDAI
and negatively with the level of complement C3. By contrast
the circMCTP2 level was negatively correlated with SLEDAI score and positively with complement C3 level. ③ The area under the curve (AUC) for circADCY9
circGARS and circMCTP2 were 0.850 0
0.826 7 and 0.895 6
respectively. ④ The expression levels of the 3 circRNAs remained steady before and after RNaseR treatment
and the result of PCR showed that the tolerance of circADCY9 to RNaseR was better than that of ADCY9 mRNA.
Conclusion
2
The expression of circADCY9
circGARS and circMCTP2 is significantly abnormal in the PBMCs of SLE patients
and shows good stability
which is correlated with the activity of SLE.
关键词
Keywords
references
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