白介素25及其相关因子在非酒精性脂肪肝患者血清及肝内的表达及其意义

吴剑平; 肖海英; 郑雪莲; 张振东; 章燕; 朱萱; 汪安江

doi:10.16016/j.1000-5404.202005286

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白介素25及其相关因子在非酒精性脂肪肝患者血清及肝内的表达及其意义

临床医学 | 更新时间：2026-01-09

- 白介素25及其相关因子在非酒精性脂肪肝患者血清及肝内的表达及其意义
- Expression and significance of interleukin 25 and its related factors in serum and liver of patients with non-alcoholic fatty liver
- 第三军医大学学报 2021年43卷第1期页码：83-90
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金地区科学基金(81460122);江西省杰出青年人才资助计划(20171BCB23085);江西省研究生创新基金(YC2018-S095)
- DOI：10.16016/j.1000-5404.202005286
  中图分类号：
- 收稿：2020-05-30，
  
  纸质出版：2021-01-15
- 稿件说明：
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吴剑平, 肖海英, 郑雪莲, 等. 白介素25及其相关因子在非酒精性脂肪肝患者血清及肝内的表达及其意义[J]. 第三军医大学学报, 2021,43(1):83-90.

Jianping WU, Haiying XIAO, Xuelian ZHENG, et al. Expression and significance of interleukin 25 and its related factors in serum and liver of patients with non-alcoholic fatty liver[J]. Journal of Third Military Medical University, 2021, 43(1): 83-90.
吴剑平, 肖海英, 郑雪莲, 等. 白介素25及其相关因子在非酒精性脂肪肝患者血清及肝内的表达及其意义[J]. 第三军医大学学报, 2021,43(1):83-90. DOI： 10.16016/j.1000-5404.202005286.

Jianping WU, Haiying XIAO, Xuelian ZHENG, et al. Expression and significance of interleukin 25 and its related factors in serum and liver of patients with non-alcoholic fatty liver[J]. Journal of Third Military Medical University, 2021, 43(1): 83-90. DOI： 10.16016/j.1000-5404.202005286.

摘要

Abstract

Objective

To investigate the expression levels of interleukin 25 (IL-25) and its related factors in the serum and liver of patients with non-alcoholic fatty liver disease (NAFLD) and explore the significance.

Methods

Between September 2017 and February 2019

NAFLD patients (

=50) who met the criteria of the disease and control patients (

=20) who undergoing partial hepatectomy due to hepatic hemangioma or hepatic cyst in the First Affiliated Hospital of Nanchang University were enrolled in this study. Serum levels of IL-25 and other cytokines were detected by ELISA. Then 6 patients in control group and 12 patients in NAFLD group were chosen to analyze hepatic mRNA expression levels of IL-25 and other cytokines by real-time fluorescent quantitative PCR

and protein levels of IL-25

CD68 (macrophage marker)

CD206 (M2 macrophage marker) and glutaminase transaminase 2 (TGM2) by immunohistochemistry or immunofluorescence assay.

Results

① Compared with the patients in control group

those of th

e NAFLD group had significantly reduced serum levels of IL-25 (

=0.003)

IL-13 (

=0.007) and IL-4 (

=0.001). ② The mRNA levels of IL-13 (

=0.007) and IL-4 (

=0.003) in the liver of NAFLD patients were also obviously lower while that of CD68 (

0.001) was higher than those in the control group. ③ The NAFLD patients had more Küpffer cells (

0.001)

fewer M2 macrophages (

0.001) and lower level of IL-25 (

0.001) in the liver than the patietns of the control group. With the aggravation of hepatic steatosis

the number of Küpffer cells was increased gradually (

0.001)

while that of M2 macrophages (

0.001) and the level of IL-25 (

0.001) was decreased gradually.

Conclusion

The serum levels and liver contents of IL-25

Th2 type cytokine and intrahepatic M2 type macrophages are significantly reduced in the NAFLD patients

and these indicators are negatively correlated with the severity of liver steatosis.

关键词

Keywords

references

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