胆汁淤积性肝病患者肠道菌群特征及其临床相关性研究

伍灵怡; 唐黎; 张静; 雷媛媛; 胡世平; 陈雨琳; 喻晓刚; 王应邦; 唐波; 杨国栋

doi:10.16016/j.1000-5404.202005245

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胆汁淤积性肝病患者肠道菌群特征及其临床相关性研究

临床医学 | 更新时间：2026-01-09

- 胆汁淤积性肝病患者肠道菌群特征及其临床相关性研究
- Characteristics of gut microbiota and their correlation with clinical parameters in patients with cholestatic liver disease
- 第三军医大学学报 2020年42卷第22期页码：2251-2258
- 作者机构：
  
  1.637000 四川南充，川北医学院附属医院消化内科
  2.400037 重庆，陆军军医大学(第三军医大学)第二附属医院消化内科
- 作者简介：
  
  [ 唐波，E-mail:atreebobo@163.com TANG Bo, E-mail: atreebobo@163.com
  [ 杨国栋，E-mail:ygd_ld2003@163.com YANG Guodong, E-mail:ygd_ld2003@163.com
- 基金信息：
  
  南充市科技项目市校科技战略合作专项(18SHZ0371)
- DOI：10.16016/j.1000-5404.202005245
  中图分类号： R322.45;R378;R575
- 收稿：2020-05-26，
  
  修回：2020-08-22，
  
  纸质出版：2020-11-30
- 稿件说明：
移动端阅览
伍灵怡, 唐黎, 张静, 等. 胆汁淤积性肝病患者肠道菌群特征及其临床相关性研究[J]. 第三军医大学学报, 2020,42(22):2251-2258.

Lingyi WU, Li TANG, Jing ZHANG, et al. Characteristics of gut microbiota and their correlation with clinical parameters in patients with cholestatic liver disease[J]. Journal of Third Military Medical University, 2020, 42(22): 2251-2258.
伍灵怡, 唐黎, 张静, 等. 胆汁淤积性肝病患者肠道菌群特征及其临床相关性研究[J]. 第三军医大学学报, 2020,42(22):2251-2258. DOI： 10.16016/j.1000-5404.202005245.

Lingyi WU, Li TANG, Jing ZHANG, et al. Characteristics of gut microbiota and their correlation with clinical parameters in patients with cholestatic liver disease[J]. Journal of Third Military Medical University, 2020, 42(22): 2251-2258. DOI： 10.16016/j.1000-5404.202005245.

摘要

目的

分析胆汁淤积性肝病的肠道菌群多样性和结构组成及其与临床参数间的相关性。

方法

收集胆汁淤积性肝病患者(

=16)和健康人群对照者(

=19)的新鲜粪便，采用16S rRNA分析受试者肠道菌群的多样性和结构特征，运用Pearson法分析差异菌属与临床参数间的相关性。

结果

基线资料显示研究对象的性别、年龄、BMI无统计学差异(

0.05)，血清学指标有统计学差异(

0.01)。α多样性表明

胆汁淤积性肝病组的Chao1和Shannon指数明显减少，但无统计学差异，β多样性有统计学差异(

0.01)；菌群结构分析发现

Bacteroides

(拟杆菌属)、

Lachnoclostridium

、

Veillonella

(韦荣氏球菌属)、

Roseburia

(罗斯氏菌属)、

Escherichia_shigella

(大肠埃希菌-志贺氏菌属)在胆汁淤积性肝病组中明显富集(

0.05)，而

Blautia

(布劳特氏菌属)明显减少(

0.05)。Pearson法分析表明，

Lachnoclostridium

与ALP(

0.01)、GGT(

0.05)强相关，

Veillonella

(韦荣氏菌属)与GGT强相关(

0.05)。

结论

胆汁淤积性肝病患者与健康人群对照者相比，肠道菌群存在特征性改变，

Veillonella

(韦荣氏球菌属)相对高丰度表达与胆汁淤积性肝病强相关。

Abstract

Objective

To investigate the diversity and composition of gut microbiota in patient with cholestatic liver disease

and explore their correlation with clinical parameters.

Methods

Fresh stool samples from 16 patients with cholestatic liver disease and 19 healthy controls were collected

and 16S rRNA sequencing was used to analyze the diversity of gut microbiota and composition characteristics. Pearson analysis was employed to analyze the correlation between the different bacteria and clinical parameters.

Results

There were no statistical differences in the baseline data

such as sex

age and body mass index (BMI) in study subjects (

0.05)

but significant differences were observed in serum indexes among them (

0.01). The results of α diversity showed that Chao1 and Shannon indexes were lower in the study group

but there was no statistical difference (

0.05). β diversity results indicated that the species diversity had significant difference between 2 group (

0.01). Further analysis of microbiota composition showed largely relative abundance of

Bacteroides

Lachnoclostridium

Veillonella

Roseburia

and

Escherichia shigella

(

0.05)

while the abundance of

Blautia

were dramatically decreased (

0.05). Pearson analysis displayed that

Lachnoclostridium

was strongly correlated with serum level of alkaline phosphatase (ALP) (

0.01) and gamma-glutamyl transferase (GGT) (

0.05)

and

Veillonella

was also strongly correlated with GGT (

0.05).

Conclusion

Compared with healthy controls

the patients with cholestatic liver disease have obvious characteristic changes in the gut microbiota. The relatively high abundance of

Veillonella

is closely assocatied with cholestatic liver disease.

关键词

Keywords

references

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