复方嗜酸乳杆菌片对便秘的治疗作用及对肠道菌群的影响

周凯旋; 鲍慧玮; 朱志杰; 李丽静

doi:10.16016/j.1000-5404.202005171

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复方嗜酸乳杆菌片对便秘的治疗作用及对肠道菌群的影响

药学 | 更新时间：2026-01-09

- 复方嗜酸乳杆菌片对便秘的治疗作用及对肠道菌群的影响
- Therapeutic effect of compound Lactobacillus acidophilus tablets on constipation and its impact on intestinal flora in mice
- 第三军医大学学报 2020年42卷第20期页码：2031-2038
- 作者机构：
  
  1.130117 长春，长春中医药大学药学院
  2.134003 吉林通化，通化金马药业集团股份有限公司研发部
- 作者简介：
  
  [李丽静，E-mail:lilijing66@163.com LI Lijing, E-mail: lilijing66@163.com
- 基金信息：
  
  吉林省科技成果转化计划项目(201603017YY);研究生教育指导委员会课题(20190723-FJ-B33);利益冲突声明在课题研究和文章撰写过程，没有因其岗位角色影响文章观点和对数据结果的报道，不存在利益冲突
- DOI：10.16016/j.1000-5404.202005171
  中图分类号： R322.45;R378.992;R442.2
- 收稿：2020-05-20，
  
  修回：2020-06-22，
  
  纸质出版：2020-10-30
- 稿件说明：
移动端阅览
周凯旋, 鲍慧玮, 朱志杰, 等. 复方嗜酸乳杆菌片对便秘的治疗作用及对肠道菌群的影响[J]. 第三军医大学学报, 2020,42(20):2031-2038.

Kaixuan ZHOU, Huiwei BAO, Zhijie ZHU, et al. Therapeutic effect of compound Lactobacillus acidophilus tablets on constipation and its impact on intestinal flora in mice[J]. Journal of Third Military Medical University, 2020, 42(20): 2031-2038.
周凯旋, 鲍慧玮, 朱志杰, 等. 复方嗜酸乳杆菌片对便秘的治疗作用及对肠道菌群的影响[J]. 第三军医大学学报, 2020,42(20):2031-2038. DOI： 10.16016/j.1000-5404.202005171.

Kaixuan ZHOU, Huiwei BAO, Zhijie ZHU, et al. Therapeutic effect of compound Lactobacillus acidophilus tablets on constipation and its impact on intestinal flora in mice[J]. Journal of Third Military Medical University, 2020, 42(20): 2031-2038. DOI： 10.16016/j.1000-5404.202005171.

摘要

目的

通过动物实验和细菌16S rRNA基因高通量测序，研究复方嗜酸乳杆菌片对便秘的治疗作用和对肠道菌群的影响。

方法

采用复方地芬诺酯片对小鼠进行便秘造模，给予复方嗜酸乳杆菌片治疗，阳性对照药为酚酞片，测量小鼠的小肠运动能力和粪便含水量，进行病理切片，观察结肠病理变化，使用16s rRNA基因测序研究药物对肠道菌群的影响。

结果

小肠运动情况表明:复方嗜酸乳杆菌片和酚酞能明显提升便秘小鼠的碳墨推进率和粪便含水量；结肠病理切片表明:模型组结肠损伤严重，给药各组有不同程度改善；16S rRNA测序结果显示:便秘模型动物物种丰富度相较于正常组增多，在门水平上，拟杆菌门和厚壁菌门发生显著性变化，在属水平上，有7个属发生显著性变化，复方嗜酸乳杆菌片对肠道菌群失调有所改善，而酚酞片对肠道菌群几乎无调节作用。

结论

复方嗜酸乳杆菌片对便秘具有治疗作用，并对肠道菌群具有调节作用。

Abstract

Objective

To evaluate the therapeutic effect of compound

Lactobacillus acidophilus

tablets on constipation and its impact on intestinal flora in mice by animal experiments and 16S rRNA gene sequencing.

Methods

In a mouse model of constipation induced by treatment with compound diphenoxylate tablets

we evaluated the therapeutic effect of compound

Lactobacillus acidophilus

tablets in comparison with the positive control drug phenolphthalein tablets by examining small intestinal motility

fecal water content

and colon pathologies. 16S rRNA gene sequencing was performed to investigate the effect of the treatment on intestinal flora of the mice.

Results

Treatment with compound

Lactobacillus acidophilus

tablets and phenolphthalein both significantly accelerated the rate of carbon ink advancement and increased fecal water content in mice with constipation. Pathological examination of the colon sections revealed severe colon injury in the model group

which was improved by treatment with the 2 test drugs. The results of 16S rRNA sequencing showed that in mice with constipation

the species richness in the intestinal flora was increased significantly as compared with that in normal mice

with significant changes in 7 genera and in 2 phyla (Bacteroides and Firmicutes). Treatment with compound

Lactobacillus acidophilus

tablets ameliorated these changes in intestinal flora

while phenolphthalein tablets produced no such regulatory effect.

Conclusion

Compound

Lactobacillus acidophilus

tablets have obvious therapeutic effects on constipation and show regulatory effects on intestinal flora in mice.

关键词

Keywords

references

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