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Notch通路参与调控成年小鼠c-kit+视网膜前体细胞分裂和分化修复NMDA视网膜损伤
Notch pathway participates in regulation of c-kit+ retinal precursor cell division and differentiation in adult mice for repair of NMDA-induced retinal injury
- 第三军医大学学报 2021年43卷第1期 页码：59-67
- 作者机构：
- 作者简介：
- 基金信息：国家自然科学基金面上项目(81870686);北京市自然科学基金青年项目(7184201);首都卫生发展科研专项(首发2018-1-2021)
DOI：10.16016/j.1000-5404.202005147
中图分类号：收稿：2020-05-18，
纸质出版：2021-01-15
- 稿件说明：
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陈曦, 李珊珊, 赵露, 等. Notch通路参与调控成年小鼠c-kit+视网膜前体细胞分裂和分化修复NMDA视网膜损伤[J]. 第三军医大学学报, 2021,43(1):59-67.
Xi CHEN, Shanshan LI, Lu ZHAO, et al. Notch pathway participates in regulation of c-kit+ retinal precursor cell division and differentiation in adult mice for repair of NMDA-induced retinal injury[J]. Journal of Third Military Medical University, 2021, 43(1): 59-67.
陈曦, 李珊珊, 赵露, 等. Notch通路参与调控成年小鼠c-kit+视网膜前体细胞分裂和分化修复NMDA视网膜损伤[J]. 第三军医大学学报, 2021,43(1):59-67. DOI： 10.16016/j.1000-5404.202005147.
Xi CHEN, Shanshan LI, Lu ZHAO, et al. Notch pathway participates in regulation of c-kit+ retinal precursor cell division and differentiation in adult mice for repair of NMDA-induced retinal injury[J]. Journal of Third Military Medical University, 2021, 43(1): 59-67. DOI： 10.16016/j.1000-5404.202005147.
摘要
Abstract
Objective
2
To analyze the characteristics of c-kit
+
endogenous retinal precursor cells (RPCs) in the retina of adult mice and their roles in the repair of N-methyl-D-aspartic acid (NMDA)-induced retinal damage.
Methods
2
Mouse models of NMDA-induced retinal injury were established in 4-week-old adult C57BL/6J mice. Immunofluorescence assay was used to analyze the relationship between c-kit
+
RPCs and c-kit stem cell factor (SCF)-positive cells and determine the number of c-kit
+
RPCs and their division activity at 1
2 and 4 weeks after NMDA injury. The mRNA expression levels of Hes1
Hes5
S100b and Pou4f1 in the injured retinal tissues were analyzed using real-time PCR.
Results
2
SC
F
+
Müller cells surrounding the c-kit
+
RPCs were observed in the inner nuclear layer (INL) of the retina of adult mice. C-kit
+
RPCs still maintained a low level of cell division activity. Two weeks after NMDA-induced retinal injury
the number of c-kit
+
RPCs significantly increased in the retina (
P
<
0.001) with markedly increased proportions of dividing cells (
P
=0.016) and c-kit
+
GAD65/67
+
cells/c-kit
+
cells (
P
<
0.001). Two weeks after retinal injury
the Notch pathway components Hes1 and Hes5 were up-regulated (
P
<
0.001) and the expression levels of S100b and Pou4f1 were augmented significantly in the retina (
P
<
0.001).
Conclusion
2
Two weeks after NMDA-induced retinal injury
c-kit
+
RPCs in the retina undergo both symmetrical and asymmetrical cell division and then differentiate into GAD65/67
+
neurons possibly under regulation by the Notch pathway.
关键词
Keywords
references
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