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磺化聚醚醚酮-他汀缓释系统促进骨修复的作用研究
Sulfonated PEEK-statins delivery system enhances bone repair in mice with skull defect
- 第三军医大学学报 2020年42卷第19期 页码：1907-1912
- 作者机构：
- 作者简介：
- 基金信息：国家自然科学基金(81403029);上海市科学技术委员会面上项目(19ZR1449100)
DOI：10.16016/j.1000-5404.202005009
中图分类号：收稿：2020-05-03，
纸质出版：2020-10-15
- 稿件说明：
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吕丹, 孙莹霄, 欧阳丽萍, 等. 磺化聚醚醚酮-他汀缓释系统促进骨修复的作用研究[J]. 第三军医大学学报, 2020,42(19):1907-1912.
Dan LYU, Yingxiao SUN, Liping OUYANG, et al. Sulfonated PEEK-statins delivery system enhances bone repair in mice with skull defect[J]. Journal of Third Military Medical University, 2020, 42(19): 1907-1912.
吕丹, 孙莹霄, 欧阳丽萍, 等. 磺化聚醚醚酮-他汀缓释系统促进骨修复的作用研究[J]. 第三军医大学学报, 2020,42(19):1907-1912. DOI： 10.16016/j.1000-5404.202005009.
Dan LYU, Yingxiao SUN, Liping OUYANG, et al. Sulfonated PEEK-statins delivery system enhances bone repair in mice with skull defect[J]. Journal of Third Military Medical University, 2020, 42(19): 1907-1912. DOI： 10.16016/j.1000-5404.202005009.
摘要
Abstract
Objective
2
To explore the effect of sulfonated poly-ether-ether-ketone (SPEEK)-statin delivery system on the repair of bone defect
H-type blood vessels and bone formation.
Methods
2
The mouse model of skull defect was established
SPEEK-pravastatin (SPEEK-Pra) and sulfonated SPEEK-simvastatin (SPEEK-Sim) delivery system were constructed
respectively. Then the samples with different concentration gradients (SPEEK 0.16 mg/mL
SPEEK -Pra 0.16 mg/mL
SPEEK -Sim 0.55 mg/mL
SPEEK -Sim 1.1 mg/mL
SPEEK -Sim 2.2 mg/mL) were implanted into the skull defect of mice
respectively. After 8 weeks
the skull samples were collected
and micro-CT scanning was used to observe the bone regeneration around of the defects
and CD31 and EMCN immunofluorescent staining was utilized to label H-type blood vessels.
Results
2
SPEEK can be used as a payload for statins. Micro-CT results showed that the thickness of skull were significantly larger in SPEEK-sim 1.1 mg/mL and SPEEK-sim 0.55 mg /mL groups than the other 3 groups
with that of the SPEEK group (without drug loading) lowest. Immunofluorescence assay found that the double-positive area was clearly visible in the SPEEK-sim 1.1 mg /mL group
but not observed in the SPEEK group and the SPEEK-pra 0.16 mg/mL group.
Conclusion
2
SPEEK-statin sustained-release system optimizes the osteogenic properties of SPEEK
which may be related to the protective effect of H blood vessel.
关键词
Keywords
references
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