周细胞对失血性休克大鼠血管通透性的保护作用

何霜霜; 朱娱; 周赫男; 王洪晨; 李涛; 刘良明

doi:10.16016/j.1000-5404.202004227

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周细胞对失血性休克大鼠血管通透性的保护作用

军事医学(2019年度重庆市出版专项资金资助项目) | 更新时间：2026-01-09

- 周细胞对失血性休克大鼠血管通透性的保护作用
- Protective effect of pericytes against vascular leakage in rats with hemorrhagic shock
- 第三军医大学学报 2020年42卷第19期页码：1882-1889
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金重点项目(81830065)
- DOI：10.16016/j.1000-5404.202004227
  中图分类号：
- 收稿：2020-04-21，
  
  纸质出版：2020-10-15
- 稿件说明：
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何霜霜, 朱娱, 周赫男, 等. 周细胞对失血性休克大鼠血管通透性的保护作用[J]. 第三军医大学学报, 2020,42(19):1882-1889.

Shuangshuang HE, Yu ZHU, Henan ZHOU, et al. Protective effect of pericytes against vascular leakage in rats with hemorrhagic shock[J]. Journal of Third Military Medical University, 2020, 42(19): 1882-1889.
何霜霜, 朱娱, 周赫男, 等. 周细胞对失血性休克大鼠血管通透性的保护作用[J]. 第三军医大学学报, 2020,42(19):1882-1889. DOI： 10.16016/j.1000-5404.202004227.

Shuangshuang HE, Yu ZHU, Henan ZHOU, et al. Protective effect of pericytes against vascular leakage in rats with hemorrhagic shock[J]. Journal of Third Military Medical University, 2020, 42(19): 1882-1889. DOI： 10.16016/j.1000-5404.202004227.

摘要

Abstract

Objective

To investigate the protective effect of pericytes (PCs) against vascular leakage in rats with hemorrhagic shock.

Methods

Sixty-four 12- to 24-week-old SD rats weighing 200~220 g were randomized equally into sham-operated group

hemorrhagic shock group (HS group)

Ringer's lactate solution treatment group (LR group)

and PC treatment group (PC group). In the latter 3 groups

rat models of hemorrhagic shock were established by lowering the blood pressure to 30 mmHg

which was maintained for 3 h. Tissue samples were collected 12 h after corresponding treatment for determination of the number of pericytes

vascular permeability of the pulmonary vessels and the mesenteric microvessels

and expressions of zonula occludens 1 (ZO-1) and VE-cadherin.

Results

The vascular permeability was increased (

0.05) and the number of pericytes and expressions of ZO-1 and VE-cadherin were decreased significantly (

0.05) in rats with hemorrhagic shock compared with the sham-operated rats. At 12 h after LR treatment

the vascular permeability was slightly reduced

the tight junction between the endothelial cells was moderately improved and the expression levels of ZO-1 and VE-cadherin were mildly increased in rats with hemorrhagic shock. At 12 h after PC treatment

the number of pericytes in the pulmonary vein was increased

the cell morphology and tight junction between the endothelial cells were restored

and the expressions of ZO-1 and VE-cadherin were increased significantly to nearly the normal levels in rats with hemorrhagic shock (

0.05); the mesenteric microvascular permeability and vascular leakage were improved significantly after treatment with PCs.

Conclusion

PC infusion produces significantly protective effect against vascular leakage in rats with hemorrhagic shock.

关键词

Keywords

references

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