抗菌肽ET12对革兰阳性菌的抗菌活性及机制研究

袁慊; 冯伟; 王瑜; 姚璞; 孙凤军

doi:10.16016/j.1000-5404.202003346

您当前的位置：

首页 >

文章列表页 >

抗菌肽ET12对革兰阳性菌的抗菌活性及机制研究

药学 | 更新时间：2026-01-09

- 抗菌肽ET12对革兰阳性菌的抗菌活性及机制研究
- Antibacterial activity and mechanism of antimicrobial peptide ET12 against Gram-positive bacteria
- 第三军医大学学报 2020年42卷第15期页码：1567-1572
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)第一附属医院药剂科
- 作者简介：
  
  [ 孙凤军，E-mail: fengj_sun@163.com SUN Fengjun, E-mail:fengj_sun@163.com
- 基金信息：
  
  国家重点研发计划项目(2017YFC1200400)
- DOI：10.16016/j.1000-5404.202003346
  中图分类号： R378;R965;R977.6
- 收稿：2020-03-26，
  
  修回：2020-05-09，
  
  纸质出版：2020-08-15
- 稿件说明：
移动端阅览
袁慊, 冯伟, 王瑜, 等. 抗菌肽ET12对革兰阳性菌的抗菌活性及机制研究[J]. 第三军医大学学报, 2020,42(15):1567-1572.

Qian YUAN, Wei FENG, Yu WANG, et al. Antibacterial activity and mechanism of antimicrobial peptide ET12 against Gram-positive bacteria[J]. Journal of Third Military Medical University, 2020, 42(15): 1567-1572.
袁慊, 冯伟, 王瑜, 等. 抗菌肽ET12对革兰阳性菌的抗菌活性及机制研究[J]. 第三军医大学学报, 2020,42(15):1567-1572. DOI： 10.16016/j.1000-5404.202003346.

Qian YUAN, Wei FENG, Yu WANG, et al. Antibacterial activity and mechanism of antimicrobial peptide ET12 against Gram-positive bacteria[J]. Journal of Third Military Medical University, 2020, 42(15): 1567-1572. DOI： 10.16016/j.1000-5404.202003346.

摘要

目的

研究抗菌肽ET12的抗菌活性及机制。

方法

通过微量肉汤稀释法检测抗菌肽ET12对临床常见菌株的最低抑菌浓度(MIC)，激光共聚焦显微镜观察抗菌肽ET12对表皮葡萄球菌生物膜形成和细胞膜通透性的影响，透射电镜观察抗菌肽ET12对表皮葡萄球菌细胞形态的影响，利用脱纤维绵羊血检测抗菌肽ET12的溶血活性，利用CCK-8试剂盒检测抗菌肽ET12对细胞的毒性。

结果

抗菌肽ET12对革兰阳性菌特别是表皮葡萄球菌具有良好的抗菌活性，MIC值在8~64 μg/mL之间。ET12能够有效抑制表皮葡萄球菌生物膜的形成及破坏细菌细胞形态，增强细胞膜通透性。在有效抗菌浓度范围内，ET12对绵羊红细胞的溶血率小于2.5%，RAW264.7细胞存活率大于80%。

结论

抗菌肽ET12对革兰阳性菌具有较强的抗菌活性，细胞膜可能是其抗菌作用靶点，且溶血活性和细胞毒性较低。

Abstract

Objective

To study the antibacterial activity and mechanism of antimicrobial peptide ET12.

Methods

The minimal inhibitory concentration (MIC) of ET12 on clinical common strains was determined by broth dilution method. The effect of ET12 on the biofilm formation and membrane permeability of

Staphylococcus epidermidis

(

S. epidermidis

) was observed by confocal laser microscopy. The effect of ET12 on the cell morphology was observ

ed by transmission electron microscopy. Defibrillation sheep blood was used to detect the hemolytic activity of ET12. Cell counting Kit-8 assay was used to detect the cytotoxicity of ET12.

Results

ET12 had good antibacterial activity against Gram-positive bacteria

especially

S. epidermidis

. The MIC values of ET12 against Gram-positive bacteria were between 8 and 64 μg/mL. ET12 could inhibit the biofilm formation of

S. epidermidis

damage the bacterial morphology and enhance cell membrane permeability. Within the effective concentration ranges of antibacterial activity

the hemolysis rate of ET12 on sheep red blood cells was less than 2.5%

and the survival rate of RAW264.7 cells was above 80%.

Conclusion

ET12 has strong antibacterial activity against Gram-positive bacteria

with the cell membrane as target

and has low hemolytic activity and cytotoxicity.

关键词

Keywords

references

MORAVEJ H, MORAVEJ Z, YAZDANPARAST M, et al . Antimicrobial peptides: features, action, and their resistance mechanisms in bacteria[J ] . Microb Drug Resist, 2018, 24(6): 747-767. DOI:10.1089/mdr.2017.0392.

李冠楠, 夏雪娟, 隆耀航, 等.抗菌肽的研究进展及其应用[J].动物营养学报, 2014, 26(1): 17-25. DOI: 10.3969/j.issn.1006-267x.2014.01.003.

LI G N, XIA X J, LONG Y H, et al . Research progress and application of antimicr obial peptides[J ] . Chin J Animal Nutrit, 2014, 26(1): 17-25. DOI: 10.3969/j.issn.1006-267x.2014.01.003.

KOCZULLA A R, BALS R. Antimicrobial peptides: current status and therapeutic potential[J]. Drugs, 2003, 63(4): 389-406. DOI:10.2165/00003495-200363040-00005.

NGUYEN L T, HANEY E F, VOGEL H J. The expanding scope of antimicrobial peptide structures and their modes of action[J]. Trends Biotechnol, 2011, 29(9): 464-472. DOI:10.1016/j.tibtech.2011.05.001.

BASTOS P, TRINDADE F, DA COSTA J, et al . Human antimicrobial peptides in bodily fluids: current knowledge and therapeutic perspectives in the postantibiotic era[J ] . Med Res Rev, 2018, 38(1): 101-146. DOI:10.1002/med.21435.

彭博, 郭中敏, 陆家海.人工合成抗菌肽的常用方法及应用前景[J].中国抗生素杂志, 2012, 37(3): 176-183. DOI:10.13461/j.cnki.cja.004954.

PENG B, GUO Z M, LU J H. The common methods and application prospect of the artificial synthetic antibacterial peptide[J]. Chin J Antibiot, 2012, 37(3): 176-183. DOI:10.13461/j.cnki.cja.004954.

CHEN Z H, CHANG D, ZOU Y Q, et al . Genome sequence of Enterococcus faeciu m clinical isolate LCT-EF128[J ] . J Bacteriol, 2012, 194(17): 4765. DOI:10.1128/JB.00996-12.

OUYANG J, SUN F J, FENG W, et al . Antimicrobial activity of galangin and its effects on murein hydrolases of vancomycin-intermediate Staphylococcus aureus (VISA) strain Mu50[J ] . Chemotherapy, 2018, 63(1): 20-28. DOI:10.1159/000481658.

WANG Q, XU Y Z, DONG M M, et al . HJH-1, a broad-spectrum antimicrobial activity and low cytotoxicity antimicrobial peptide[J ] . Molecules, 2018, 23(8): E2026. DOI:10.3390/molecules23082026.

JIA F J, ZHANG Y, WANG J Y, et al . The effect of halogenation on the antimicrobial activity, antibiofilm activity, cytotoxicity and proteolytic stability of the antimicrobial peptide Jelleine-I[J ] . Peptides, 2019, 112: 56-66. DOI:10.1016/j.peptides.2018.11.006.

VOGEL H J, SCHIBLI D J, JING W G, et al . Towards a structure-function analysis of bovine lactoferricin and related tryptophan- and arginine-containing peptides[J ] . Biochimie Et Biol Cell, 2002, 80(1): 49-63. DOI:10.1139/o01-213.

李国栋, 钱承军, 陆敏, 等.一组人工合成抗菌肽的研究[J].微生物学报, 2006, 46(3): 496-499. DOI:10.13343/j.cnki.wsxb.2006.03.033.

LI G D, QIAN C J, LU M, et al . A group of synthetic antimicrobial peptides[J ] . Acta Microbiol Sin, 2006, 46(3): 496-499. DOI:10.13343/j.cnki.wsxb.2006.03.033.

OMARDIEN S, BRUL S, ZAAT S A J. Antimicrobial activity of cationic antimicrobial peptides against gram-positives: current progress made in understanding the mode of action and the response of bacteria[J]. Front Cell Dev Biol, 2016, 4:111. DOI: 10.3389/fcell.2016.00111.

GRUENHEID S, LE MOUAL H. Resistance to antimicrobial peptides in Gram-negative bacteria[J]. FEMS Microbiol Lett, 2012, 330(2): 81-89. DOI: 10.1111/j.1574-6968.2012.02528.x.

SUN F J, QU F, LING Y, et al . Biofilm-associated infections: antibiotic resistance and novel therapeutic strategies[J ] . Future Microbiol, 2013, 8(7): 877-886. DOI:10.2217/fmb.13.58.

BOLINTINEANU D, HAZRATI E, DAVIS H T, et al . Antimicrobial mechanism of pore-forming protegrin peptides: 100 pores to kill E. coli[J ] . Peptides, 2010, 31(1): 1-8. DOI:10.1016/j.peptides.2009.11.010.

MATSUZAKI K, NAKAMURA A, MURASE O, et al . Modulation of Magainin 2-lipid bilayer interactions by peptide charge[J ] . Biochemistry, 1997, 36(8): 2104-2111. DOI:10.1021/bi961870p.

WANG K, DANG W, YAN J, et al . Membrane perturbation action mode and structure-activity relationships of protonectin, a novel antimicrobial peptide from the venom of the neotropical social wasp agelaia pallipes pallipes[J ] . Antimicrob Agents Chemother, 2013, 57(10): 4632-4639. DOI: 10.1128/AAC.02311-12.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

司帕沙星对类风湿关节炎成纤维样滑膜细胞的声动力细胞毒性

IR-780联合阿霉素对肝癌干细胞样细胞的杀伤效应研究

大黄素抑制MRSA生物膜活性及其作用机制研究

昼夜节律分子BMAL1通过调节免疫细胞毒性促进骨肉瘤的预后

亚抑菌浓度亚胺培南通过影响Ⅳ型菌毛增强耐药鲍曼不动杆菌的生物膜形成