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新型CD45阴性细胞分选技术在多发性骨髓瘤细胞遗传学检测中的临床应用
Clinical application of a novel CD45-negative cell sorting in cytogenetic detection of multiple myeloma
- 第三军医大学学报 2020年42卷第17期 页码：1681-1686
- 作者机构：
- 作者简介：
- 基金信息：重庆市技术创新与应用示范(社会民生类)一般项目资助(CSTC2018jscx-msybX0045)
DOI：10.16016/j.1000-5404.202003335
中图分类号：收稿：2020-03-26，
纸质出版：2020-09-15
- 稿件说明：
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陈果, 曾韫璟, 王平, 等. 新型CD45阴性细胞分选技术在多发性骨髓瘤细胞遗传学检测中的临床应用[J]. 第三军医大学学报, 2020,42(17):1681-1686.
Guo CHEN, Yunjing ZENG, Ping WANG, et al. Clinical application of a novel CD45-negative cell sorting in cytogenetic detection of multiple myeloma[J]. Journal of Third Military Medical University, 2020, 42(17): 1681-1686.
陈果, 曾韫璟, 王平, 等. 新型CD45阴性细胞分选技术在多发性骨髓瘤细胞遗传学检测中的临床应用[J]. 第三军医大学学报, 2020,42(17):1681-1686. DOI： 10.16016/j.1000-5404.202003335.
Guo CHEN, Yunjing ZENG, Ping WANG, et al. Clinical application of a novel CD45-negative cell sorting in cytogenetic detection of multiple myeloma[J]. Journal of Third Military Medical University, 2020, 42(17): 1681-1686. DOI： 10.16016/j.1000-5404.202003335.
摘要
Abstract
Objective
2
To explore the clinical value of a novel CD45-negative cell sorting technology in sorting and enriching tumor plasma cells in multiple myeloma (MM).
Methods
2
A total of 60 newly diagnosed MM patients from 3 hospitals in Chongqing from July 2018 to December 2019 were recruited in this study. Bone marrow samples of 3~4 mL were taken from each patient and divided into 2 parts. One part (direct detection group) was directly detected by flow cytometry and FISH
and the other part (CD45 sorting group) was subjected to the novel CD45-negative cell sorting
followed by flow cytometry and FISH. The proportion of plasma cells in bone marrow
detection rate of cytogenetic abnormalities and risk stratification were compared in every patient before and after sorting.
Results
2
The proportion of plasma cells in bone marrow samples was (14.5±13.8)% and (51.5±30.3)%
respectively
in the direct detection group and CD45 sorting group
with that of the latter group 7.69 times higher than that of the former group (
P
＜0.001). Direct FISH indicated 17 cases of cytogenetic abnormality
while FISH after CD45 sorting found 39 patients having cytogenetic abnormalities (
P
＜0.001). The positive rates of IgH rearrangement
13q14 deletion
1q21 amplification and 17p deletion were all increased significantly after sorting. According to mSMRAT3.0 standard
onl
y 28.3% of the patients were identified at high risk before sorting
but the number was increased significantly after sorting (accounting for 53.3%
P
=0.002).
Conclusion
2
The new CD45 negative cell sorting technology is simple and easy to operate
and can improve the detection rate of cytogenetic abnormalities and accurately carry out risk stratification for guiding treatment in MM patients. Thus
this technology is worthy of promotion in primary hospitals.
关键词
Keywords
references
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